Comparison of soybean and pea ENOD40 cDNA clones representing genes expressed during both early and late stages of nodule development

Matvienko, M.; Sande, K. van de; Yang, Wei‐Cai; Kammen, A. van; Bisseling, Ton; Franssen, Henk

doi:10.1007/bf00039559

Cited by 53 publications

(34 citation statements)

References 10 publications

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“…Nodulins are plant-encoded proteins that are expressed during nodule development. ENOD40 is one of the earliest nodulins to be expressed upon Rhizobium inoculation, and has been cloned from a number of legumes (Kouchi and Hata, 1993;Yang et al, 1993;Asad et al, 1994;Crespi et al, 1994;Matvienko et al, 1994;Vijn et al, 1995b;Papadopoulou et al, 1996) as well as one nonlegume, tobacco (van de Sande et al, 1996). In alfalfa (Medicago sativa) ENOD40 transcripts have been detected in dividing cells or cells that are competent to divide (Asad et al, 1994;Crespi et al, 1994).…”

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confidence: 99%

Studying Early Nodulin GeneENOD40Expression and Induction by Nodulation Factor and Cytokinin in Transgenic Alfalfa1

1998

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ENOD40, an early nodulin gene, is expressed following inoculation with Rhizobium meliloti or by adding R. meliloti-produced nodulation (Nod) factors or the plant hormone cytokinin to uninoculated roots. We isolated two MsENOD40 clones, designated MsENOD40-1 and MsENOD40-2, with distinct promoters from an alfalfa (Medicago sativa cv Chief) genomic library. The promoters were fused to the reporter gene uidA (gus), and the constructs were introduced into alfalfa. We observed that the MsENOD40-1 construct was expressed almost exclusively under symbiotic conditions. The MsENOD40-2 construct was transcribed under both symbiotic and nonsymbiotic conditions and in nonnodular and nodular tissues. Both MsENOD40 promoter-gus constructs were similarly expressed as nodules developed, and both were expressed in roots treated with 6-benzylaminopurine or purified Nod factor. However, no blue color was detected in nodule-like structures induced by the auxin transport inhibitor N-1-(naphthyl)phthalamic acid on roots of plants containing the MsENOD40-1 promoter construct, whereas pseudonodules from plants containing the MsENOD40-2 promoter construct stained blue. A 616-bp region at the distal 5 end of the promoter is important for proper spatial expression of MsENOD40 in nodules and also for Nod-factor and cytokinin-induced expression.

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confidence: 99%

Studying Early Nodulin GeneENOD40Expression and Induction by Nodulation Factor and Cytokinin in Transgenic Alfalfa1

1998

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“…Sparkle root nodule RNA, was kindly provided by G. Coruzzi [33] and seven early nodulin cDNA clones were isolated by differential screening [30]. Previously, we have described the characterization of six clones, namely pPsENOD2, pPsENOD3, pPsENOD5, pPsENOD12, pPsENOD14 and pPsENOD40 [22,29,30,34]. Here, we present the characterization of pPsENOD7.…”

Section: Isolation Of Ppsenod7mentioning

confidence: 99%

“…In pea six early nodulin genes have been described, such as PsENOD12 [29] and PsENOD40 [22], and several late nodulin genes, such as glutamine synthetase (GS) [33], leghaemoglobin (Lb) [23] and PsNOD6 [16].…”

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confidence: 99%

The pea early nodulin gene PsENOD7 maps in the region of linkage group I containing sym2 and leghaemoglobin

et al. 1996

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The early nodulin gene, PsENOD7, is expressed in pea root nodules induced by Rhizobium leguminosarum bv. viciae, but not in other plant organs. In situ hybridization showed that this gene is transcribed during nodule maturation in the infected cells of the proximal part of the prefixation zone II. At the transition of zone II into interzone II-III, the level of PsENOD7 mRNA drops markedly. PsENOD7 has no significant homology to other genes. RFLP mapping studies have shown that PsENOD7 is located in linkage group I between the leghaemoglobin genes and sym2.Rhizobium leguminosarum bv. viciae induces the formation of nitrogen fixing root nodules on the roots ofPisum sativum (pea). By mutagenesis and genetic studies several plant genes essential for normal nodule development have been identified and these genes have been named sym genes. In pea about 30 different sym genes have been described [7,18,19,26]. The sym genes are distributed randomly on the seven linkage groups of pea [19], but several sym genes, namely sym2, sym5, syml9 and nod3, are clustered on linkage group I, near the major leghaemoglobin (Lb) locus [32, 381.The different stages of legume nodule development are accompanied by the expression of plant genes, the so-called nodulin genes. These genes, that are only expressed during nodule development, have been divided into early and late nodulin genes; the early nodulin genes are expressed before the bacteria start to fix nitrogen, whereasThe nucleotide sequence data reported will appear in the EMBL, GenBank and DDBJ Nucleotide Sequence Databases under the accession number X93172.

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“…For example, Turcich and Mascarenhas (1994) have described a family of repetitive sequences related to retroelement repeat sequences that produce transcripts of heterogeneous lengths in a variety of plant organs, but it is not known whether these transcripts have a biological function. There are also reports of a gene with an unusual structure, the early nodulin fNOD40 gene from soybean (Yang et al, 1993), pea (Matvienko et al, 1994), alfalfa (Asad et al, 1994), and Medicago (Crespi et al, 1994), which has been proposed by some to function in nodule development as an RNA because it has limited protein-coding capacity and can potentially encode only a short peptide. Although it is formally possible that any function performed by the SLAz gene is mediated by its RNA transcripts, our working hypothesis is that one of the ORFs contained within the spliced SLA2 transcript is translated to produce a highly basic pollen coat protein of 7.5 or 10 kD.…”

Section: Hindrnmentioning

confidence: 99%

An anther-specific gene encoded by an S locus haplotype of Brassica produces complementary and differentially regulated transcripts.

Boyes

Nasrallah

1995

Plant Cell

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The self-incompatibility locus of Brassica consists of a coadapted gene complex that contains at least two genes required for the recognition and inhibition of pollen by the stigma when self-pollinated. Here, we report the identification of a third S locus-linked gene from the Sz haplotype of Brassica oleracea. This gene, which we designated SLA (for S Locus Anther), is a nove1 gene with an unusual structure. SLA is transcribed from two promoters to produce two complementary anther-specific transcripts, one spliced and the other unspliced, that accumulate in an antiparallel manner in developing microspores and anthers. The sequence of the spliced transcript showed the presence of two open reading frames that predict proteins of 10 and 7.5 kD. Neither transcript was produced in a self-compatible 8. napus strain carrying an Srlike haplotype, indicating that the SLA gene in this strain is nonfunctional. Interestingly, sequences related to SLA were not detected in DNA or RNA from plants carrying S haplotypes other than Sz. The haplotype specificity of SLA, its anther-specific expression, and its physical linkage to the S locus are properties expected for a gene that encodes a determinant of S2 specificity in pollen.

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Comparison of soybean and pea ENOD40 cDNA clones representing genes expressed during both early and late stages of nodule development

Cited by 53 publications

References 10 publications

Studying Early Nodulin GeneENOD40Expression and Induction by Nodulation Factor and Cytokinin in Transgenic Alfalfa1

Studying Early Nodulin GeneENOD40Expression and Induction by Nodulation Factor and Cytokinin in Transgenic Alfalfa1

The pea early nodulin gene PsENOD7 maps in the region of linkage group I containing sym2 and leghaemoglobin

An anther-specific gene encoded by an S locus haplotype of Brassica produces complementary and differentially regulated transcripts.

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