Comparison of single nucleotide polymorphisms in the human interleukin-10 gene promoter between rheumatoid arthritis patients and normal subjects in Malaysia

Hee, Chee Seng; Gun, Suk Chyn; Naidu, Rakesh; Gupta, Ekta; Somnath, Sushela Devi; Radhakrishnan, Ammu Kutty

doi:10.3109/s10165-007-0645-0

Cited by 2 publications

(4 citation statements)

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“…However, the frequencies of the IL‐10 SNPs differ significantly from the Caucasian cohorts 10,22 . As reported previously, the SNPs at positions –824 and –597 in the promoter of the human IL‐10 gene were found to be in complete linkage disequilibrium and the genotype distribution was found to be identical for these two polymorphisms 19 …”

Section: Discussionsupporting

confidence: 52%

“…However, we were unable to detect any significant correlations between the SNP haplotypes and SLE incidence when the data was analyzed individually. Other investigators have found no association with Chinese, 19 Dutch 29 and British 30 SLE patients. The GCC haplotype was correlated with anti‐Ro (SSA) autoantibodies in British Caucasians 31 while the ATA haplotype was reported to be associated with renal disease in Chinese SLE patients 26 but not in Dutch 29 or British SLE patients 30 .…”

Section: Discussionmentioning

confidence: 88%

“…The production of IL‐10 by SLE patients and their family members was reported to be significantly higher than the healthy and unrelated control subjects 4,5 . The association of SNP in the promoter region of the human IL‐10 gene and disease susceptibility has been studied in several diseases such as myocardial infarction and coronary artery disease, 16 Alzheimer's disease, 17 SLE, 18 rheumatoid arthritis 19 and schizophrenia 20 . Some of the reported allele frequencies for the three SNPs in the promoter of the human IL‐10 gene reported for different populations and diseases are shown in Table 1.…”

Section: Introductionmentioning

confidence: 99%

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The relationship between single nucleotide polymorphisms of the interleukin‐10 gene promoter in systemic lupus erythematosus patients in Malaysia: a pilot study

Hee¹,

Gun²,

Naidu³

et al. 2008

Int J of Rheum Dis

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Aim: Recent studies have shown that single nucleotide polymorphisms (SNPs) have been identified within the promoter of the human interleukin‐10 (IL‐10) gene may participate in the pathogenesis of systemic lupus erythematosus (SLE) and may be related to disease activity. This is a pilot study that investigated the allelic and genotype frequencies of three SNPs in the human IL‐10 gene promoter [rs1800896 (position: –1082G > A), rs1800871 (position: –824C > T) and rs1800872 (position: –597C > A)] among Malaysian SLE patients and normal subjects. Methods: Blood was drawn from 44 SLE patients and 44 age‐ and sex‐matched healthy control subjects for DNA extraction. The SNPs were identified using the polymerase chain reaction‐restriction fragment length polymorphism (PCR‐RFLP) method. Results: There was no significant difference in the genotype and allele frequencies between the SLE patients and control subjects. A statistically significant difference was detected in the haplotype frequencies between the patients and controls (P = 0.004). Conclusions: There is a significant difference in the haplotype frequencies between the SLE patients and controls; the SNPs in the human IL‐10 gene promoter could play an important role in the pathogenesis of SLE.

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Section: Discussionsupporting

confidence: 52%

Section: Discussionmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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The relationship between single nucleotide polymorphisms of the interleukin‐10 gene promoter in systemic lupus erythematosus patients in Malaysia: a pilot study

Hee¹,

Gun²,

Naidu³

et al. 2008

Int J of Rheum Dis

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“…Genomic DNA was extracted from peripheral blood leukocytes using a genomic isolation DNA commercial kit as recommended by the manufacturer (QIAGEN, Germany). The extracted DNA was amplified by PCR using published forward and reverse primers (Padyukov et al 2004; Hee et al 2007) (LNTRON Biotechnology, Korea). PCR was performed using commercially available PCR premix according to the manufacturer's recommendation protocol (DMTRON Biotechnology, Korea) and a PTC‐100 Pltier thermal cycle (MJ Research).…”

Section: Methodsmentioning

confidence: 99%

Is there a genetic variation association in the IL-10 and TNF α promoter gene with gestational diabetes mellitus?

2010

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Gestational diabetes mellitus (GDM), defined as carbohydrate intolerance diagnosed for the first time during pregnancy, affects both maternal and fetal health. Possession of a specific genetic polymorphism can be a predisposing factor for susceptibility to some diseases. The aim of this study was to investigate the association between single nucleotide polymorphisms (SNP) in the promoter gene of interleukin-10 (IL-10) as well as tumor necrosis factor-alpha (TNF alpha) with the development of GDM. Two hundred and twelve consecutive series of eligible normal pregnant women (controls) and gestational diabetes mellitus women were selected based on the study's inclusion and exclusion criteria. DNA was extracted from blood and genotyped for IL-10 at three positions and TNF alpha for gene polymorphism using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Plasma levels of IL-10 and TNF alpha at different gestational periods as well as postpartum were quantified using enzyme linked immunosorbent assay (ELISA). The results of the study showed that the difference in the frequency of SNP at position -597 in the promoter of the human IL-10 gene between the control and GDM groups was statistically significant (p < 0.05). In contrast, there was no significant difference in the frequency of SNP at the other two sites in the promoter region of the human IL-10 gene (-824 and -1082) as well as position -308 in the promoter of the human TNF-alpha (p > 0.05). In addition, there was no significant difference between the two groups in terms of plasma levels of IL-10 as well as TNF alpha in different stages of pregnancy. SNP at position -597 was significantly associated with the development of GDM and shows potential for use as a predictive marker for GDM.

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Comparison of single nucleotide polymorphisms in the human interleukin-10 gene promoter between rheumatoid arthritis patients and normal subjects in Malaysia

Cited by 2 publications

References 0 publications

The relationship between single nucleotide polymorphisms of the interleukin‐10 gene promoter in systemic lupus erythematosus patients in Malaysia: a pilot study

The relationship between single nucleotide polymorphisms of the interleukin‐10 gene promoter in systemic lupus erythematosus patients in Malaysia: a pilot study

Is there a genetic variation association in the IL-10 and TNF α promoter gene with gestational diabetes mellitus?

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