Comparison of soymilk, powdered milk, Hank’s balanced salt solution and tap water on periodontal ligament cell survival

Abstract

The purpose of this study was to evaluate the ability of soymilk, powdered milk, and Hank's balanced salt solution (HBSS) to maintain human periodontal ligament (PDL) cell viability in vitro. PDL cells were obtained from extracted healthy third molars and cultured in Dulbecco's modified Eagles medium (DMEM). The cultures were exposed for 1, 2, 4, and 8 h to experimental solutions (tap water served as negative control and DMEM as positive control) at 37°C. The viable cells were then counted using the trypan blue exclusion technique. Data were analyzed by using one-way anova, post hoc Scheffe and two-way anova test. Statistical analysis showed that HBSS, powdered baby formula, and soymilk maintain cell viability equally well in different periods of times. Tap water cannot keep cells viable as well as other solutions. Soymilk and powdered baby formula can be recommended as suitable storage media for avulsed teeth for up to 8 h.

Meios De Armazenamento Para Dentes Avulsionados - Uma Revisão De Literatura

“…No entanto o artigo recomenda a utilização do HBSS, devido a indisponibilidade do meio de transporte contendo amora 36 . A água de torneira demonstrou-se inapropriada como meio de armazenamento devido sua hipotonicidade, levando a rápida lise celular 16,27 . Meios como a saliva foram mais efetivos que a água de torneira, mas também demonstraram potencial de contaminação bacteriana 26 .…”

“…O meio que apresentar uma osmolalidade acima de 230 mOsm kg -1 é considerado uma [15][16][17] . Save-A-Tooth é comercializado em um kit que contém um pequeno cesto em que o dente avulsionado é suspenso e submerso em HBSS.…”

“…Leite O leite apresenta uma osmolalidade e um pH adequados, além de substâncias nutricionais para manter a viabilidade das células do ligamento periodontal 9,16 . A pasteurização, processo que o leite passa em sua produção, faz com que ele contenha poucas bactérias, diminuindo a contaminação do dente 9,36 .…”

Effect of different storage media on root dentine composition and viability of fibroblasts evaluated by several assay methods

“…Recently, soya milk (SM) was proposed (Moazami et al . , Moura et al . ) as an alternative storage medium.…”

Egg White-Milk Mixture as a Novel Biological Storage Media for Avulsed teeth Periodontal Ligament Cells: An In-Vitro Study

“…All rabbits were anaesthetized according to Cornell University Institutional Animal Care and Use Committee rabbit anaesthesia protocol [28]. Using xylazine (Xyla-Ject injectable sol.…”