Comparison of Str. griseus strains which produce streptomycin and those which do not

Szabó, Gábor; Barabás, Gy.; Valyi-Nagy, T

doi:10.1007/bf00540578

Cited by 29 publications

(7 citation statements)

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“…P"A. (8). When it was treated in the early stage of life cycle (24-h culture), the production of SM decreased (though in Fig.…”

Section: Resultsmentioning

confidence: 94%

“…52-1) strain was used. Its cultural and physiological characteristics, as well as the composition of the filtered soybean medium employed throughout the experiments, were previously described (8). Cultivation was carried out on a rotary shaker (250 rpm, 50-mm stroke distance) at 270C.…”

Section: Materlals and Methodsmentioning

confidence: 99%

“…We proved that streptidine (SD), a component of the SM molecule, is a constituent of the cell wall (2,10). This indicates that SM or its SD component plays a role in building up the cell wall network (1,(8)(9)(10). These findings prompted us to search for correlations between cell wall synthesis and SM production in S. griseus.…”

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confidence: 99%

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Effect of Penicillin on Streptomycin Production by Streptomyces griseus

Barabás

Szabó

1977

Antimicrob Agents Chemother

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The correlation between the biosynthesis of the cell wall and the formation of streptomycin (SM) in Streptomyces griseus, influenced by a specific inhibitor of cell wall synthesis, was investigated. Penicillin, in subinhibitory concentrations (1 to 5 ,ug/ml), was added to cultures of S. griseus in different stages of its life cycle. The inhibitor decreased SM production, when young cultures were treated; however, there was an increase in SM formation when penicillin was added to older cultures. The explanation for these findings is discussed in detail.The physiological role of streptomycin (SM) in the life of Streptomyces griseus has been investigated. We proved that streptidine (SD), a component of the SM molecule, is a constituent of the cell wall (2, 10). This indicates that SM or its SD component plays a role in building up the cell wall network (1,(8)(9)(10). These findings prompted us to search for correlations between cell wall synthesis and SM production in S. griseus. Use of specific inhibitors of cell wall synthesis in bacteria, such as penicillin, bacitracin, etc., seemed promising as an approach to the problem (Gy. Barabas, Folia Microbiol. 18:266, 1973). Since SD is a component of cell walls, one might expect that the inhibition of cell wall synthesis would change SM production.In this paper, we describe the effects on SM production by S. griseus cultures when penicillin is added at different stages in the S. griseus life cycle. MATERLALS AND METHODSStrains and cultivation. S. griseus LS-1 (code no. 52-1) strain was used. Its cultural and physiological characteristics, as well as the composition of the filtered soybean medium employed throughout the experiments, were previously described (8). Cultivation was carried out on a rotary shaker (250 rpm, 50-mm stroke distance) at 270C.Antibiotic assay. SM content of the fermentation liquid was determined by the agar-hole diffusion method against Bacillus subtilis ATCC 6633. The fermentation liquid was spun down, and the cellfree supernatants were diluted with 0.067 M phosphate buffer (pH 7.5) containing penicillinase (Neutrapen Riker Laboratories, Loughborough, England, in a final concentration of 100 ,ug/ml). The samples were incubated at 37°C for 1 h to inactivate penicillin. Then, four to seven replicates were pipetted in the holes of agar plates from every sample, and their mean was used for the calculation. The agar medium for antibiotic assay was prepared as follows: 5 g of meat extract, 1 g of Na2HPO4, 0.1 g of KH2PO4, and 0.5 g of peptone were dissolved in 100 ml of distilled water and boiled for 2 min. After filtration, 900 ml of distilled water was added, as well as 20 g of agar. The pH of the medium was adjusted to 7.0 after it had melted, and it was finally sterilized by autoclaving. The melted medium was inoculated with B. subtilis, poured into petri plates, and incubated at 37°C for 1 h. Samples were then pipetted into the holes. The inhibition zone of the antibiotic was measured after 20 h of incubation at 37°C. Control experiments showed ...

