Comparison of term and preterm labor procalcitonin and leukocyte cell volume, conductivity and light scatter (VCS) parameters in order to demonstrate the impact of inflammation on the triggering mechanisms of preterm uterin contractions

Ünal, Canan; Karataş, Esra; Fadıloğlu, Erdem; Portakal, Oytun; Beksaç, Mehmet Sinan

doi:10.1111/jog.14216

Cited by 5 publications

(3 citation statements)

References 42 publications

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“…The production of inflammatory cytokines caused by inflammatory activation is closely related to the maintenance of uterine quiescence and the induction of uterine activation during pregnancy (6,7). The increased production of proinflammatory cytokines is related to uterine activation and the occurrence of PTB, whereas anti-inflammatory cytokines play an essential role in uterine quiescence during gestation (8,9).…”

Section: Introductionmentioning

confidence: 99%

miR‑199a‑3p suppresses cervical epithelial cell inflammation by inhibiting the HMGB1/TLR4/NF‑κB pathway in preterm birth

et al. 2020

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Preterm birth (PTB) is the primary cause of neonatal mortality worldwide. Infection and inflammation are considered to be the primary causes of PTB. cervical remodeling is an important step in the process of preterm delivery, and the destruction of the cervical epithelial barrier and inflammation are important triggers of cervical remodeling. The aim of the present study was to determine the effect and underlying mechanism of microrna (mir)-199a-3p/high-mobility group box 1 protein (HMGB1) signaling in cervical epithelial inflammation in PTB. The results of this study revealed that miR-199a-3p was significantly decreased in cervical epithelial tissue samples from patients in both the preterm labor and preterm premature rupture of membrane groups. This decrease was also observed in tissue samples from a lipopolysaccharide (lPS)-induced PTB mouse model and in lPS-induced ectocervical and endocervical cells. Whereas, the expression of HMGB1 and toll-like receptor 4 (TLR4) was significantly increased, which was associated with the upregulation of interleukin (il)-1β and tumor necrosis factor (TnF)-α expression. Furthermore, overexpression of mir-199a-3p significantly suppressed the expression and activation of HMGB1 and Tlr4/nF-κB signaling, and decreased the levels of il-1β and TnF-α in vitro and in vivo. additionally, overexpression of HMGB1 and/or Tlr4 reversed the anti-inflammatory effects of mir-199a-3p mimics in vitro and in vivo. These results indicate that miR-199a-3p acts as a negative inflammatory regulator in PTB by targeting HMGB1 to regulate the Tlr4/nF-κB pathway.

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Section: Introductionmentioning

confidence: 99%

miR‑199a‑3p suppresses cervical epithelial cell inflammation by inhibiting the HMGB1/TLR4/NF‑κB pathway in preterm birth

et al. 2020

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“…Preterm birth is one of the major factors leading to high rates of neonatal complications 15,16 . In routine practice, most of the preterm newborns are admitted to neonatal intensive care units in order to take necessary precautions and/or to prevent unwanted complications 17,18 . A wide spectrum of neonatal problems due to variations in the gw age at birth and etiological factors behind preterm birth necessitate patient‐specific intensive care protocols.…”

Section: Discussionmentioning

confidence: 99%

Impact of preterm birth on the cellular characteristics of neonatal buccal cells

et al. 2021

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Objective To demonstrate the impact of preterm birth on the cytological, cytomorphometrical, and nuclear parameters of neonatal buccal smears. Methods This study consisted of Early Preterm Neonates (EPN; ≤34th gestational week [gw]; n = 36), Late Preterm Neonates (LPN; 34th to <37th gw; n = 46), and Term Neonates (control; ≥37th gw; n = 56). Cytological evaluation and buccal cytome assay were performed using Papanicolaou and Feulgen methods, respectively. Results Cytological evaluation demonstrated that smear background was cleaner (P < .05) and there were less macrophages in the control group (P < .001). Cyto‐morphometric analysis showed that the measurements of nuclear diameter, nuclear area, and nucleus‐to‐cytoplasm ratio were higher in the preterm (EPN and LPN) versus the control groups (P = .016, P < .001, and P < .001, respectively). We also demonstrated that staining intensity of the nucleus and cytoplasm were less intense in the EPN and LPN groups (P < .001). There was no statistically significant difference between the EPN and LPN groups for any parameters (P > .05). Buccal cytome assay showed that nuclear buds were more prevalent in term newborns compared to preterm neonates (P < .001). Conclusions Morphological and cytological properties of neonatal buccal cells are influenced by preterm birth status, and buccal smears may be used as a tool to detect biological markers of neonatal health problems.

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“…Various maternal risk factors (immunological, metabolic, inflammatory, toxic) may cause placental inflammation-related obstetric complications such as preterm birth. 1 2 15 27 Further studies are necessary to understand the relationship between inflammatory processes and Wnt/β-catenin signaling pathway in preterm neonates.…”

Section: Discussionmentioning

confidence: 99%

Impact of Prematurity on the Buccal Epithelial Cells of the Neonates via Wnt/Beta-Catenin Signaling Pathway and Apoptosis

et al. 2021

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Objective Understanding the reflections of prematurity is necessary for the management of neonatal complications. We focused on the impact of prematurity and related “maternal risk factors/obstetric complications” on buccal cells of the neonates via evaluation of the Wnt/β-catenin signaling pathway and apoptosis. Study Design This study consisted of “early preterm neonates (EPN) (≤34th gestational week [gw]) (n = 36),” “late preterm neonates (LPN) (34th– < 37th gw) (n = 46),” and “term neonates (control) (≥37th gw) (n = 56).” Cohort was also subclassified according to the presence of maternal risk factors, obstetric complications, and neonatal complications. Wnt/β-catenin signaling and caspase-3 activation pathways were studied immunocytochemically. Results Wnt/β-catenin signaling positivity was statistically more frequent at buccal smears of the EPN and LPN groups compared with controls (p < 0.001). The cutoff for gestational age at delivery in receiver operating characteristic curve with the best balance of sensitivity (67.4%) and specificity (67.3%) was 35.8th gw for determining the reduction of Wnt/β-catenin signaling positivity (p < 0.001). The study demonstrated that obstetric complications significantly affected the activity of signaling, while maternal risk factors do not have any effect on Wnt/β-catenin signaling pathway (p = 0.003 and p = 0.828, respectively). This study also demonstrated a significant relationship between Wnt/β-catenin signaling pathway and the presence of neonatal complications (p = 0.015). Conclusion Dynamic characteristics of buccal cells are influenced by prematurity and related obstetric and neonatal problems. Buccal smear is a good tool to investigate the impact of prematurity and obstetric problems on perinatal outcome. Key Points

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Comparison of term and preterm labor procalcitonin and leukocyte cell volume, conductivity and light scatter (VCS) parameters in order to demonstrate the impact of inflammation on the triggering mechanisms of preterm uterin contractions

Cited by 5 publications

References 42 publications

miR‑199a‑3p suppresses cervical epithelial cell inflammation by inhibiting the HMGB1/TLR4/NF‑κB pathway in preterm birth

miR‑199a‑3p suppresses cervical epithelial cell inflammation by inhibiting the HMGB1/TLR4/NF‑κB pathway in preterm birth

Impact of preterm birth on the cellular characteristics of neonatal buccal cells

Impact of Prematurity on the Buccal Epithelial Cells of the Neonates via Wnt/Beta-Catenin Signaling Pathway and Apoptosis

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