Comparison of Soil Higher Fungal Communities between Dead and Living <i>Abies koreana</i> in Mt. Halla, the Republic of Korea

Kim, Chang Sun; Jo, Jong Won; Lee, Hyen; Kwag, Young-Nam; Cho, Sung Eun; Oh, Seung Hwan

doi:10.1080/12298093.2020.1811193

Cited by 5 publications

(6 citation statements)

References 37 publications

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“…Applying metagenomics to analyze microbial community from various environments was also conducted in Korea [20][21][22][23][24][25][26]. Of which, NGS platform of Roche 454 was applied to identify airborne fungal community in Seoul [21], while Illumina MiSeq platform was performed to compare soil higher fungal communities associated with dead and living Korean fir (Abies koreana) in Jeju island [26]. However, using metagenomics to analyze endophytic fungal communities in forest trees is still limited.…”

Section: Introductionmentioning

confidence: 99%

Fungal Community Analyses of Endophytic Fungi from Two Oak Species, Quercus mongolica and Quercus serrata, in Korea

2021

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Fungal endophytes have been recorded in various plant species with a richness of diversity, and their presence plays an essential role in host plant protection against biotic and abiotic stresses. This study applied the Illumina MiSeq sequencing platform based on the amplification of fungal ribosomal ITS2 region to analyze fungal endophytic communities of two oak species (Quercus mongolica and Q. serrata) with different oak wilt disease susceptibilities in Korea. The results showed a total of 230,768 sequencing reads were obtained and clustered at a 97% similarity threshold into 709 operational taxonomic units (OTUs). The OTUs of Q. serrata were higher than that of Q. mongolica with the number of 617 OTUs and 512 OTUs, respectively. Shannon index also showed that Q. serrata had a significantly higher level of fungal diversity than Q. mongolica. Total of OTUs were assigned into 5 fungal phyla, 17 classes, 60 orders, 133 families, 195 genera, and 280 species. Ascomycota was the dominant phylum with 75.11% relative abundance, followed by Basidiomycota with 5.28%. Leptosillia, Aureobasidium and Acanthostigma were the most abundant genera detected in Q. serrata with the average relative abundance of 2.85, 2.76, and 2.19%, respectively. On the other hand, Peltaster, Cladosporium and Monochaetia were the most common genera detected in Q. mongolica with the average relative abundance of 4.83, 3.03, and 2.87%, respectively. Our results indicated that fungal endophytic communities were significantly different between two oak species and these differences could influence responses of host trees to oak wilt disease caused by Raffaelea quercus-mongolicae.

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Section: Introductionmentioning

confidence: 99%

Fungal Community Analyses of Endophytic Fungi from Two Oak Species, Quercus mongolica and Quercus serrata, in Korea

2021

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“…Members of the Massilia genus were shown in a previous study to have a reduced population in suppressive soils of fungal pathogens and to be negatively correlated with important plant-growth-promoting microbes such as Rhizobium, Paenibacillus , and Streptomyces [ 56 ]. Fungal pathogens are known to be important factors affecting the forest trees’ health status and a clear distinction in the composition was previously reported in a comparative study [ 21 ]. A previous study also reported the effect of soil-borne fungi on the overwintering survival of A. koreana seeds.…”

Section: Discussionmentioning

confidence: 99%

“…Hence, the rhizosphere microbiome should be considered as a major aspect in studying forest ecosystems and among the factors contributing to the status of trees. A previous recent study has investigated the community structure of higher fungi between dead and living Korean fir trees and indicated the significant differences in the composition [ 21 ]. However, the bacterial rhizosphere composition and links to tree status remain unclear.…”

Section: Introductionmentioning

confidence: 99%

Dysbiosis in the Rhizosphere Microbiome of Standing Dead Korean Fir (Abies koreana)

Han

Mannaa

Jeon

et al. 2022

Plants

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The Korean fir (Abies koreana), a native coniferous tree species mainly found on Mt. Halla in Jeju, South Korea, is suffering from continuous population decline and has been declared an endangered species. Research efforts have focused on the possible abiotic causes behind this worrying decline. However, the potential link between tree vitality and the rhizosphere microbiome remains unclear. In this study, a comparative metagenomic 16S rRNA sequence analysis was used to investigate the composition of the rhizosphere microbiota of samples collected from healthy and die-back-affected trees on Mt. Halla. The results indicated a significant reduction in the richness and diversity of microbiota in the rhizosphere of die-back-affected trees. Moreover, the relative abundance of Proteobacteria, Actinobacteria, and Bacteroidetes were significantly higher in healthy trees than in standing dead trees. Many bacterial genera were significantly more abundant in the rhizosphere of healthy trees, including those known for promoting plant growth and tolerance to biotic and abiotic stresses (e.g., Bradyrhizobium, Rhizomicrobium, Caulobacter, Nitrosospira, Rhizobacter, Paraburkholderia, Rhizobium, Devosia, Caballeronia, Niveispirillum, Dyella, Herbaspirillum, Frankia, Streptomyces, Actinoallomurus, Lysobacter, Luteibacter, Mucilaginibacter, and Variovorax). To our knowledge, this is the first report on rhizosphere bacterial microbiome dysbiosis in die-back-affected Korean fir trees, suggesting that the influence of rhizosphere microbiota should be considered to save this endangered species by investigating possible intervention strategies in future work.

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“…Kim et al. [ 34 ] analyzed the diversity of Ascomycota and Basidiomycota in the rhizosphere soil of A. koreana in Mt. Halla.…”

Section: Discussionmentioning

confidence: 99%

Diversity and community structure of ectomycorrhizal mycorrhizal fungi in roots and rhizosphere soil of Abies koreana and Taxus cuspidata in Mt. Halla

Lee

Eom

2022

Mycobiology

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In this study, the roots and rhizosphere soil of Abies koreana and Taxus cuspidata were collected from sites at two different altitudes on Mt. Halla. Ectomycorrhizal fungi (EMF) were identified by Illumina MiSeq sequencing. The proportion of EMF from the roots was 89% in A. koreana and 69% in T. cuspidata . Among EMF in rhizosphere soils, the genus Russula was the most abundant in roots of A. koreana ( p < 0.05). The altitude did not affect the biodiversity of EMF communities but influenced fungal community composition. However, the host plants had the most significant effect on EMF communities. The result of the EMF community analysis showed that even if the EMF were isolated from the same altitudes, the EMF communities differed according to the host plant. The community similarity index of EMF in the roots of A. koreana was higher than that of T. cuspidata ( p < 0.05). The results show that both altitude and host plants influenced the structure of EMF communities. Conifers inhabiting harsh sub-alpine environments rely strongly on symbiotic relationships with EMF. A. koreana is an endangered species with a higher host specificity of EMF and climate change vulnerability than T. cuspidata . This study provides insights into the EMF communities, which are symbionts of A. koreana, and our critical findings may be used to restore A. koreana .

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Comparison of Soil Higher Fungal Communities between Dead and Living Abies koreana in Mt. Halla, the Republic of Korea

Cited by 5 publications

References 37 publications

Fungal Community Analyses of Endophytic Fungi from Two Oak Species, Quercus mongolica and Quercus serrata, in Korea

Fungal Community Analyses of Endophytic Fungi from Two Oak Species, Quercus mongolica and Quercus serrata, in Korea

Dysbiosis in the Rhizosphere Microbiome of Standing Dead Korean Fir (Abies koreana)

Diversity and community structure of ectomycorrhizal mycorrhizal fungi in roots and rhizosphere soil of Abies koreana and Taxus cuspidata in Mt. Halla

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