Comparison of ten media for the enumeration of yeasts in dairy products

Welthagen, J.J.; Viljoen, Bennie C.

doi:10.1016/s0963-9969(97)00044-6

Cited by 16 publications

(2 citation statements)

References 28 publications

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“…For Streptococcus thermophilus count, M17 agar (Merck, Darmstadt, Germany) medium was used, and incubated for 48 h anaerobically at 37°C (Tharmaraj and Shah, 2003). Yeast Glucose Chloramphenicol Agar was used to determine the yeast and mould count, and the cultured Petri dishes were incubated at 25°C for 3 -5 d. The yeast and mould colonies that developed on the medium were counted separately and presented as total yeast and mould count (Welthagen and Viljoen, 1997).…”

Section: Microbiological Countsmentioning

confidence: 99%

The impact of medicinal and aromatic plant addition on antioxidant, total phenolic, antimicrobial activities, and microbiological quality of Mozzarella cheese

Yerlıkaya¹,

Akan²,

Bayram³

et al. 2021

IFRJ

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In the present work, the antioxidant, antimicrobial activities, total phenolic content, and microbiological quality of Mozzarella cheeses added with medicinal and aromatic plants namely rosemary (Rosmarinus officinalis L.), basil (Ocimum basilicum L.), peppermint (Mentha piperita L.), and Turkish oregano (Origanum onites L.) were investigated. Results demonstrated that peppermint and Turkish oregano increased antioxidant and antimicrobial activities, while Turkish oregano and basil increased the total phenolic content of the Mozzarella cheese samples. Mozzarella cheese samples added with Turkish oregano exhibited antimicrobial activity against Escherichia coli, Listeria monocytogenes, and Enterococcus faecalis during 30 days of storage. Mozzarella cheese sample added with rosemary and peppermint exhibited antimicrobial activity against Staphylococcus aureus in early storage, and Mozzarella cheese sample added with rosemary and Turkish oregano exhibited antimicrobial activity against Bacillus cereus on the 10th day of storage. It was determined that the highest activity against the tested microorganisms was observed in Mozzarella cheese sample added with Turkish oregano and peppermint. It was also determined that Lactobacillus spp. and Streptococcus thermophilus counts were above log 4 CFU/g for 30 days. The addition of medicinal and aromatic plants to the Mozzarella cheese samples did not affect yeast and mould development, and the yeast and mould counts increased during the storage period.

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Section: Microbiological Countsmentioning

confidence: 99%

The impact of medicinal and aromatic plant addition on antioxidant, total phenolic, antimicrobial activities, and microbiological quality of Mozzarella cheese

Yerlıkaya¹,

Akan²,

Bayram³

et al. 2021

IFRJ

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“…The basic principle behind the HPC method is that living fungi can grow on the culture medium, and the cultivability represents their viability . Different culture media have been selected for the fungal isolation, purification, and enumeration of fungal colonies in water. , As reported, dichloran rose bengal chloramphenicol (DRBC) agar, potato dextrose agar (PDA), dichloran 18% glycerol (DG-18) agar, rose bengal (RB) agar, and yeast extract dextrose chloramphenicol (YDC) agar are widely utilized for the enumeration of fungi. − …”

Section: Introductionmentioning

confidence: 99%

Comprehensive Comparisons of Five Kinds of Culture Media for Cultivating Fungal Spores at Different Viable States in Water

Chang,

Xu,

et al. 2023

ACS EST Water

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At present, there is no unified culture medium to quantify the cultivability of fungal spores in water, making comparisons between studies difficult. This study intended to select an appropriate medium to obtain a lower percentage of viable but nonculturable Aspergillus spores at different viable states (freshly prepared, impaired-treated) and fungi in actual water. Five kinds of media ((dichloran rose bengal chloramphenicol (DRBC) agar, potato dextrose agar (PDA), dichloran 18% glycerol (DG-18) agar, rose bengal (RB) agar, and yeast extract dextrose chloramphenicol (YDC) agar) were selected. To obtain the result quickly and colony counting clearly for freshly prepared Aspergillus spores, the optimal incubation time was determined, and the cultivating effectiveness of media was: DRBC > RB > YDC > DG-18 > PDA. The DRBC medium exhibited better performance in quantifying impaired spores treated by ultraviolet or chlorine. Besides, RB medium was optimal for fungal enumeration in actual water. DRBC and RB media exhibited higher potential capacity in cultivating Aspergillus spores at different viable states because the existence of rose bengal restricted the size of the colony and the lower content of glucose limited the rapid growth of colonies. This study provides meaningful information for selecting a suitable medium to quantify fungal spores at different viable states.

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