Comparison of six inoculation techniques withColletotrichum acutatum on cold stored strawberry plants and screening for resistance to this fungus in French strawberry collections

Denoyes-Rothan, Beatrice; Guérin, G.

doi:10.1007/bf01877242

Cited by 11 publications

(5 citation statements)

References 13 publications

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“…Strawberry cultivars were either purchased from commercial strawberry nurseries or obtained as in vitro plantlets from the USDA-ARS Germplasm Repository, Corvallis, OR. Thirty-one cultivars [including cultivars with known anthracnose reactions: Elsanta, susceptible to C. acutatum (Denoyes and Baudry 1995;Denoyes-Rothan and Guérin 1996), Chandler, susceptible to C. fragariae and C. gloeosporioides (Chang and Smith 2007), and Pelican, resistant to C. fragariae and C. gloeosporioides (Osorio et al 2014;Smith et al 1998)] and 50 MSUS clones (including US70, US159, and US292, resistant to C. fragariae and C. gloeosporioides) were established in 10-cm plastic pots in a 1:1 mixture of Jiffy-Mix (Jiffy Products of America Inc., West Chicago, IL) and sand. Plants used in the study were propagated by rooting young runner plants in the Jiffy-Mix:sand potting mixture and maintained in a greenhouse at 28°C ± 10°C with a 16-h photoperiod, and fertilized every 16 weeks with 0.6 grams/pot of Osmocote (ICL Specialty Fertilizers -North America, Dublin, OH)14-14-14 time-released fertilizer.…”

Section: Methodsmentioning

confidence: 99%

Comparison of Whole Plant and Detached Leaf Screening Techniques for Identifying Anthracnose Resistance in Strawberry Plants

et al. 2018

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Anthracnose is a destructive disease of strawberry caused by several Colletotrichum species including C. acutatum, C. fragariae, and C. gloeosporioides. Identification of anthracnose resistant strawberry germplasm has commonly relied on inoculation of whole plants with isolates of these pathogens. In this study, whole plants and detached leaves from 81 germplasm lines were inoculated with a conidial suspension of isolates of C. acutatum, C. fragariae, and C. gloeosporioides, incubated in the dark at 30°C, 100% relative humidity, for 48 h, and assessed for disease severity based on symptoms on inoculated petioles and leaves. The correlation between the disease severity ratings of the whole plants rated 30 days after inoculation and detached leaves rated 5 days after inoculation was determined. Based on leaf symptoms and petiole lesions, the association between the whole plant leaf disease severity rating (DSR) and detached leaf DSR was positive (r = 0.70), and the association between the whole plant DSR and the detached leaf DSR was also positive (r = 0.66). Whole plant and detached leaf DSRs were used to assign each germplasm line to a resistance category, and a posthoc Tukey's test showed that the whole plant DSR means and the detached leaf DSR means for each resistance category differed significantly at p < 0.05. This research was used to develop a strawberry detached leaf assay which can reliably and quickly determine the degree of resistance of strawberry germplasm to anthracnose.

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Section: Methodsmentioning

confidence: 99%

Comparison of Whole Plant and Detached Leaf Screening Techniques for Identifying Anthracnose Resistance in Strawberry Plants

et al. 2018

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“…Plants in inoculation experiments were grown in pots (0.5-liter volume) in peat-vermiculite medium (vol/vol; 1:1), watered twice daily by overhead or drip irrigation (depending on the experimental design), and maintained in a greenhouse at 25°C. The plants were artificially inoculated by spraying with a mixture of the C. acutatum isolates (TUT-79, -110, -137, -149, and -5954) at a concentration of 5 × 10 6 conidia/ml until run off, and maintained under 100% relative humidity by covering with plastic bags for 72 h, as similarly described (7,8,20). Leaves were sampled for assessment of survival and colonization at different time points starting immediately after spraying and until termination of the experiment.…”

Section: Fungal Cultures and Growth Conditions The Monoconidialmentioning

confidence: 99%

Pathogenic and Nonpathogenic Lifestyles in Colletotrichum acutatum from Strawberry and Other Plants

