Comparison of stem cells derived from periosteum and bone marrow of jaw bone and long bone in rabbit models

Park, Jun‐Beom; Bae, Sun-Sook; Lee, Phil-Woo; Lee, Won; Park, Yoon-Hee; Kim, Hee‐Sung; Lee, Kyoung Hwa; Kim, InSoo

doi:10.1007/s13770-012-0343-7

Cited by 13 publications

(8 citation statements)

References 20 publications

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“…Park et al compared the proliferation and multilineage differentiation of the cells from the periosteum and bone marrow of mandibular bone and the tibia using rabbit models. They found that the cells from the periosteum of the jaw bone had a higher ability than bone marrow-derived cells [25]. On the other hand, it was also reported that the number of proliferating cells as well as the frequency of colony-forming cells were significantly higher among dental pulp stem cells when compared with bone marrow stromal stem cells [26].…”

Section: Discussionmentioning

confidence: 98%

Calcified Nodule Formation <i>in Vitro</i> Using Rat Mandibular Incisor Pulp Cells

Ikenaga¹,

Yoshikawa²,

Miyamoto³

et al. 2018

JBiSE

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Section: Discussionmentioning

confidence: 98%

Calcified Nodule Formation <i>in Vitro</i> Using Rat Mandibular Incisor Pulp Cells

Ikenaga¹,

Yoshikawa²,

Miyamoto³

et al. 2018

JBiSE

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“…Intra‐oral periosteum is considered a readily available source of MSCs for clinical bone regeneration procedures because of the negligible inherent risk associated with harvesting, such as minimal donor‐site morbidity and esthetically compromising scar formation .…”

Section: Discussionmentioning

confidence: 99%

Localization and characterization of human palatal periosteum stem cells in serum‐free, xeno‐free medium for clinical use

Naung

Duncan

Silva

et al. 2019

European J Oral Sciences

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Harvesting, expanding, and re‐implanting osteogenic mesenchymal stem cells (MSCs) avoids the donor‐site morbidity associated with autogenous grafting from bone marrow. Mesenchymal stem cells sourced from the palatal periosteum could be an alternative to isolation of such cells using bone marrow aspiration procedures. For safe use in human therapy, MSCs should be expanded in culture medium that is free from animal or human‐derived serum. In this study we localized, quantified, and characterized MSCs from palatal periosteum cultured in serum‐free, xeno‐free Essential 8 medium. A portion of the palatal periosteum tissues from three patients were dual‐immunostained with MSC‐specific markers (CD105, CD90, and CD73). The remaining portions were expanded in culture, and the isolated MSCs were analyzed using flow cytometry and tri‐lineage differentiation. Palatal periosteum sections were found to contain CD105‐, CD90‐, and CD73‐positive cells. The cultured cells were 73.0 ± 6.7% (mean ± SD) positive for all three MSC‐specific markers and were without hematopoietic stem cell (HSC) markers 0.5 ± 0.3% (mean ± SD). Tri‐lineage differentiation analysis confirmed that palatal periosteum cells could become adipoblasts, chondroblasts, and osteoblasts. The results demonstrate that palatal‐derived MSCs could be detected in situ within small niches, and when expanded in serum‐free, xeno‐free medium represent a viable source of MSCs for clinical use.

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Section: Discussionmentioning

confidence: 99%

“…Stem cells can be obtained intraorally, including from the maxilla and mandible; this was suggested as a treatment option in tissue-engineering fields (13,14). The gingiva, periosteum and bone marrow from the maxillofacial region are considered favorable sources of stem cells, as stem cells can be harvested from the jaw bone under local anesthesia (13,15). Additionally, stem cells may be obtained intraorally, including from dental pulp and the periodontal ligament, but only a limited number of times; however, the gingiva is an easily accessible tissue source with wider availability (16).…”

Section: Discussionmentioning

confidence: 99%

Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

Lee

Yeo²,

Kim

et al. 2015

Biomedical Reports

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Abstract. Human mesenchymal stem cells have previously been isolated and characterized from the gingiva, and gingiva-derived stem cells have been applied for tissue engineering purposes. The present study was performed to generate size-controllable stem cell spheroids using concave microwells. Gingiva-derived stem cells were isolated, and the stem cells of 1x10 5 (group A) or 2x10 5 (group B) cells were seeded in polydimethylsiloxane-based, concave micromolds with 600 µm diameters. The morphology of the microspheres was viewed under an inverted microscope, and the changes in the diameter and cell viability were analyzed. The gingiva-derived stem cells formed spheroids in the concave microwells. The diameters of the spheroids were larger in group A compared to group B. No significant changes in shape or diameter were noted with increases in incubation time. Cell viability was higher in group B at each time point when compared with group A. Within the limits of the study, the size-controllable stem cell spheroids could be generated from gingival cells using microwells. The shape of the spheroids and their viability were clearly maintained during the experimental periods.

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Comparison of stem cells derived from periosteum and bone marrow of jaw bone and long bone in rabbit models

Cited by 13 publications

References 20 publications

Calcified Nodule Formation <i>in Vitro</i> Using Rat Mandibular Incisor Pulp Cells

Calcified Nodule Formation <i>in Vitro</i> Using Rat Mandibular Incisor Pulp Cells

Localization and characterization of human palatal periosteum stem cells in serum‐free, xeno‐free medium for clinical use

Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

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Comparison of stem cells derived from periosteum and bone marrow of jaw bone and long bone in rabbit models

Cited by 13 publications

References 20 publications

Calcified Nodule Formation &lt;i&gt;in Vitro&lt;/i&gt; Using Rat Mandibular Incisor Pulp Cells

Calcified Nodule Formation &lt;i&gt;in Vitro&lt;/i&gt; Using Rat Mandibular Incisor Pulp Cells

Localization and characterization of human palatal periosteum stem cells in serum‐free, xeno‐free medium for clinical use

Formation of size-controllable spheroids using gingiva-derived stem cells and concave microwells: Morphology and viability tests

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Calcified Nodule Formation <i>in Vitro</i> Using Rat Mandibular Incisor Pulp Cells

Calcified Nodule Formation <i>in Vitro</i> Using Rat Mandibular Incisor Pulp Cells