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“…P"A. (8). When it was treated in the early stage of life cycle (24-h culture), the production of SM decreased (though in Fig.…”

Section: Resultsmentioning

confidence: 94%

Section: Materlals and Methodsmentioning

confidence: 99%

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Effect of Penicillin on Streptomycin Production by Streptomyces griseus

Barabás

Szabó

1977

Antimicrob Agents Chemother

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“…S. griseus LS-1, an SM-producing strain (code number 52-1), and its SM-nonproducing mutant (code number 45) were used. The cultural and physiological characteristics of these strains, as well as the procedure for preparation of the SM-nonproducing mutant, have been published previously (8).…”

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confidence: 99%

“…Strains were cultivated in a soy bean liquid medium (8) in a rotatory shaker (250 rpm, 70-mm stroke distance) at 27 C.…”

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confidence: 99%

Transamidinase Activity of Hyphal Fractions of a Streptomycin-Producing Streptomyces griseus Strain and Enhancement of Enzyme Activity in a Nonproducing Mutant

Barabás

Szeszák

Szabó

1974

Antimicrob Agents Chemother

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The presence of transamidinase (TA-se) was demonstrated in Streptomyces griseus strain 45 which does not produce streptomycin (SM). This enzyme is involved in the biosynthesis of streptidine, a component of the SM molecule. TA-se is generally thought to be present only in SM-producing Streptomyces strains. The recent results are, however, in agreement with out previous data which showed the presence of streptidine in the isolated cell wall of SM-nonproducing strain 45. The TA-se activity of this strain is very low, but we were able to increase it by addition of arginine or by increasing the incubation with myo-inositol. The addition of these substances to the cultures of SM-nonproducing S. griseus strains, which hitherto were deficient in TA-se, may serve as a method for detecting the presence of this enzyme. In an incubation system in which the hyphae of an SM-producing strain of S. griseus had been blended and separated by density gradient centrifugation, certain hyphal fractions had been combined, and their SM production and TA-se activity had been measured, we were unable to find any close correlation between TA-se activity and SM-producing capacity. The hyphal fractions and their combination did not show strict parallelism between TA-se activity and the level of SM production.

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Protein patterns of Streptomyces griseus strains during development

Vitális

Békési

Valu

et al. 1984

J of Basic Microbiology

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(Bingegangen urn. 28. 7.1983) Protein patterns from mycelial extracts of Streptornyces griseus strains No. 45H and No. 52-1 were studied by one dimensional gradient PAGE with 20 cm run. The results are reproducible, the protein patterns are t,he same for the same developmental stage in a given strain. There are welldefined characteristic changes of the protein patterns during the life cycle. The proteins of spores show conspicuous differences compared to protein patterns of the old (72 h) mycelia in the same culture. There is no difference between protein patterns of spores from submerged and from solid media. The protein patterns of two closely related Streptomyces griseus strains significantly differ from each other. After pulse labelling with (I4C)-labelled protein hydrolyzate for 40 min, tho specific activities of the proteins show considerable differences. There are characteristic bands with low specific activity and others with high incorporation. The fluorograms of the 16, 40 and 64 h cultures show t h a t different proteins are being synthesized intensively at different ages of the culture.I n our earlier experiments studying the differentiation process of Streptomyces griseus, two definitely distinct phases of development were established (VITALIS et al. 1%3, 1981(VITALIS et al. 1%3, , B1a6 et al. 1979). The vegetative and reproductive phases are characterized by specific cytolriorphological and biochemical enzymatic markers. The switch to the reproductive phase is followed by the appearance of a series of features suggesting that the new proteins, which are necessary for the forniation of reproductive hyphae, may appear in groups, although both phases can be subdivided into stages which can he resolved by waves of enzyme activities and characteristic changes of the cytomorphology of the hyphae.On the basis of the above assumption we thought that the appearance of the coniplex markers of a given stage or even the two main phases would be accompanied by the production of a series of new proteins, and we expected to obtain patterns which might be characteristic of the coordinated expression of developmental programs or subprograms if they existed. Therefore, we chose to apply one dimensional SDS polyacrylarriide gel electrophoresis. With this method only about 150-200 diffcrent protein bands can he resolved, but as it is a relatively simple method, it makes possible to compare higher number of saiiiples from two different strains at different ages and of different subcellular fractions. Pulse labelling was applied to study the actually synthesized proteins by the mycelia at a given stage of the life cycle. Materials and nrethodsThe experiments presented here were curicd out with twoStreptarnyces yriveus strains. One of them is a well-differentiating, streptomycin-non-producing mutant: No. 45H, the other is a streptomycinproducing one: No. 52-1, which does not sporulate in submerged culture ( S Z A B~ et al. 1961et al. , VITALIS et ul. 1963. Strains were cultivated in liquid soy-bean medium, spores we...

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Comparison of Str. griseus strains which produce streptomycin and those which do not

Cited by 29 publications

References 4 publications

Effect of Penicillin on Streptomycin Production by Streptomyces griseus

Effect of Penicillin on Streptomycin Production by Streptomyces griseus

Transamidinase Activity of Hyphal Fractions of a Streptomycin-Producing Streptomyces griseus Strain and Enhancement of Enzyme Activity in a Nonproducing Mutant

Protein patterns of Streptomyces griseus strains during development

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