Comparison of telomerase activity and GSTP1 promoter methylation in ejaculate as potential screening tests for prostate cancer

Suh, ChongHyun; Shanafelt, Tait D.; May, Donald J.; Shroyer, Kenneth R.; Bobak, Joanna B.; Crawford, E. David; Miller, Gary J.; Markham, Neil E.; Glodé, L. Michael

doi:10.1006/mcpr.2000.0307

Cited by 60 publications

(22 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[7][8][9][10][11][12] Furthermore, loss of GSTP1 function seems to occur very early in prostatic carcinogenesis, as loss of GSTP1 expression and GSTP1 CpG island DNA hypermethylation have been detected in the majority of prostatic intraepithelial neoplasia lesions. 13 The data presented in this study, which focused on localized PCA removed at prostatectomy, revealed that somatic GSTP1 defects, whether CpG island hypermethylation or gene deletions, were present in all of the PCA cases studied.…”

Section: Discussionmentioning

confidence: 99%

GSTP1 CpG Island Hypermethylation Is Responsible for the Absence of GSTP1 Expression in Human Prostate Cancer Cells

Lin

Tascilar

Lee

et al. 2001

The American Journal of Pathology

215

134

View full text Add to dashboard Cite

GSTP1CpG island hypermethylation is the most common somatic genome alteration described for human prostate cancer (PCA); lack of GSTP1 expression is characteristic of human PCA cells in vivo. We report here that loss of GSTP1 function may have been selected during the pathogenesis of human PCA. Using a variety of techniques to detect GSTP1 CpG island DNA hypermethylation in PCA DNA, we found only hypermethylated GSTP1 alleles in each PCA cell in all but two PCA cases studied. In these two cases, CpG island hypermethylation was present at only one of two GSTP1 alleles in PCA DNA. In one of the cases, DNA hypermethylation at one GSTP1 allele and deletion of the other GSTP1 allele were evident. In the other case, an unmethylated GSTP1 allele was detected, accompanied by abundant GSTP1 expression. GSTP1 CpG island DNA hypermethylation was responsible for lack of GSTP1 expression by LNCaP PCA cells: treatment of the cells with 5-azacytidine (5-aza-C) , an inhibitor of DNA methyltransferases, reversed the GSTP1 promoter DNA hypermethylation, activated GSTP1 transcription, and restored GSTP1 expression. GSTP1 promoter activity, assessed via transfection of GSTP1 promoter-CAT reporter constructs in LNCaP cells, was inhibited by SssI-catalyzed CpG dinucleotide methylation. Remarkably, although selection for loss of GSTP1 function may be inferred for human PCA,

show abstract

Section: Discussionmentioning

confidence: 99%

GSTP1 CpG Island Hypermethylation Is Responsible for the Absence of GSTP1 Expression in Human Prostate Cancer Cells

Lin

Tascilar

Lee

et al. 2001

The American Journal of Pathology

215

134

View full text Add to dashboard Cite

show abstract

“…Suh et al 45 were one of the first to show the potential of this methylated gene for the early detection of prostate cancer. Using ejaculates, these investigators found methylated GSTP1 in 4/9 patients with prostate cancer.…”

Section: Prostate Cancermentioning

confidence: 99%

“…27,[39][40][41][42][43][44][45][46][47][48][49][50][51][52][53][54][55][56] The measurement of the methylated genes in these fluids for the identification of subjects at high risk of developing cancer as well as aiding the detection of early malignancy is discussed below. …”

mentioning

confidence: 99%

Methylated genes as new cancer biomarkers

Duffy

Napieralski

Martens

et al. 2009

European Journal of Cancer

View full text Add to dashboard Cite

Methylated genes as new cancer biomarkersBrünner, Nils; Duffy, M.J; Napieralski, R.; Martens, J.W.M. ; Span, P.N. ; Spyratos, F.; Sweep, F.C.G.J.; Foekens, J.A. ; Schmitt, M. GSTP1 for aiding the early diagnosis of prostate cancer, methylated PITX2 for predicting outcome in lymph node-negative breast cancer patients and methylated MGMT in predicting benefit from alkylating agents in patients with glioblastomas. However, prior to clinical utilisation, these findings require validation in prospective clinical studies. Furthermore, assays for measuring gene methylation need to be standardised, simplified and evaluated in external quality assurance programmes. It is concluded that methylated genes have the potential to provide a new generation of cancer biomarkers.

show abstract

“…The majority of seminal spermatocytes were removed by density gradient centrifugation before the PEC-enriched fraction was stained for cell surface PSMA and intracellular PAP. PSMA þ /PAP þ PEC cells were sorted by FACS as previously described (26,27) and cell number and purity of each isolated PEC sample confirmed via cytospin analysis (listed in Table 1). Criteria of more than 90% purity and more than 10 3 cells (16) to which were adhered.…”

Section: Resultsmentioning

confidence: 99%

Regional Cell Proliferation in Microdissected Human Prostate Specimens after Heavy Water Labeling In Vivo: Correlation with Prostate Epithelial Cells Isolated from Seminal Fluid

Hayes

Simko

Holochwost

et al. 2012

Clinical Cancer Research

View full text Add to dashboard Cite

Purpose: Prostate cancer is detected with increasing frequency but has a highly variable natural history and prognosis and active surveillance of men with low-risk prostate cancer would benefit greatly from minimally invasive methods to identify progression. We describe here two novel in vivo metrics of cell proliferation in men with prostate neoplasia.Experimental Design: Three groups of men drank heavy water, a nonradioactive, stable isotopic tracer for 14 to 28 days: (i) healthy men, (ii) men scheduled for transrectal core needle biopsy, and (iii) men scheduled for radical prostatectomy. Prostate epithelial cells (PEC) were isolated from ejaculated seminal fluid in all subjects. Histologically graded lesions were microdissected from tissue slides obtained from subjects undergoing surgery and proliferation rates were measured from isolated cells via mass spectrometry.Results: Proliferation rates of seminal PEC in healthy men (0.10%-0.27%/d) were stable on repeat sampling. Rates above 0.34%/d were seen only in patients with cancer where rates increased progressively from normal tissue through benign prostate hyperplasia, prostate intraepithelial neoplasia, and tumor grades III and IV in all subjects. Seminal PEC kinetics correlated highly with the most proliferative microdissected region in each subject (r 2 ¼ 0.94).Conclusions: Prostate cell proliferation can be measured in vivo from microdissected histopathology sections or noninvasively from seminal fluid where the latter reflects the most proliferative region of the gland. This approach may allow monitoring of progression in men with low-risk prostate cancer.

show abstract

Comparison of telomerase activity and GSTP1 promoter methylation in ejaculate as potential screening tests for prostate cancer

Cited by 60 publications

References 16 publications

GSTP1 CpG Island Hypermethylation Is Responsible for the Absence of GSTP1 Expression in Human Prostate Cancer Cells

GSTP1 CpG Island Hypermethylation Is Responsible for the Absence of GSTP1 Expression in Human Prostate Cancer Cells

Methylated genes as new cancer biomarkers

Regional Cell Proliferation in Microdissected Human Prostate Specimens after Heavy Water Labeling In Vivo: Correlation with Prostate Epithelial Cells Isolated from Seminal Fluid

Contact Info

Product

Resources

About