Comparison of test performance of biochemical parameters in semiautomatic method and fully automatic analyzer method

Kumari, Suchitra; Bahinipati, Jyotirmayee; Pradhan, Tapaswini

doi:10.4103/jfmpc.jfmpc_94_20

Cited by 5 publications

(5 citation statements)

References 17 publications

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“…All patients were assessed for their demographic profile and screened for their fasting blood glucose (FBG), gamma-glutamyl transferase (GGT), albumin (ALB), blood urea nitrogen (BUN), bicarbonate (HCO 3 ), uric acid, and bilirubin using a semi-automated clinical chemistry analyzer as described before ( Kumari et al, 2020 ).…”

Section: Methodsmentioning

confidence: 99%

Role of Oxidative Stress and the Identification of Biomarkers Associated With Thyroid Dysfunction in Schizophrenics

et al. 2021

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Background: Schizophrenia is associated with a deficiency of dietary antioxidants like vitamin B6, B9, and B12 resulting in defective methylation leading to hyperhomocysteinemia. Hyperhomocysteinemia causes mitochondrial DNA damage, oxidative stress, vascular damage, and lipid peroxidation. Oxidative stress and increase in reactive oxygen species result in 8-oxodG production which induces apoptosis of both astrocytes and thyrocytes thus predisposing them to thyroid dysfunction and neurodegeneration. Furthermore, the presence of excessive free radicals increases thyroid thermogenesis causing hyperthyroidism or its excess may cause hypothyroidism by inhibiting iodide uptake. In the present study, we evaluated the various biomarkers associated with thyroid dysfunction in schizophrenics.Materials and Methods: 288 patients suffering from schizophrenia and 100 control subjects were screened for liver function tests (LFTs) such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), and total bilirubin (TB). Also, the stress markers, namely malondialdehyde (MDA), homocysteine, cysteine, methionine, the thyroid profile including triiodothyronine (T3), thyroxine (T4), thyroid-stimulating hormone (TSH), thyroxine peroxide antibody (TPO-Ab); TSH receptor-Ab (TSHr-Ab), dietary antioxidants, lipids, cytokines, aminoacids and hormones, vitamins and trace elements, and other biochemical parameters.Results: The LFTs showed elevated levels of ALT (45.57 ± 4.87 Vs. 26.41 ± 3.76 U/L), AST (40.55 ± 1.34 Vs. 21.92 ± 3.65 U/L), ALP (121.54 ± 4.87 Vs. 83.76 ± 5.87 U/L), and total bilirubin (2.63 ± 0.987 Vs. 1.10 ± 0.056 mg/dl), in schizophrenics than controls. Increased levels of MDA (3.71 ± 0.967 Vs. 1.68 ± 0.099) and homocysteine (17.56 ± 2.612 Vs. 6.96 ± 1.987 μmol/L were observed in schizophrenics compared to the controls, indicating increased stress. Levels of cysteine and methionine were decreased in schizophrenics than the controls (1.08 ± 0.089 Vs. 4.87 ± .924 μmol/L and 17.87 ± 1.23 Vs. 99.20 ± 5.36 μmol/L). The levels of TPO-Ab (IU/ml), Tg-Ab (pmol/L), and TSHr-Ab (IU/L) were observed to be higher in the patients’ group as compared to control subjects (9.84 ± 2.56 Vs. 5.81 ± 1.98, 55.50 ± 2.98 Vs. 32.95 ± 2.87 and 2.95 ± 0.0045 Vs. 1.44 ± 0.0023 respectively). Levels of Vitamin B6, B9, and B12 were also significantly decreased in the patients compared to the healthy controls.Conclusion: The schizophrenics, demonstrated altered liver function, increased stress markers, and decreased dietary antioxidants. Reduced primary and secondary antioxidant levels, may result in hyperhomocysteinemia and cause further DNA and mitochondrial damage. Therefore, homocysteine and/or prolactin levels may serve as candidate prognostic markers for schizophrenia. Also, both neurological symptoms and the susceptibility to thyroid disorders may be prevented in the initial stages of this debilitating disorder by appropriate dietary supplementation of antioxidants which can rectify a reduction in primary and secondary antioxidants, and disturbed prolactin-serotonin-dopamine interactions in schizophrenics.

