Comparison of Six Real-Time PCR Assays for Qualitative Detection of Cytomegalovirus in Clinical Specimens

Binnicker, M. J.; Espy, M.

doi:10.1128/jcm.02005-13

Cited by 25 publications

(12 citation statements)

References 14 publications

(11 reference statements)

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“…However, the ability of various commercial kits to manually extract CMV DNA from spiked human specimens varies [ 34 ]. Further, compared to other clinical specimens, respiratory tract specimens have been reported to account for the highest CMV PCR detection rate [ 18 , 19 , 35 ], for which similar results were found in this study ( Table 2 and Table 3 ). Comparison of the sensitivities of different extraction protocols in various clinical specimens for CMV PCR requires further investigation.…”

Section: Discussionsupporting

confidence: 89%

Increasing Cytomegalovirus Detection Rate from Respiratory Tract Specimens by a New Laboratory-Developed Automated Molecular Diagnostic Test

et al. 2020

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Lots of automated molecular methods for detecting cytomegalovirus (CMV) DNA in the blood are available, but seldom for various clinical specimens. This study was designed to establish a highly sensitive automated assay to detect CMV DNA in non-blood specimens. We designed a new QMT assay using QIAGEN artus CMV RG polymerase chain reaction (Q-CMV PCR) kit applied on the BD MAX system and compared with the other assays, including an RGQ assay (LabTurbo auto-extraction combined Q-CMV PCR kit on Rotor-Gene-Q instrument), and in-house PCR assay. A total of 1067 various clinical samples, including 426 plasma, 293 respiratory tract specimens (RTS), 127 stool, 101 cerebral spinal fluid, 90 vitreous humours were analysed. Examining CMV DNA in simultaneous specimens of the same immunocompromised patient with respiratory symptoms, the detection rate of RTS (93.6%, 88/94) was significant higher than plasma (65.9%, 62/94). The positive rates for plasma samples with a low CMV viral load (<137 IU/mL) and diagnostic sensitivity of QMT, RGQ, and in-house assays were 65% and 99.1%, 45% and 100%, 5% and 65.5%, respectively. The QMT assay performs better, with shorter operational and turnaround time than the other assays, enabling the effective and early detection of CMV infection in various clinical specimens, particularly for RTS.

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Section: Discussionsupporting

confidence: 89%

Increasing Cytomegalovirus Detection Rate from Respiratory Tract Specimens by a New Laboratory-Developed Automated Molecular Diagnostic Test

et al. 2020

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“…It should be taken into account that not all the methods to detect CMV show the same efficacy, also depending, i.e ., on the available PCR kits. Moreover, differences can be also related to the analyzed samples [23, 128].…”

Section: Resultsmentioning

confidence: 99%

Human Breast Milk-acquired Cytomegalovirus Infection: Certainties, Doubts and Perspectives

Bardanzellu

Fanos

Reali

2019

CPR

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Breast Milk (BM) is the best source of nutrition for newborns, especially if premature. In fact, its beneficial impact on short- and the long-term neonatal outcome has was deeply described.Unfortunately, BM could not be always so safe, especially due to the possible presence of maternal viruses that can be shed and transferred to the breastfed neonate. Among these, Cytomegalovirus (CMV) can potentially lead to a serious and acute illness, mostly in case of low gestational age.Some studies also report the association of CMV-acquired infection to an increased risk of structural and functional brain modifications and neurological impairment.Due to these reasons, a strategy to remove CMV from BM with a minimal or absent impact on its beneficial components would be desirable.Up to now, pasteurization, freezing, ultraviolet- C or microwave irradiation are the available techniques; they show different levels of efficacy and variable effects on BM composition, even if many studies are still needed to fully clarify these implications.In this review, we provide an update of the current evidence about these topics. We focus on the factors promoting CMV shedding through BM; moreover, the possible occurrence of a severe disease in preterm neonates is also described. Finally, we investigate the potential effects showed on BM properties by the strategies that prevent or reduce viral transmission, therefore influencing newborns’ health, and the new techniques which could show a relevant role in the next future, such as metabolomics.

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“…Four CSF samples were positive for a bacterium (S. agalactiae [n ϭ 2], S. pneumoniae [n ϭ 2]) during the study period and were also included. Routine viral testing for EV, CMV, HHV-6, and VZV was performed using a combination of targeted, laboratorydeveloped tests (LDTs) that were validated according to Clinical Laboratory Improvement Amendments (CLIA) requirements (11)(12)(13)(14)(15). Routine testing of CSF for HSV-1/2 was performed using an LDT on samples received up until September 2016 and using an FDA-cleared, commercial method (Simplexa HSV 1&2 Direct; DiaSorin, Cypress, CA) thereafter (11).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis

et al. 2018

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Rapid and accurate laboratory tests are important for the timely diagnosis and treatment of central nervous system infections. The FilmArray meningitis/encephalitis (ME) panel (BioFire Diagnostics, Salt Lake City, UT) is an FDA-cleared, multiplex molecular panel that allows the detection of 14 pathogens (bacterial [ = 6], viral [ = 7], and fungal [ = 1] pathogens) from cerebrospinal fluid (CSF). In this study, we evaluated the performance characteristics of the FilmArray ME panel using clinical, residual CSF samples ( = 291) that tested positive by a routine method(s) (e.g., bacterial culture, individual real-time PCR assay) for a pathogen represented on the ME panel. Of note, a subset ( = 76) of the CSF specimens was collected during the prevaccine era and had been characterized as positive for a bacterial pathogen. The FilmArray ME panel demonstrated an overall percent positive agreement (PPA) of 97.5% (78/80) for bacterial pathogens, 90.1% (145/161) for viruses, and 52% (26/50) for Despite the low overall agreement (52%) between the ME panel and antigen testing for detection of, the percent positive agreement of the FilmArray assay for was 92.3% (12/13) when the results were compared directly to the results of routine fungal smear or culture. The FilmArray ME panel offers a rapid (∼60-min), syndrome-based approach for the detection of select meningitis and encephalitis pathogens.

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Comparison of Six Real-Time PCR Assays for Qualitative Detection of Cytomegalovirus in Clinical Specimens

Cited by 25 publications

References 14 publications

Increasing Cytomegalovirus Detection Rate from Respiratory Tract Specimens by a New Laboratory-Developed Automated Molecular Diagnostic Test

Increasing Cytomegalovirus Detection Rate from Respiratory Tract Specimens by a New Laboratory-Developed Automated Molecular Diagnostic Test

Human Breast Milk-acquired Cytomegalovirus Infection: Certainties, Doubts and Perspectives

Evaluation of a Commercial Multiplex Molecular Panel for Diagnosis of Infectious Meningitis and Encephalitis

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