Comparison of systemic inflammatory and hematology parameters in normal C57BI/6 and genetically diabetic db/db mice during local wound repair

Šitum, Kristina; Bokulić, Ana; Ivetić-Tkalčević, Vanesa; Parnham, Michael J.; Čužić, Snježana; Đurić, Koraljka; Glojnarić, Ines; Ševeljević-Jaran, Daša; Brajša, Karmen

doi:10.11613/bm.2007.009

Cited by 3 publications

(3 citation statements)

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“…Differential count of neutrophils showed significant higher levels in wounded mice compared to the normal group after 48 h of injury. These findings are comparable with [ 36 ] and [ 37 ] who showed that in full-thickness incision wounds in mice displayed infiltration of the tissue by neutrophils and macrophages for at least 13 days. Whereas application of MB-PEG or MB-PEG + laser along 21 days significantly induced a rise in the count of neutrophils compared to wounded and normal groups, these results are compatible with [ 10 ] who found that in MB mediated PDT; human neutrophils adhesion increases and does not modify myeloperoxidase release.…”

Section: Discussionsupporting

confidence: 91%

Photodynamic therapy mediated by methylene blue-loaded PEG accelerates skin mouse wound healing: an immune response

Hamed,

Al Balah,

Refaat

et al. 2024

Lasers Med Sci

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Purpose Conventional approaches for enhancing wound healing may not always yield satisfactory results. Instead, we test the effectiveness of a newly developed photodynamic therapy (PDT) that uses methylene blue (MB) loaded with polyethylene glycol (PEG) (MB-PEG) hydrogel to accelerate wound healing process in mice. Methods A dorsal skin incision with 6 mm punch which topically subjected to MB-PEG hydrogel and a low-level laser light of red light to assess the regeneration process of wounded skin. A total of 63 adult male CD1 mice divided into normal group (no treatment) and other wound groups received different treatments of laser (650 ± 5 nm and power intensity of 180 mW/cm2), MB-PEG, or PDT (MB-PEG followed by laser). The wound healing parameters were investigated by histological examination of the skin and measuring of proinflammatory cytokines at the early stage (48 h) and a late one on day 21. Results: at 48 h, the score of tissue granulation, inflammation, and angiogenesis process were markedly improved in wounded groups that received MB + PEG combined with laser compared to the group treated with laser alone. On day 21, a significant improvement of the inflammation was detected in the group treated with MB + PEG plus laser compared to the other groups. At 48 h, the upregulated serum levels of tumor necrosis factor (TNF)-α and interleukin (IL)-1β in the wound group were significantly (P < 0.001) reduced in the group treated with MB + PEG combined with laser. Conclusion MB-PEG based hydrogel improves and accelerates wound closure in the context of laser compared to either single treatment.

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Section: Discussionsupporting

confidence: 91%

Photodynamic therapy mediated by methylene blue-loaded PEG accelerates skin mouse wound healing: an immune response

Hamed,

Al Balah,

Refaat

et al. 2024

Lasers Med Sci

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“…The results of the hematological evaluation of the blood samples in table 1 showed significant changes in the following parameters; For example in the Treatment group II (MGR) increasing in the mean value of WBC these discoveries associate very well with a past report 31 , this was overwhelmingly accounted by an intensification in the circulating neutrophil infiltration 31 , perhaps as a result of recruitment or demarcation of neutrophils from bone marrow 32 . Also in the MGR treated group there was a significant increase in the mean value of blood platelets count, in agreements with former studies that found the increase in megakaryocytes may lead to increased blood platelets 33 , or platelet production can increase due to inflammatory disease 34 .…”

Section: Discussionmentioning

confidence: 62%

Assessment the effects of Mastic Gum Resin, Lawsonia Inermis and Quercus Brantii on Cutaneous Wound Healing in BALB/c Mice