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Section: Methodsmentioning

confidence: 99%

Role of Oxidative Stress and the Identification of Biomarkers Associated With Thyroid Dysfunction in Schizophrenics

et al. 2021

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“…The technique known as “spitting” was used to collect the saliva that had not been stimulated. 18 The collection of saliva samples took place in the morning, specifically between the hours of 9 and 11 a.m. During this time samples were collected, the patient was instructed to remain in the dental chair with her head inclined forward and not to speak, swallow. The patients was instructed to spit their saliva into a clean graduated container once every minute for approximately 10 mins.…”

Section: Methodsmentioning

confidence: 99%

“…15 , 17 The endpoint method was used to estimate total protein using pyrogallol red dye. 18 , 19 The values were given in milligrams per deciliter.…”

Section: Methodsmentioning

confidence: 99%

Efficacy of nonsurgical periodontal therapy affecting salivary biomarkers in non-diabetic and type 2 diabetic periodontitis patients. An observational study

Hungund

Desai

Shah³

et al. 2023

Journal of Oral Biology and Craniofacial Research

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“…Mean Cell Hemoglobin (MCH): calculated using a formula, MCH = (Hb×10) ÷ RBCs (pg). Mean Cell Hemoglobin Concentration (MCHC): calculated by the formula, MCHC = (Hb×100) ÷ PCV (g/dL) [12][13][14][15] .…”

Section: Evaluation Of Hematological Parametersmentioning

confidence: 99%

“…A blood sample was centrifuged at 1000 rpm for 15 min, and the serum was collected to measure SGPT and SGOT. SGPT and SGOT were estimated using a previously published Prietest Touch Plus Biochemistry Analyzer method 15 .…”

Section: Evaluation Of Hematological Parametersmentioning

confidence: 99%

Evaluating the Effect of <i>Moringa concanensis</i> on Aluminium Chloride-Induced Anemia in Wistar Rats

Kumar,

Hariprasad,

Redhwan

et al. 2024

JNR

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Anemia, a widespread global health challenge, can be induced through exposure to deleterious substances such as aluminum chloride. The present investigation explores the potential ameliorative effects of Moringa concanensis - a plant acknowledged for its myriad medicinal virtues-against anemia induced by aluminium chloride. The study objective was to scrutinize the impact of Moringa concanensis on aluminium chloride-induced anemia in Wistar rats. In the experimental design, thirty Wistar rats were randomly distributed into five distinct groups: a normal control group (untreated), a diseased control group (administered with aluminium chloride at a dose of 0.5 mg/kg body weight), a standard group (treated with Ferrous ascorbate at 30 mg/kg body weight), and two groups receiving low and high doses of Moringa concanensis (200 mg/kg and 400 mg/kg body weight, respectively). All groups, with the exception of the normal control, were exposed to aluminium chloride at a dosage of 0.5 mg/kg body weight over a span of 14 days. Hematological indicators were evaluated following standard methodologies, serum ferritin levels were assessed through Electrochemiluminescence immunoassay (ECLIA), and vitamin B12 concentration was quantified using atomic absorption spectroscopy. Furthermore, histopathological alterations were identified through Hematoxylin and Eosin staining procedures. Statistical data were interpreted through one-way ANOVA, succeeded by Tukey’s post hoc analysis, considering a p-value below 0.05 as statistically significant. Upon 21 days of continuous treatment with Moringa concanensis, both low and high-dose groups exhibited elevation in hematological parameters, serum ferritin, total iron-binding capacity, and vitamin B12 in comparison to the diseased control group. Noteworthy findings were observed in the high-dose group (400 mg/kg body weight), displaying significant improvement compared to the diseased control group (P<0.001). Remarkably, the high-dose regimen restored hematological parameters to baseline levels and mirrored the efficacy observed with the standard drug (Ferrous ascorbate). These empirical findings underscore the potential of Moringa concanensis as a promising therapeutic candidate for the alleviation of aluminium chloride-induced anemia. These results pave the way for future research endeavors to unravel the precise mechanisms driving these protective effects.

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Comparison of test performance of biochemical parameters in semiautomatic method and fully automatic analyzer method

Cited by 5 publications

References 17 publications

Role of Oxidative Stress and the Identification of Biomarkers Associated With Thyroid Dysfunction in Schizophrenics

Role of Oxidative Stress and the Identification of Biomarkers Associated With Thyroid Dysfunction in Schizophrenics

Efficacy of nonsurgical periodontal therapy affecting salivary biomarkers in non-diabetic and type 2 diabetic periodontitis patients. An observational study

Evaluating the Effect of <i>Moringa concanensis</i> on Aluminium Chloride-Induced Anemia in Wistar Rats

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