Comparison of some biochemical tests in different blood collection tubes in hemodialysis patients

Kösem, Arzu; Topçuoğlu, Canan; Sezer, Sevilay; Cevher, Şimal Köksal; Yenigun, Ezgi Coskun; Dede, Fatih; Turhan, Turan

doi:10.1515/tjb-2018-0341

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…Analyte results comparison given from both tubes were in line with our expectations and in agreement with previous examinations comparing serum and plasma specimen results (9,14-16 AST, LD and glucose in BD Barricor compared to serum samples (9). Difference in K concentration between plasma and serum tubes was observed in all mentioned studies (bias ranged from -4.9 up to -7.1%) and it was similar to the findings in our study (9,(14)(15)(16). Our study, together with all mentioned studies, found a higher bias for K than the one stated for desirability in both the EFLM Biological Variation Database or Westgard's database (11,12).…”

Section: Discussionsupporting

confidence: 92%

“…Analyte results comparison given from both tubes were in line with our expectations and in agreement with previous examinations comparing serum and plasma specimen results ( 9 , 14 - 16 ). Kosem et al compared eight biochemistry tests from BD RST and BD Barricor tubes with the BD Serum Separator Tubes (SST) as reference tubes ( 16 ). Their study was performed in haemodialysis patients.…”

Section: Discussionsupporting

confidence: 92%

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Test results comparison and sample stability study

Mandić

Lukić

et al. 2020

Biochem. med. (Online)

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Introduction: The aim was to evaluate the BD Barricor tubes by comparison with the BD Rapid Serum Tubes (RST) through measuring 25 analytes and monitoring sample stability after 24 hours and 7 days. Materials and methods: Samples of 52 patients from different hospital departments were examined. Blood was collected in BD RST and BD Barricor tubes (Becton, Dickinson and Company, Franklin Lakes, USA). Analytes were measured by Beckman Coulter AU 480 (Beckman Coulter, Brea, USA), Dimension EXL (Siemens Healthcare Diagnostics, Newark, USA) and ARCHITECT i2000SR (Abbott Diagnostics, Lake Forest, USA). Between-tube comparison for each analyte was performed, along with testing analyte stability after storing samples at 4 °C. Results: BD Barricor tubes showed unacceptable bias compared to BD RST tubes for potassium (K) (- 4.5%) and total protein (4.4%). Analyte stability after 24 hours was acceptable in both tested tubes for most of analytes, except for glucose, aspartate aminotransferase (AST) and lactate dehydrogenase (LD) in BD Barricor and free triiodothyronine in BD RST sample tubes. Analyte stability after 7 days was unacceptable for sodium, K, calcium, creatine kinase isoenzyme MB, AST, LD and troponin I in both samples; additionally for glucose, alkaline phosphatase and albumin in BD Barricor. Conclusion: All analytes, except K and total protein, can be measured interchangeably in BD RST and BD Barricor tubes, applying the same reference intervals. For most of the analytes, sample re-analysis can be performed in both tubes after 24 hours and 7 days, although BD RST tubes show better 7-day analytes stability over BD Barricor tubes.

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Section: Discussionsupporting

confidence: 92%

“…Analyte results comparison given from both tubes were in line with our expectations and in agreement with previous examinations comparing serum and plasma specimen results ( 9 , 14 - 16 ). Kosem et al compared eight biochemistry tests from BD RST and BD Barricor tubes with the BD Serum Separator Tubes (SST) as reference tubes ( 16 ). Their study was performed in haemodialysis patients.…”

Section: Discussionsupporting

confidence: 92%

Test results comparison and sample stability study

Mandić

Lukić

et al. 2020

Biochem. med. (Online)

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“…The systematic error and proportional difference observed for the Ca, GGT, and INS analytes were not clinically significant, as all three tests remained within the BV %TEa limits (Table 1). Kösem et al 23 similarly interpreted their findings on parathormone. According to Ercan et al 14 the confidence interval for the CRP intercept did not contain a value of 0.…”