Ali¹,

Marif²,

Raoof³

et al. 2021

IJFMT

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The request for more efficient and lower-cost therapeutic methods for wound healing remains a challenge for modern medicine. The goals of this investigation were to recognize and look at the impacts of Mastic Gum Resin, Lawsonia Inermis, and Quercus Brantii in wound healing by using histopathological study and blood parameters in BALB/c Mice. Mice were comprised into four groups: Control negative group (n=10), mice were not treated with plant suspension only applied with normal saline; Treatment group I, mice were applied by Mastic Gum Resin (MGR) (n=10), Treatment group II, mice were applied with the Quercus Brantii (n=10), and the last group, Treatment III which were applied with Lawsonia Inermis (LI) (n=10). One ml for each suspension of Mastic Gum Resin (MGR), Quercus Brantii (QB), and Lawsonia Inermis (LI) was applied to the wound directly without suturing for 4 days/week for about 3 weeks. Wound healing effects were evaluated by utilizing the hematological profile for each group with the histopathological study. The cutaneous wound in Mastic Gum Resin and Quercus Brantii treated groups were more effective in progressing wound healing than Lawsonia Inermis treated group regarding histological changes at day 8 and day 18, respectively and blood parameters at day 21.

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“…As well, the number of PLT and monocyte was higher on the 7th day after PRP and/or SCH treatment. The hematological changes could be attributed to inflammation as an initial response to injury (Situm et al 2007), which is localized to the site of injury and generalized as systemic physio-biochemical interchanges to prevent possible microbial infection of the wound and to stimulate cytokine release (Velnar et al 2009). Initiation of the inflammatory response arises principally via stimulated blood monocytes and tissue macrophages at the wound site and then induced inflammatory factors causing systemic changes (Mahre et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Platelet-rich plasma and/or sildenafil topical applications accelerate and better repair wound healing in rats through regulation of proinflammatory cytokines and collagen/TGF-β1 pathway

Gad

Hafez

El‐Sayed

2020

Environ Sci Pollut Res

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Platelet-rich plasma (PRP) composites of various cytokines and growth factors which have the potential to activate and speed the process of wound repair. Sildenafil also is a potent stimulator of angiogenesis which favors its potential effects on wound healing in several models. Existing work planned to examine the effectiveness of topical application of PRP and/or sildenafil citrate hydrogel (SCH) in a non-splinted excision skin wound model. Adult male rats were allocated into control, PRP, SCH, and PRP/ SCH groups. On the 7th and 14th days, blood and tissue samples were collected for hematobiochemical, histopathological, and immunohistochemistry analyses. PRP and/or SCH topical treatments caused an enhancement of wound healing parameters, including a rapid switch from inflammatory phase to connective tissue stage evident by less systemic hematological changes and decreased values of proinflammatory cytokines (IL-6, TNF-α, and IL-1β) and C-reactive protein (CRP) on the 7th or 14th days post-wounding. Also, tissue hydroxyproline, collagen, nitrite, and total protein contents were higher in therapeutically handled wounded rats. Histologically, PRP-and/or SCH-treated wounded rats exhibited less necrosis, inflammation, and fibrin with a higher level of granulation tissue formation on the 7th day post-wounding and abundant collagen remodeling, epithelization, and vascularization on the 14th day relative to control. Interestingly, combined PRP and SCH treatment was more efficient in wound healing scoring with less inflammation, more collagen remodeling, and more epithelization. Our findings confirm the effectiveness of PRP and/or SCH as a topical wound healing treatment, with better skin wound healing with their combination.

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Comparison of systemic inflammatory and hematology parameters in normal C57BI/6 and genetically diabetic db/db mice during local wound repair

Cited by 3 publications

References 0 publications

Photodynamic therapy mediated by methylene blue-loaded PEG accelerates skin mouse wound healing: an immune response

Photodynamic therapy mediated by methylene blue-loaded PEG accelerates skin mouse wound healing: an immune response

Assessment the effects of Mastic Gum Resin, Lawsonia Inermis and Quercus Brantii on Cutaneous Wound Healing in BALB/c Mice

Platelet-rich plasma and/or sildenafil topical applications accelerate and better repair wound healing in rats through regulation of proinflammatory cytokines and collagen/TGF-β1 pathway

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