Comparison of Stratum Corneum and Hair Fibrous Proteins

Abstract

The Tris-urea-mercaptoethanol (pH 9) soluble fibrous proteins of hair and stratum corneum have been compared in a number of animals. The 2 tissues show different urea (pH 8.3) and SDS polyacrylamide gel electrophoretic patterns and vary in amino acid composition. Cyanogen bromide cleavage was done on human stratum corneum and hair fibrous proteins and found to give different fragments by electrophoresis. Antibodies to stratum corneum protein did not react with hair protein and vice versa. Although the 2 types of fibrous proteins have the same helical structure as shown by x-ray diffraction analysis, their differences in composition may be important for specific interactions with other macromolecules.

Hypotrichosis with Keratosis pilaris: Electrophoretical Study of Hair Fibrous Proteins from a Patient

“…but also to that reported for the nor mal human hair [5,19], This suggests that in the present study the hair SCM fibrous protein preparations analyzed were properly prepared. The amino acid composition of the HN-4' component from the patient was very similar to that of the HN-4 component from the normal individual (table 1).…”

“…Proteins were extracted from a hair specimen with 0.2 M Tris-HCl (pH 9.5) containing 8 M urea and 0.2 M 2-mercaptoethanol (5,15]. After centrifugation, the supernatant obtained was treated with iodoacetic acid to yield the S-carboxymcthylated (SCM) derivatives.…”

“…Biochemically considered, human hair as well as nails are members of the large family of homogeneous tissues, cmbryologically of epidermal origin, the mammalian hard cv-kcratins [4,5], Electron microscope and X-ray diffrac tion studies have shown that they are built in a common arrangement of filaments embedded in a nonfilamentous matrix. The filaments are composed of multiple subunit proteins of comparatively low cysteine content, which arc thus referred to as fibrous proteins or low-sulfur proteins [6][7][8].…”

Production and Proteomic Characterization of Pharmaceutical-Grade <i>Dermatophagoides pteronyssinus </i>and <i>Dermatophagoides farinae </i>Extracts for Allergy Vaccines

“…To define the optimal amino acid combination to use, we analyzed human skin scales by HPLC. The amino acid composition of human scales was found to compare with data available from the literature, as it relates to the human stratum corneum[35, 36] (data not shown). On the basis of this analysis, for the subsequent cultures we selected a mix of amino acid referred to as ‘synthetic aa mix’ (see ‘Materials and Methods’), commonly used in humans for parenteral nutrition.…”

Amounts of Fibrous Proteins and Matrix Substances in Hairs of Different Races

“…Exactly 100 mg of the washed specimen as weighed with an electric balance (model PC2000, Mettler Instrumente AG, Zurich) was suspended in 100 ml of a freshly prepared solution of 0.2M tris-HCI (pH 9.5), 8M urea, and 0.2M 2-mercaptoethanol (4,5). It was then stirred at 100rpm under nitrogen at 50°Cfor 1 hr in a water incubator (model M-I00 N , Taiyo Science Industrial Co., Tokyo).…”