Comparison of standard most probable number method with three alternate tests for detection of bacteriological water quality indicators

Ramteke, Pramod W.

doi:10.1002/tox.2530100303

Cited by 5 publications

(2 citation statements)

References 15 publications

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“…While the Vibrio species were detected only in sample C. The common Enterobacteriaceae bacteria were observed in effluent only. The E. coli and coliform were prevalent in river water as well as effluent sample (Table IV) (Ramteke and Tewari, 2002;Ramteke, 1995;Ramteke et al, 1994).…”

Section: Discussionmentioning

confidence: 97%

Seasonal Bacteriological Analysis of Gola River Water Contaminated with Pulp Paper Mill Waste in Uttaranchal, India

Chandra

Singh

Raj

2006

Environ Monit Assess

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The seasonal physico-chemical and microbial quality of Gola river water has been analyzed after confluence of pulp paper mill waste. The study revealed that it has enhanced 20-30 times pollution load of BOD, COD, TDS, TSS, sulphate, chloride, sodium, nitrate, potassium, lignin and phenol after mixing of pulp paper mill waste with river water in all season. Further, it induced the bacterial growth by increasing most probable number value of E. coli was 1.57 x 10(4), 1.6 x 10(4), 1.37 x 10(4) and SPC count was 1.68 x 10(4), 1.64 x 10(4), 1.67 x 10(4)/100 ml during summer, monsoon, winter respectively. While the most probable number value in river water before mixing of pulp paper mill waste was 1.4 x 10(2), 1.82 x 10(2), 1.5 x 10(2) and SPC count was 2.8 x 10(3), 2.89 x 10(3), 2.78 x 10(3)/100 ml during summer, monsoon and winter respectively. This indicated from 88-114 fold increase in most probable number value of E. coli and 56.55-60.0 times increase in SPC count of river water after mixing of effluent in summer, monsoon and winter. Moreover, the most probable number value in effluent itself before mixing was 3.4 x 10(2), 3.3 x 10(2), 2.8 x 10(2) and SPC count was 6 x 10(4), 6.5 x 10(4), 6 x 10(4)/100 ml during summer, monsoon, winter, respectively. Furthermore, it was revealed that the seasonal variation also regulated the bacterial population dynamics as per the physico-chemical quality, in which E. coli was found highest at the rate of (5.9 x 10(4)), E. aerogenes (5.3 x 10(4)), P. aeruginosa (1.3 x 10(4)), S. aureus (3.2 x 10(3)), K. pneumoniae (2.6 x 10(4)), Enteritidis (1.1 x 10(4)) on monsoon season and V. cholerae (7.4 x 10(2)), V. vulnificus (9.2 x 10(2))/100 ml in river water when contaminated with pulp paper mill waste in monsoon season. Thus, the monsoon season showed presence of FC and TC indicated the thermo-tolerant and disease causing group of bacterial population in effluent and its sequence was observed as monsoon>summer>winter. This indicated the growth of many pathogenic and non-pathogenic bacteria for health hazards with contamination of pulp paper waste in aquatic ecosystem within the vicinity of pulp paper mill industry.

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Section: Discussionmentioning

confidence: 97%

Seasonal Bacteriological Analysis of Gola River Water Contaminated with Pulp Paper Mill Waste in Uttaranchal, India

Chandra

Singh

Raj

2006

Environ Monit Assess

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“…This procedure utilizing incubation at 45-C was found more sensitive than incubation at 37-C (Bolton, Crozier, & Williamson, 1996;Tewari, Ramteke, & Garg, 2003). As drinking water sources in rural areas are frequently contaminated with thermotolerant E. coli (Ramteke, 1995;Ramteke, Bhattacharjee, Pathak, & Kalra, 1992;Tewari et al, 2003), it was essential to elucidate their pathogenic serogroups and risk of dissemination of plasmids co-resistant to antibiotics and metal ions associated with these organisms.…”

Section: Introductionmentioning

confidence: 99%

Serogroups of Escherichia coli from Drinking Water

Ramteke

Tewari

2006

Environ Monit Assess

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Fifty seven isolates of thermotolerant E. coli were recovered from 188 drinking water sources, 45 (78.9%) were typable of which 15 (26.3%) were pathogenic serotypes. Pathogenic serogroup obtained were 04 (Uropathogenic E. coli, UPEC), 025 (Enterotoxigenic E. coli, ETEC), 086 (Enteropathogenic E. coli, EPEC), 0103 (Shiga-toxin producing E. coli, STEC), 0157 (Shiga-toxin producing E. coli, STEC), 08 (Enterotoxigenic E. coli, ETEC) and 0113 (Shiga-toxin producing E. coli, STEC). All the pathogenic serotypes showed resistance to bacitracin and multiple heavy metal ions. Resistance to streptomycin and cotrimazole was detected in two strains whereas resistance to cephaloridine, polymixin-B and ampicillin was detected in one strain each. Transfer of resistances to drugs and metallic ions was observed in 9 out of 12 strains studied. Resistances to bacitracin were transferred in all nine strains. Among heavy metals resistance to As(3+) followed by Cr(6+) were transferred more frequently.

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