Comparison of the action of transforming growth factor-β1 and interleukin-1β on matrix metabolism in the culture of porcine endothelial cells

Skop, B; Sobczak, Andrzej; Drózdz, M; Kotrys-Puchalska, E

doi:10.1016/0300-9084(96)82640-x

Cited by 4 publications

(3 citation statements)

References 15 publications

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“…Our results showed down-regulation of a number of sulfotransferases and other enzymes involved in proteoglycan synthesis. This is consistent with reduced proteoglycan synthesis in porcine endothelial cells following IL1β treatment as shown by Skop et al (1996). Genes related to chromosome organization are also overrepresented among our list of genes differentially expressed following IL1β treatment.…”

Section: Discussionsupporting

confidence: 83%

Gene Expression of Endothelial Cells due to Interleukin-1 Beta Stimulation and Neutrophil Transmigration

Williams¹,

Kataoka²,

Sakurai³

et al. 2008

Endothelium

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During the inflammatory response, endothelial cell (EC) functions and mechanics change dramatically. To understand these responses, the authors analyzed changes in EC gene expression in an in vitro model of inflammation using cDNA microarrays. After interleukin-1 beta (IL1beta) stimulation, over 2500 genes were differentially expressed, of which approximately 2000 had not been previously identified by microarray studies of IL1beta stimulation in human umbilical vein endothelial cells (HUVECs). Functional grouping of these genes according to gene ontologies revealed genes associated with apoptosis, cell cycle, nuclear factor (NF)-kappa B cascade, chemotaxis, and immune response. Interestingly, claudin-1, known to exist in endothelial cell-cell junctions was up-regulated, but claudin-5 and occludin, which also exist in EC junctions, were down-regulated. Pre-b-cell colony enhancing factor (PBEF), a cytokine which may play a role in regulating endothelial permeability, was also up-regulated following IL1beta stimulation. Neutrophil transmigration across IL1beta-stimulated ECs did not induce changes in EC gene expression as strongly as IL1beta stimulation alone. Nineteen genes after 1 h and 22 genes after 3 h of neutrophil application were differentially expressed. These results indicate that, in terms of transcriptional effects on ECs, neutrophil transmigration is a relatively small perturbation in comparison to the background of large scale changes induced in ECs by cytokine stimulation. Supplementary materials are available for this article. Go to the publisher's online edition of Endothelium for the following free supplementary resources: supplementary figures and tables.

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Section: Discussionsupporting

confidence: 83%

Gene Expression of Endothelial Cells due to Interleukin-1 Beta Stimulation and Neutrophil Transmigration

Williams¹,

Kataoka²,

Sakurai³

et al. 2008

Endothelium

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“…In addition to upregulating inflammatory mediators, we found that IL-1␤ also induced an increased synthesis of sulfated GAGs by the GECs, an effect that can be a part of the cell defense to maintain the permselective properties of the glomerular barrier (20). There may, however, be differences between micro-and macrovascular endothelium, because the synthesis of GAGs was downregulated when porcine aortic endothelial cells were stimulated with IL-1␤ (36).…”

Section: Discussionmentioning

confidence: 99%

Synthesis of sulfated proteoglycans by bovine glomerular endothelial cells in culture

Sörensson

Björnson

Ohlson

et al. 2003

American Journal of Physiology-Renal Physiology

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It has been suggested that proteinuria is caused by alterations of the charge selectivity of the basement membrane and/or the epithelial cell layer (podocytes). However, recent findings suggest that the endothelial luminal surface coat, consisting of proteoglycans with their connected glycosaminoglycan (GAG) branches and glycoproteins, may contribute to the permselectivity. Therefore, we wanted to investigate the effects on endothelial GAG synthesis during normal and pathological conditions. We treated glomerular endothelial cell cultures with puromycin aminonucleoside (PAN, a nephrosis-inducing agent) or interleukin-1beta (IL-1beta) for a total of 72 h and compared the metabolic turnover and incorporation of [(35)S]sulfate during the last 2 days. In control cultures, the GAG content in the media supernatants increased 66 +/- 6% (mean +/- SE) between 12 and 42 h of incubation with radioactivity (P < 0.01, n = 8). The content of (35)S-labeled GAGs in the media was reduced by 31 +/- 1% by PAN (P < 0.001, n = 8) and increased by 141 +/- 15% by 10 U/ml IL-1beta (P < 0.01, n = 8). Treatment with enzymes revealed a dominance of heparan, chondroitin, and dermatan sulfate GAGs. Thus the glomerular endothelial cell production of GAGs was increased by IL-1beta and reduced by PAN. Therefore, it is conceivable that certain nephrotic conditions may be due to endothelial dysfunction, rather than other renal causes.

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“…In fact, we have shown that Ca-SP inhibits the proliferation of vascular endothelial cells by inducing a lower ability to respond to stimulation by endogenous basic fibroblast growth factor. 15) Although it has been reported that endothelial proteoglycan synthesis is modulated by several factors including transforming growth factor-β 1 , 10) interleukin-1β 22) and tumor necrosis factor-α, 23) little is known about the regulation of the secretion. It was suggested that activation of protein kinase C stimulates the release of either HSPGs and CS/DS PGs from cultured vascular endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of the Association of Proteoglycans with Cultured Vascular Endothelial Cell Layers by Calcium and Sodium Spirulan.

Kaji

Shimada

Yamamoto

et al. 2002

JOURNAL OF HEALTH SCIENCE

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Comparison of the action of transforming growth factor-β1 and interleukin-1β on matrix metabolism in the culture of porcine endothelial cells

Cited by 4 publications

References 15 publications

Gene Expression of Endothelial Cells due to Interleukin-1 Beta Stimulation and Neutrophil Transmigration

Gene Expression of Endothelial Cells due to Interleukin-1 Beta Stimulation and Neutrophil Transmigration

Synthesis of sulfated proteoglycans by bovine glomerular endothelial cells in culture

Inhibition of the Association of Proteoglycans with Cultured Vascular Endothelial Cell Layers by Calcium and Sodium Spirulan.

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