Section: Discussionmentioning

confidence: 76%

The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory

ZENGİ

2023

J Health Sci Med /JHSM /jhsm

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Aims: To assess the viability of replacing serum samples with plasma samples in various clinical chemistry and immunoassay tests and to examine the implications of turnaround time (TAT) and sample quality during the transition process. Methods: We compared the results of 27 paired clinical chemistry and 13 immunoassay tests from samples obtained using gel separator serum and gel separator lithium heparinized plasma (LIH) tubes. We used regression analysis, bias values, and Bland-Altman plots to compare the performance of serum and LIH tubes in various clinical chemistry and immunoassay tests. We collected and evaluated sample aspiration errors, hemolysis index values, and TAT data from the laboratory information system before and after switching to plasma in our study. Results: Most tests showed no significant difference between the serum and LIH. However, for some analytes, total error (TE) values exceeded the total allowable error (TEa) limits derived from the biological variation database. Notably, insulin TE value did not exceed TEa, but it consumed near all its error budget. Consequently, we determined the alternative allowable error limits for some tests and found that plasma tubes could be used instead of serum tubes for most tests, except for lactate dehydrogenase (LDH). Plasma tubes improved the sample quality, reduced the incidence of aspiration errors, and decreased TAT in the emergency laboratory. We observed significant reductions in TAT after switching to plasma tubes. Conclusion: Our study showed that LIH tubes can replace serum tubes in most clinical chemistry and immunoassay tests. Using LIH tubes in clinical laboratories can improve healthcare quality and reduce the workload of the laboratory staff.

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“…Produsen telah memproduksi berbagai tabung yang menyajikan pemisahan berkualitas tinggi antara serum atau plasma dan sel untuk mengurangi TAT dan mengurangi cellular intent (Kösem et al, 2020). Penelitian tentang penggunaan vacutainer serum separator ini juga sesuai dengan penelitian yang dilakukan oleh (Wei et al, 2010)…”

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Vacutainer serum separator sebagai alternatif tabung penampung darah pada pemeriksaan kadar ureum

Setiawan¹,

Nugraheni²,

Rahayu³

2021

THE JAMMILT

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Serum using is preferred for urea level because it does not use anticoagulants which can interfere with activity and reaction to the results. The tubes that are widely used to collect blood into serum are vacutainer serum separator and vacutainer plain.This researche aims to determine the degree of agreement s between vacutainer serum separator and vacutainer plain usage on serum urea level result.This research was cross sectional design and hold on October 2020 with subject were taken from thirty blood samples of health analyst students which taken randomly and had no history of disease or kidney function disorder. Each student was taken 6 ml of blood drawn using a venoject with each vacutainer containing 3 ml, so we had 60 data. The data were analyzed by descriptively and inferentially using the Interclass Correlation Coefficient (ICC) statistical test. From the descriptive analysis, the difference in mean levels was 0.35 mg/dL and the ICC statistical test resulted in a degree of agreement 0.745. The data were analyzed by descriptively and inferentially using the Interclass Correlation Coefficient (ICC) statistical test. From the descriptive analysis, the difference in mean levels was 0.35 mg/dL and the ICC statistical test resulted in a degree of agreement was 0.745. The calculation of the average working time between the vacutainer serum separator and the vacutainer plain was 4 minutes 38 seconds and 35 minutes 58 seconds. The analysis concluded that the vacutainer serum separator and the vacutainer plain could be used as an alternative of blood collecting tubes for urea level testing which proved to be no significant difference in the results from this research. Keywords : Urea level, Vacutainer Serum Separator, Vacutainer Plain

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Comparison of some biochemical tests in different blood collection tubes in hemodialysis patients

Cited by 7 publications

References 16 publications

Test results comparison and sample stability study

Test results comparison and sample stability study

The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory

Vacutainer serum separator sebagai alternatif tabung penampung darah pada pemeriksaan kadar ureum

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