Comparison of steroidogenic gene expression in mummichog (Fundulus heteroclitus) testis tissue following exposure to aromatizable or non-aromatizable androgens

Rutherford, Robert; Lister, Andrea; MacLatchy, Deborah L.

doi:10.1016/j.cbpb.2018.09.001

Cited by 4 publications

(4 citation statements)

References 78 publications

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“…They were then exposed to different concentrations of 17MT (25, 50, and 100 ng/L, corresponding to groups MT25-M, MT50-M, and MT100-M, respectively; treatment groups) or to 0.0001% anhydrous ethanol (control group; Con-M) for 7 days. The concentration and duration of exposure were selected based on previous reports [ 4 , 5 , 6 ]. All experiments were performed in triplicate.…”

Section: Methodsmentioning

confidence: 99%

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Comparative Analysis of miRNA-mRNA Regulation in the Testes of Gobiocypris rarus following 17α-Methyltestosterone Exposure

Liu

Zhou

Yang

et al. 2023

IJMS

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17α-Methyltestosterone (17MT), a synthetic organic compound commonly found in sewage waters, can affect reproduction in aquatic animals, such as tilapia and yellow catfish. In the present study, male Gobiocypris rarus were exposed to 25, 50, and 100 ng/L of 17α-methyltestosterone (17MT) for 7 days. We first analyzed miRNA- and RNA-seq results to determine miRNA-target gene pairs and then developed miRNA-mRNA interactive networks after 17MT administration. Total weights, total lengths, and body lengths were not significantly different between the test groups and control groups. The paraffin slice method was applied to testes of G. rarus in the MT exposure and control groups. We found that there were more mature sperm (S) and fewer secondary spermatocytes (SSs) and spermatogonia (SGs) in the testes of control groups. As 17MT concentration increased, fewer and fewer mature sperm (S) were observed in the testes of male G. rarus. The results showed that FSH, 11-KT, and E2 were significantly higher in individuals exposed to 25 ng/L 17MT compared with the control groups. VTG, FSH, LH, 11-KT, and E2 were significantly lower in the 50 ng/L 17MT exposure groups compared to the control groups. VTG, FSH, LH, 11-KT, E2, and T were significantly lower in the groups exposed to 100 ng/L 17MT. High-throughput sequencing revealed 73,449 unigenes, 1205 known mature miRNAs, and 939 novel miRNAs in the gonads of G. rarus. With miRNA-seq, 49 (MT25-M vs. Con-M), 66 (MT50-M vs. Con-M), and 49 (MT100-M vs. Con-M) DEMs were identified in the treatment groups. Five mature miRNAs (miR-122-x, miR-574-x, miR-430-y, lin-4-x, and miR-7-y), as well as seven differentially expressed genes (soat2, inhbb, ihhb, gatm, faxdc2, ebp, and cyp1a1), which may be associated with testicular development, metabolism, apoptosis, and disease response, were assayed using qRT-PCR. Furthermore, miR-122-x (related to lipid metabolism), miR-430-y (embryonic development), lin-4-x (apoptosis), and miR-7-y (disease) were differentially expressed in the testes of 17MT-exposed G. rarus. This study highlights the role of miRNA-mRNA pairs in the regulation of testicular development and immune response to disease and will facilitate future studies on the miRNA-RNA-associated regulation of teleost reproduction.

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Section: Methodsmentioning

confidence: 99%

“…Previous studies have shown impaired testicular development in rare minnows ( Gobiocypris rarus ) exposed to 17MT (25–100 ng/L) [ 4 , 5 ]. Moreover, 302.5 ng/L 17MT disturbed gene expression in the hypothalamus–pituitary–gonadal axis of mummichog ( Fundulus heteroclitus ) [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of miRNA-mRNA Regulation in the Testes of Gobiocypris rarus following 17α-Methyltestosterone Exposure

Liu

Zhou

Yang

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…Then they were treated with different concentrations of MT (25, 50, and 100 ng/L named as MT25-F, MT50-F, and MT100-F, respectively, test groups) or with 0.0001% anhydrous ethanol (solvent control group) for 7 days. The concentration and exposure durations were determined according to our reports (Gao et al, 2014(Gao et al, , 2015 and the studies of Liang and Zha (2016) and Rutherford et al (2019). All experiments were performed in triplicate for the three treatment and one control groups.…”

Section: Experimental Animalsmentioning

confidence: 99%

“…Moreover, MT impaired the gonad development of C. auratus (Zheng et al, 2016) and the rare minnow species Gobiocypris rarus (25-100 ng/L) (Gao et al, 2014(Gao et al, , 2015Liang and Zha, 2016). MT (302.5 ng/L) disturbed the gene expression of the hypothalamuspituitary-gonadal axis in mummichog (Fundulus heteroclitus) (Rutherford et al, 2019). However, most studies, so far, have focused on gene expression without referring to the underlying microRNA (miRNA) regulation.…”

Section: Introductionmentioning

confidence: 99%

Integrated Analysis of mRNA- and miRNA-Seq in the Ovary of Rare Minnow Gobiocypris rarus in Response to 17α-Methyltestosterone

et al. 2021

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17α-Methyltestosterone (MT) is a synthetic androgen. The objective of this study was to explore the effects of exogenous MT on the growth and gonadal development of female rare minnow Gobiocypris rarus. Female G. rarus groups were exposed to 25–100 ng/L of MT for 7 days. After exposure for 7 days, the total weight and body length were significantly decreased in the 50-ng/L MT groups. The major oocytes in the ovaries of the control group were vitellogenic oocytes (Voc) and cortical alveolus stage oocytes (Coc). In the MT exposure groups, some fish had mature ovaries with a relatively lower proportion of mature oocytes, and the diameter of the perinucleolar oocytes (Poc) was decreased compared with those of the control group. Ovarian VTG, FSH, LH, 11-KT, E2, and T were significantly increased after exposure to 50 ng/L of MT for 7 days. Unigenes (73,449), 24 known mature microRNAs (miRNAs), and 897 novel miRNAs in the gonads of G. rarus were found using high-throughput sequencing. Six mature miRNAs (miR-19, miR-183, miR-203, miR-204, miR-205, and miR-96) as well as six differentially expressed genes (fabp3, mfap4, abca1, foxo3, tgfb1, and zfp36l1) that may be associated with ovarian development and innate immune response were assayed using qPCR. Furthermore, the miR-183 cluster and miR-203 were differentially expressed in MT-exposed ovaries of the different G. rarus groups. This study provides some information about the role of miRNA–mRNA pairs in the regulation of ovarian development and innate immune system, which will facilitate future studies of the miRNA–RNA-associated regulation of teleost reproduction.

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Physiological effects of 5α-dihydrotestosterone in male mummichog (Fundulus heteroclitus) are dose and time dependent

2019

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Comparison of steroidogenic gene expression in mummichog (Fundulus heteroclitus) testis tissue following exposure to aromatizable or non-aromatizable androgens

Cited by 4 publications

References 78 publications

Comparative Analysis of miRNA-mRNA Regulation in the Testes of Gobiocypris rarus following 17α-Methyltestosterone Exposure

Comparative Analysis of miRNA-mRNA Regulation in the Testes of Gobiocypris rarus following 17α-Methyltestosterone Exposure

Integrated Analysis of mRNA- and miRNA-Seq in the Ovary of Rare Minnow Gobiocypris rarus in Response to 17α-Methyltestosterone

Physiological effects of 5α-dihydrotestosterone in male mummichog (Fundulus heteroclitus) are dose and time dependent

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