Comparison of Spectrophotometric and Spectrophotofluorometric Methods for the Determination of Malonaldehyde.

Sawîckî, Eugene; Stanley, T. W.; Johnson, Henry W. B.

doi:10.1021/ac60195a026

Cited by 65 publications

(17 citation statements)

References 22 publications

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“…Lipid peroxidation, measured as malondialdehyde production in liver samples, was determined by the method of Sawicki et al (1963) employing malondialdehyde as standard.…”

Section: Lipid Peroxidationmentioning

confidence: 99%

Taurine: Protective properties against ethanol-induced hepatic steatosis and lipid peroxidation during chronic ethanol consumption in rats

et al. 1998

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Alcohol was administered chronically to female Sprague Dawley rats in a nutritionally adequate totally liquid diet for 28 days. This resulted in hepatic steatosis and lipid peroxidation. Taurine, when co-administered with alcohol, reduced the hepatic steatosis and completely prevented lipid peroxidation. The protective properties of taurine in preventing fatty liver were also demonstrated histologically. Although alcohol was found not to affect the urinary excretion of taurine (a non-invasive marker of liver damage), levels of serum and liver taurine were markedly raised in animals receiving alcohol + taurine compared to animals given taurine alone. The ethanol-inducible form of cytochrome P-450 (CYP2E1) was significantly induced by alcohol; the activity was significantly lower than controls and barely detectable in animals fed the liquid alcohol diet containing taurine. In addition, alcohol significantly increased homocysteine excretion into urine throughout the 28 day period of ethanol administration; however, taurine did not prevent this increase. There was evidence of slight cholestasis in animals treated with alcohol and alcohol + taurine, as indicated by raised serum bile acids and alkaline phosphatase (ALP). The protective effects of taurine were attributed to the potential of bile acids, especially taurine conjugated bile acids (taurocholic acid) to inhibit the activity of some microsomal enzymes (CYP2E1). These in vivo findings demonstrate for the first time that hepatic steatosis and lipid peroxidation, occurring as a result of chronic alcohol consumption, can be ameliorated by administration of taurine to rats.

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“…Lipid peroxidation, measured as malondialdehyde production in liver samples, was determined by the method of Sawicki et al (1963) employing malondialdehyde as standard.…”

Section: Lipid Peroxidationmentioning

confidence: 99%

Taurine: Protective properties against ethanol-induced hepatic steatosis and lipid peroxidation during chronic ethanol consumption in rats

et al. 1998

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“…The method has been extensively reviewed and discussed by Barber & Bernheim (3) and Slater (4). Several investigators have attempted to improve the reliability or reproducibility of the test while analyzing biological material (5)(6)(7)(8)(9)(10). Failure to detect TBA-rea c ting material in tissue extracts is not an indication of the absence of lipid peroxidation.…”

Section: Measurement Of Lipid Peroxida Nonmentioning

confidence: 99%

Chemicals, Drugs, and Lipid Peroxidation

Plaa¹,

Witschi²

1976

Annu. Rev. Pharmacol. Toxicol.

490

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“…The low molecular weight bifunctional aldehydes, malonaldehyde and 4-hydroxynonenal, have been extensively investigated for their reactivity with various amines, amino acids and proteins, with which they yield relatively stable covalently bound products. Specifically, malonaldehyde has been shown to undergo 1,4-addition with amino acids to form the eneamines (2), N-substituted 3-iminopropenals (3) and N,N′-disubstituted 1-amino-3-iminopropenes (4). Malonaldehyde also reacts with the amino groups in proteins (4,5) as well as with deoxynucleosides in vitro to produce a variety of adducts (6).…”

mentioning

confidence: 99%

Preparation of schiff base adducts of phosphatidylcholine core aldehydes and aminophospholipids, amino acids, and myoglobin

Ravandi

Kuksis

Shaikh

et al. 1997

Lipids

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We have prepared Schiff base adducts of the core aldehydes of phosphatidylcholine and aminophospholipids, free amino acids, and myoglobin. The Schiff bases of the ethanolamine and serine glycerophospholipids were obtained by reacting sn-1-palmitoyl(stearoyl)-2-[9-oxo]nonanoyl-glycerophosphocholine (PC-Ald) with a twofold excess of the aminophospholipid in chloroform/methanol 2:1 (vol/vol) for 18 h at room temperature. The Schiff bases of the amino acids and myoglobin were obtained by reacting the aldehyde with an excess of isoleucine, valine, lysine, methyl ester lysine and myoglobin in aqueous methanol for 18 h at room temperature. Prior to isolation, the Schiff bases were reduced with sodium cyanoborohydride in methanol for 30 min at 4 degrees C. The reaction products were characterized by normal-phase high-performance liquid chromatography and on-line mass spectrometry with electrospray ionization. The amino acids and aminophospholipids yielded single adducts. A double adduct was obtained for myoglobin, which theoretically could have accepted up to 23 PC-Ald groups. The yields of the products ranged from 12 to 44% for the aminophospholipids and from 15-57% for the amino acids, while the Schiff base of the myoglobin was estimated at 5% level. The new compounds are used as reference standards for the detection of high molecular weight Schiff bases in lipid extracts of natural products.

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Comparison of Spectrophotometric and Spectrophotofluorometric Methods for the Determination of Malonaldehyde.

Abstract: Ten new methods for the determination of malonaldehyde are compared with the thiobarbituric acid

Cited by 65 publications

References 22 publications

Taurine: Protective properties against ethanol-induced hepatic steatosis and lipid peroxidation during chronic ethanol consumption in rats

Taurine: Protective properties against ethanol-induced hepatic steatosis and lipid peroxidation during chronic ethanol consumption in rats

Chemicals, Drugs, and Lipid Peroxidation

Preparation of schiff base adducts of phosphatidylcholine core aldehydes and aminophospholipids, amino acids, and myoglobin

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