Comparison of Surface Markers between Human and Rabbit Mesenchymal Stem Cells

Abstract

This study investigated whether there are marked differences in surface markers between rabbit and human mesenchymal stem cells (MSCs). Murine and rabbit MSCs have been reported to be CD90-negative. Rat MSCs have been reported to be CD71-negative. Our previous study also shows that rabbit MSCs are CD29-negative. However, human MSCs are generally considered to be CD29-, CD71-, and CD90-positive. Therefore, the surface markers of human MSCs might differ from those of other species. Rabbit bone marrow MSCs were obtained that had a multi-differentiation potential. The phenotype of these cells was studied using flow cytometry antibodies for 25 rabbit surface markers, namely, CD13, CD14, CD29, CD31, CD34, CD44, CD45, CD49d, CD49f, CD51, CD54, CD59, CD71, CD73, CD90, CD105, CD106, CD133, CD166, MHC I, MHC II, α-smooth muscle actin (α-SMA), cytokeratin, desmin, and vimentin. The phenotype of commercially available human MSCs was similarly studied using antibodies for human surface markers. CD14, CD31, CD34, CD45, CD49d, CD49f, CD51, CD54, CD71, CD106, CD133, MHC II, and cytokeratin were absent from both rabbit and human MSCs, while CD44, α-SMA, and vimentin were present on both cell lines. CD13, CD29, CD59, CD73, CD90, CD105, CD166, and MHC I were present on human MSCs, but not on rabbit MSCs. However, desmin was present on rabbit MSCs, but not on human MSCs. In total, the surface expression of nine markers differed between human and rabbit MSCs, whereas the surface expression of 16 markers was the same in the two cell lines.

Different RNA and protein expression of surface markers in rabbit amniotic fluid‐derived mesenchymal stem cells

“…For this reason, investigators tend to use a set of different markers typical of a particular MSC population in the identification of these. Therefore, in addition to the detection of cell surface markers, we chose to detect mesechymal stem cell specific intracellular markers previously detected in rabbit and human BM‐MSCs . The expression of vimentin, α‐SMA, and desmin we detected was very similar to that described in a study conducted on rabbit BM‐MSCs (97.42% ± 0.71%; 93.69% ± 3.23%; 59.65% ± 8.13% vs. 95.68% ± 3.58%; 95.10% ± 2.00%; 77.91% ± 1.78%).…”

“…An extensive study of human and rabbit MSC surface markers using RT‐PCR and flow cytometry should be conducted in the future . To the best of our knowledge, we are the first to report a discrepancy between mRNA levels and corresponding surface marker protein expression (as detected by species‐specific antibodies) in rAF‐MSCs.…”

Effect of prevascularization on in vivo vascularization of poly(propylene fumarate)/fibrin scaffolds

“…For the in vitro experiments, immunofluorescence staining was performed for CD31, an endothelial cell marker, and α-smooth muscle actin (αSMA), expressed by hMSCs [46]. Following fixation in 10% buffered formalin (Fisher Scientific, Pittsburgh, PA), samples were washed with PBS, permeabilized with 0.5% Triton-X in PBS (PBST), and blocked with 6% normal goat serum (MP Biomedicals, Solon, OH) for one hour.…”

Mesenchymal Stem Cells for Optimizing Bone Volume at the Dental Implant Recipient Site

“…Lee et al [27] have demonstrated that CD14, CD31, CD34, CD45, CD49d, CD49f, CD51, CD54, CD71, CD106, CD133, major histocompatibility complex (MHC II), cytokeratin, and desmin were absent from human MSCs, whereas CD13, CD29, CD44, CD59, CD73, CD90, CD105, CD166, MHC I,a-SMA, and vimentin were present on human MSCs. For human bone marrow stromal cells, common targets of negative antigene expression include CD2, CD3, CD11b/Integrin alpha M, CD14, CD15/Lewis X, CD16/F c gamma RIII, CD19, CD38, CD56/NCAM-1, CD66b/CEACAM-8, CD123/IL-3 R alpha, and CD235a/Glycophorin.…”