Comparison of synchrotron radiation and conventional x-ray microcomputed tomography for assessing trabecular bone microarchitecture of human femoral heads

Chappard, Christine; Basillais, A.; Benhamou, Laurent; Bonassie, Alexandra; Brunet-Imbault, B.; Bonnet, Nicolas; Peyrin, Françoise

doi:10.1118/1.2256069

Cited by 68 publications

(42 citation statements)

References 43 publications

(62 reference statements)

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“…It is possible that the decrease in trabecular bone thickness would make the decreased voxel size have more influence on observed trabecular volume fraction. Previous examinations comparing synchrotron radiation and conventional microCT in animals have not presented quantitative results and thus do not allow for comparison (37).…”

Section: Discussionmentioning

confidence: 99%

Long-Term Dose Response of Trabecular Bone in Mice to Proton Radiation

et al. 2008

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Astronauts on exploratory missions will experience a complex environment, including microgravity and radiation. While the deleterious effects of unloading on bone are well established, fewer studies have focused on the effects of radiation. We previously demonstrated that 2 Gy of ionizing radiation has deleterious effects on trabecular bone in mice 4 months after exposure. The present study investigated the skeletal response after total doses of proton radiation that astronauts may be exposed to during a solar particle event. We exposed mice to 0.5, 1 or 2 Gy of whole-body proton radiation and killed them humanely 117 days later. Tibiae and femora were analyzed using microcomputed tomography, mechanical testing, mineral composition and quantitative histomorphometry. Relative to control mice, mice exposed to 2 Gy had significant differences in trabecular bone volume fraction (−20%), trabecular separation (+11%), and trabecular volumetric bone mineral density (−19%). Exposure to 1 Gy radiation induced a nonsignificant trend in trabecular bone volume fraction (−13%), while exposure to 0.5 Gy resulted in no differences. No response was detected in cortical bone. Further analysis of the 1-Gy mice using synchrotron microCT revealed a significantly lower trabecular bone volume fraction (−13%) than in control mice. Trabecular bone loss 4 months after exposure to 1 Gy highlights the importance of further examination of how space radiation affects bone.

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Section: Discussionmentioning

confidence: 99%

Long-Term Dose Response of Trabecular Bone in Mice to Proton Radiation

et al. 2008

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“…Micro-CT has proven to be of value for research involving bone [Wong et al, 1995;Davis and Wong, 1996;Nuzzo et al, 2002a, b;Postnov et al, 2003;Chappard et al, 2006] and dental hard tissues, enamel as well as dentine [Kinney et al, 1994;Davis and Wong, 1996;Hahn et al, 2004;Luedemann et al, 2005;Plotino et al, 2006;Huang et al, 2007;Willmott et al, 2007]. Micro-CT is potentially capable of providing quantitative data, but in order to achieve this a reliable means of calibration is required.…”

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confidence: 99%

A System of Calibrating Microtomography for Use in Caries Research

Schwass¹,

Swain²,

Purton³

et al. 2009

Caries Res

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Desktop microcomputed tomography (micro-CT) offers a non-invasive 3-dimensional analysis of structures and their physical properties. To date, the use of micro-CT has mostly involved qualitative observations, with the extent of quantitative analysis relying on automated internal calibration by the micro-CT control software. However, the value of such calibration is limited by machine drift. For an accurate quantitative use of micro-CT, it is recognized that external means of calibration are needed. A novel system of calibration standards, also known as ‘phantoms’, is presented. A range of low mineral concentration phantoms involving triethylene glycol dimethacrylate/glycerolate dimethacrylate resin mixed with commercial pure hydroxyapatite (HAP), from 0.07 to 1.05 g/cm³, was fabricated. Sintered HAP was impregnated with the same resin, producing phantoms with medium-level mineral concentrations up to 1.90 g/cm³. These phantoms were easy to create, proved accurate and stable with repeated use, and were found to mimic the composite nature of dental enamel and dentine structures under investigation.

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“…In fact, the study of bone architecture and density drove the early developments of micro-CT systems (Ruegsegger et al, 1996). When applied to in vitro studies, the spatial resolution of dedicated benchtop systems approaches that provided by synchrotron sources (Boyd et al, 2006;Chappard et al, 2006). For in vivo applications, the acquisition protocol is constrained by dose to the animal (Ford et al, 2003), leading to a reduction in spatial resolution.…”

Section: Bone Imagingmentioning

confidence: 99%