Comparison of six methods of DNA extraction for the diagnosis of bovine brucellosis by real-time PCR

Preis, Ingred Sales; Moura, A.R.; Souza, Patrícia Gomes de; Filho, Paulo Martins Soares; Júnior, Antônio Augusto Fonseca

doi:10.1007/s00203-019-01675-3

Cited by 3 publications

(1 citation statement)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bovine brucellosis is caused by gram‐negative, non‐spore‐forming, non‐motile and non‐capsulated coccobacilli bacteria. Brucellosis is an important zoonotic disease (Corbel, 2006 ; von Bargen et al., 2012 ) and is responsible for public health and economic impacts in endemic areas (Preis et al., 2019 ; Scacchia et al., 2013 ). In cattle, the disease is generally brought about by ingesting contaminated feed or water with secretion, or aborted foetal remains from infected cows or by licking the vaginal discharges and genitals of aborted foetuses (Díaz, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Sero‐prevalence and risk factors associated with brucellosis in dairy cattle of Sylhet District, Bangladesh: A cross‐sectional study

Nath

Ahmed

Malakar

et al. 2023

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Background:Brucellosis is an emerging disease that causes a significant impact on productive and reproductive performance in dairy cattle. Though Brucella is a pivotal microorganism for dairy cattle, the scenario of brucellosis in Sylhet District is unknown. Objectives:A cross-sectional study was carried out to assess the prevalence and determinants associated with brucellosis in dairy cattle of Sylhet District.Methods: A total of 386 sera and data on determinants from 63 dairy herds were collected from 12 sub-districts using simple random sampling. The sera were tested with Rose Bengal Brucella antigen test, Brucella abortus plate agglutination test and serum agglutination test to find out the sero-positivity.Results: Overall, 17.09% (95% CI: 13.67-21.18) prevalence in cows were calculated.Relatively higher prevalence (56.08%; 95% CI: 42.23-70.32) was recorded in cows having parity ≥4 and were at higher risk (OR = 7.28) than the other cows with parity 0-3.

show abstract