Comparison of Surface Plasmon Resonance, Resonant Waveguide Grating Biosensing and Enzyme Linked Immunosorbent Assay (ELISA) in the Evaluation of a Dengue Virus Immunoassay

Hu, Dongmei; Fry, Scott; Huang, Johnny X.; Ding, Xiaoyi; Qiu, Li-wen; Pan, Yuxian; Chen, Yue; Jin, Jing; McElnea, Catriona; Buechler, Joe; Che, Xin; Cooper, Matthew A.

doi:10.3390/bios3030297

Cited by 32 publications

(17 citation statements)

References 26 publications

(34 reference statements)

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“…Taking these parameters into consideration, both SPR and ELISA would be good methods. Because we want to use a system in the absence of flow [ 44 , 45 ], we investigated the use of ELISAs for the systematic study of protein-carbohydrate interactions. Nuances associated with SPR, such as rebinding and model fitting, may provide a complementary but different analysis than the ELISA’s reported here.…”

Section: Introductionmentioning

confidence: 99%

Glycodendrimers and Modified ELISAs: Tools to Elucidate Multivalent Interactions of Galectins 1 and 3

et al. 2015

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Multivalent protein-carbohydrate interactions that are mediated by sugar-binding proteins, i.e., lectins, have been implicated in a myriad of intercellular recognition processes associated with tumor progression such as galectin-mediated cancer cellular migration/metastatic processes. Here, using a modified ELISA, we show that glycodendrimers bearing mixtures of galactosides, lactosides, and N-acetylgalactosaminosides, galectin-3 ligands, multivalently affect galectin-3 functions. We further demonstrate that lactose functionalized glycodendrimers multivalently bind a different member of the galectin family, i.e., galectin-1. In a modified ELISA, galectin-3 recruitment by glycodendrimers was shown to directly depend on the ratio of low to high affinity ligands on the dendrimers, with lactose-functionalized dendrimers having the highest activity and also binding well to galectin-1. The results depicted here indicate that synthetic multivalent systems and upfront assay formats will improve the understanding of the multivalent function of galectins during multivalent protein carbohydrate recognition/interaction.

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Section: Introductionmentioning

confidence: 99%

Glycodendrimers and Modified ELISAs: Tools to Elucidate Multivalent Interactions of Galectins 1 and 3

et al. 2015

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“…In response to these challenges several groups have proposed biosensor technologies for NS1 quantification in formats compatible with decentralized diagnostics. Immunosensors based on immunospot assays using fluorescent nanoparticles 13 , surface plasmon resonance 14 , and electrochemical detection 15 16 have recently been presented. These technologies display a growing capacity to provide sensitive NS1 quantification.…”

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confidence: 99%

Quantification of NS1 dengue biomarker in serum via optomagnetic nanocluster detection

Antunes

Watterson

Parmvi

et al. 2015

Sci Rep

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Dengue is a tropical vector-borne disease without cure or vaccine that progressively spreads into regions with temperate climates. Diagnostic tools amenable to resource-limited settings would be highly valuable for epidemiologic control and containment during outbreaks. Here, we present a novel low-cost automated biosensing platform for detection of dengue fever biomarker NS1 and demonstrate it on NS1 spiked in human serum. Magnetic nanoparticles (MNPs) are coated with high-affinity monoclonal antibodies against NS1 via bio-orthogonal Cu-free ‘click’ chemistry on an anti-fouling surface molecular architecture. The presence of the target antigen NS1 triggers MNP agglutination and the formation of nanoclusters with rapid kinetics enhanced by external magnetic actuation. The amount and size of the nanoclusters correlate with the target concentration and can be quantified using an optomagnetic readout method. The resulting automated dengue fever assay takes just 8 minutes, requires 6 μL of serum sample and shows a limit of detection of 25 ng/mL with an upper detection range of 20000 ng/mL. The technology holds a great potential to be applied to NS1 detection in patient samples. As the assay is implemented on a low-cost microfluidic disc the platform is suited for further expansion to multiplexed detection of a wide panel of biomarkers.

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“…The additional push for one technology to monitor several biomarkers simultaneously for more effective and universal disease diagnosis is motivating recent advancements in multiplexed sensing capabilities. Many optical approaches have been utilized for POC applications including fluorescence [69][70][71][72], luminescence, absorbance, Forster energy transfer (FRET) [73], bioluminescence energy transfer (BRET) [74][75][76], surfaceplasmon resonance (SPR) [77,78], resonant Rayleigh scattering [79][80][81][82], geometric scattering [1], and Raman spectroscopic approaches [83][84][85][86][87][88][89]. Advancements in biochemical sensing methods, nanotechnology, and the miniaturization of optics have been key in improving spectroscopic platforms for biomarker detection.…”

Section: Optical Analyses For Facilitating Poc Technologiesmentioning

confidence: 99%

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

et al. 2017

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Point-of-care (POC) device development is a growing field that aims to develop low-cost, rapid, sensitive in-vitro diagnostic testing platforms that are portable, selfcontained, and can be used anywhere -from modern clinics to remote and low resource areas. In this review, surface enhanced Raman spectroscopy (SERS) is discussed as a solution to facilitating the translation of bioanalytical sensing to the POC. The potential for SERS to meet the widely accepted "ASSURED" (Affordable, Sensitive, Specific, Userfriendly, Rapid, Equipment-free, and Deliverable) criterion provided by the World Health Organization is discussed based on recent advances in SERS in vitro assay development. As SERS provides attractive characteristics for multiplexed sensing at low concentration limits with a high degree of specificity, it holds great promise for enhancing current efforts in rapid diagnostic testing. In outlining the progression of SERS techniques over the past years combined with recent developments in smart nanomaterials, high-throughput microfluidics, and low-cost paper diagnostics, an extensive number of new possibilities show potential for translating SERS biosensors to the POC.

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Comparison of Surface Plasmon Resonance, Resonant Waveguide Grating Biosensing and Enzyme Linked Immunosorbent Assay (ELISA) in the Evaluation of a Dengue Virus Immunoassay

Cited by 32 publications

References 26 publications

Glycodendrimers and Modified ELISAs: Tools to Elucidate Multivalent Interactions of Galectins 1 and 3

Glycodendrimers and Modified ELISAs: Tools to Elucidate Multivalent Interactions of Galectins 1 and 3

Quantification of NS1 dengue biomarker in serum via optomagnetic nanocluster detection

Surface enhanced Raman spectroscopy (SERS) for in vitro diagnostic testing at the point of care

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