Freeman¹,

Horowitz²,

Sharon³

2001

Phytopathology®

140

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Anthracnose is one of the major fungal diseases of strawberry occurring worldwide. In Israel, the disease is caused primarily by the species Colletotrichum acutatum. The pathogen causes black spot on fruit, root necrosis, and crown rot resulting in mortality of transplants in the field. The host range and specificity of C. acutatum from strawberry was examined on pepper, eggplant, tomato, bean, and strawberry under greenhouse conditions. The fungus was recovered from all plant species over a 3-month period but caused disease symptoms only on strawberry. Epiphytic and endophytic (colonization) fungal growth in the different plant species was confirmed by reisolation from leaf tissues and by polymerase chain reaction (PCR)-specific primer amplification. C. acutatum was also isolated from healthy looking, asymptomatic plants of the weed genera Vicia and Conyza. Isolates that were recovered from the weeds caused disease symptoms on strawberry and were positively identified as C. acutatum by PCR. The habitation of a large number of plant species, including weeds, by C. acutatum suggests that, although it causes disease only on strawberry and anemone in Israel, this fungus can persist on many other plant species. Therefore, plants that are not considered hosts of C. acutatum may serve as a potential inoculum source for strawberry infection and permit survival of the pathogen between seasons.

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“…Selection for additional cultivated strawberry genotypes resistant to anthracnose crown rot is a credible option for managing this disease (Ballington et al, 2002;Denoyes-Rothan and Guérin, 1996;Gupton and Smith, 1991;Hancock et al, 1991Hancock et al, , 1996Simpson et al, 1994;Smith et al, 1996). However, apparent ''field resistance'' (Olcott-Reid and Moore, 1995) may be due to genotype by environment interactions unfavorable to infection or spread, rather than to true genetic disease resistance (Smith and Black, 1987).…”

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confidence: 99%

Evaluation of Elite Native Strawberry Germplasm for Resistance to Anthracnose Crown Rot Disease Caused by Colletotrichum Species

Lewers¹,

Turechek²,

Hokanson³

et al. 2007

J. Amer. Soc. Hort. Sci.

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Anthracnose crown rot of cultivated strawberry (Fragaria ×ananassa Duchesne ex Rozier) has been a major disease problem in the strawberry producing regions of the southeastern United States since the early 1970s. Chemical controls are often inadequate, but use of resistant cultivars is seen as a credible option for managing this disease. Only a small portion of Fragaria L. germplasm has been screened for resistance to anthracnose crown rot. A core subset of the Fragaria collection maintained at the U.S. Department of Agriculture National Clonal Repository in Corvallis, OR, has been constructed to contain an elite group of native F. virginiana Mill. and F. chiloensis (L.) Mill. This collection, referred to as the “core collection,” has been characterized for many horticultural traits, including reactions to several common foliar diseases, resistance to black root rot (causal organisms unknown), and resistance to northern root-knot nematode (Meloidogyne hapla Chitwood) and root-lesion nematode [Pratylenchus penetrans (Cobb) Filipjev & Shuurmans Stekhoven]. Our objective was to evaluate the core collection for resistance to a selection of isolates of three Colletotrichum Corda species known to cause strawberry anthracnose, Colletotrichum fragariae A.N. Brooks, Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in Penz. [teleomorph Glomerella cingulata (Stoneman) Spauld. & H. Schrenk], and Colletotrichum acutatum J.H. Simmonds (teleomorph Glomerella acutata J.C. Guerber & J.C. Correll). No Fragaria subspecies or geomorph was more resistant than any other; rather, individual genotypes within these groups were identified as sources from which resistance can be obtained. Collecting germplasm in areas of intense disease pressure may not be as beneficial as one might assume, at least where anthracnose crown rot disease is concerned.

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Comparison of six inoculation techniques withColletotrichum acutatum on cold stored strawberry plants and screening for resistance to this fungus in French strawberry collections

Cited by 11 publications

References 13 publications

Comparison of Whole Plant and Detached Leaf Screening Techniques for Identifying Anthracnose Resistance in Strawberry Plants

Comparison of Whole Plant and Detached Leaf Screening Techniques for Identifying Anthracnose Resistance in Strawberry Plants

Pathogenic and Nonpathogenic Lifestyles in Colletotrichum acutatum from Strawberry and Other Plants

Evaluation of Elite Native Strawberry Germplasm for Resistance to Anthracnose Crown Rot Disease Caused by Colletotrichum Species

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