Comparison of spectrophotometric and electrophoretic measurements of glutamate dehydrogenase activity during mycelial growth and carpophore development in the basidiomycete Coprinus cinereus

Jirjis, Raída

doi:10.1016/0378-1097(79)90111-3

Cited by 1 publication

(4 citation statements)

References 5 publications

(8 reference statements)

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“…The strains used here were the monokaryons HI (mating type A^Bj), BC9/6,6 (A^Be) and ZBw601/40,40 (A^oB^o) together with the dikaryons BC9/6,6 + Hl, BC9/6,6 + ZBw601/40,40 (= dikaryon RAl of Moore and Jirjis, 1976) and the dikaryon 601 which was isolated in Czechoslovakia by J. Necasek and from which monokaryon ZBw601/40,40 was derived (Moore and Stewart, 1972). Vegetative cultures and carpophores were grown as described by Jirjis and Moore (1979).…”

Section: Organismmentioning

confidence: 99%

“…Enzyme activity in the gels was detected using assay mixtures modified from those reported by Cole, Blondin and Temple (1968) and Shaw and Prasad (1970). Protein contents of the extracts were measured by the method of Lowry et al (1951) and the specific activities of individual enzyme bands calculated with the aid of densitometric records as described earlier (Jirjis and Moore, 1979). It is important to emphasize that throughout the preparative stages (extraction, desalting and electrophoresis) the temperature of the material did not exceed 4°C.…”

Section: Electrophoresismentioning

confidence: 99%

“…It is important to emphasize that throughout the preparative stages (extraction, desalting and electrophoresis) the temperature of the material did not exceed 4°C. Glutamate dehydrogenase activity was assayed in each of the different types of material described and both GDH enzymes were always found to migrate as single bands (Jirjis and Moore, 1979).…”

Section: Electrophoresismentioning

confidence: 99%

“…Cell-free extracts were prepared, desalted, and subjected to electrophoresis using the techniques described by Jirjis and Moore (1979) except that in some experiments described below the acrylamide concentration was varied. For general proteins gels were stained with Gurr's Naphthalene Blue Black lOB.…”

Section: Electrophoresismentioning

confidence: 99%

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ELECTROPHORETIC STUDIES OF CARPOPHORE DEVELOPMENT IN THE BASIDIOMYCETE COPRINUS CINEREUS

Moore

Jirjis

1981

New Phytologist

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SUMMARYAcrylamide gel electrophoresis was used to resolve buffer-soluble proteins and enzymes extracted from tissues of Copritius cinereus. The electrophoretograms of proteins of two related but compatible monokaryons differed from one another, and both differed from the electrophoretogram of the dikaryon made by crossing the monokaryons together. The electrophoretogram of the youngest carpophore primordia was markedly different from that of the dikaryon on which they were produced, and the protein spectrum of both cap and stipe altered as the tissues matured. The protein electrophoretic pattern observed in the cap was always different from that of the stipe on which the cap was borne. Hov/ever, when carpophores of quite different dikaryons were compared it was found that differences between the strains were so considerable that it was not possible to identify features common to a particular tissue or structure. Variation was also found in respect of isozyme patterns even though only two quite closely related monokaryons were compared.In the case of two enzyme activities (malate dehydrogenase and NADPH-dehydrogenase) the monokaryons exhibited different numbers of isozymes, and in a further three (alcohol dehydrogenase, NADH-dehydrogenase and esterase) there were clear differences in the way in which the isozyme pattern in each monokaryon related to the period of growth. The dikaryon was not associated with any predictable change in the isozyme pattern, but in all cases the dikaryon was in some way different from the parental monokaryons. Further differences were observed in carpophore tissues: in all cases the carpophore differed from its parental dikaryon. In most cases this difference was expressed as a distinctive developmental pattern of isozymes which were also detectable in mycelium; and in this the cap was often quite different from the stipe. However, for alcohol dehydrogenase and esterase, extracts of carpophore tissues possessed isozymes not detectable in extracts of mycelia.

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Section: Organismmentioning

confidence: 99%

Section: Electrophoresismentioning

confidence: 99%

Section: Electrophoresismentioning

confidence: 99%

Section: Electrophoresismentioning

confidence: 99%

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