Comparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties

Guerra, Patrícia Araújo; Souza, Everton Hilo de; Andrade, E. C. de; Max, Daniela de Andrade Silva; Oliveira, Rafaelle Souza de; Souza, Fernanda Vidigal Duarte

doi:10.1007/s11627-020-10100-0

Cited by 9 publications

(3 citation statements)

References 27 publications

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“…All cryoderived plants were free of ACLSV and ASPV, and 80% of the cryoderived plants were free of ASGV confirmed by reverse transcription (RT)‐PCR in plants after 8 months of growth in the greenhouse. Applying droplet‐vitrification cryotherapy to the shoot tips of three pineapple accessions mix‐infected with pineapple mealybug wilt‐associated virus (PMWaV), Guerra et al (2020) found that all cryoderived plants of the accessions AGB‐009 and AGB‐376 were virus‐free, while only 50% of the accession AGB‐119 were virus‐free.…”

Section: Shoot Tip Cryotherapy For Pathogen Eradicationmentioning

confidence: 99%

Shoot tip cryotherapy for plant pathogen eradication

Bettoni

Zhang

et al. 2022

Plant Pathology

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Diseases caused by plant pathogens such as viruses, viroids and phytoplasmas cause huge economic losses of agricultural production and limit the safe movement of plant materials across borders. The use of pathogen-free planting materials provides a strategy for efficient management of these diseases and facilitates the global exchange of genetic resources. Shoot tip cryotherapy is a novel biotechnology method that uses cryogenic procedures to eradicate plant pathogens from the diseased plants.Combining thermotherapy or chemotherapy with shoot tip cryotherapy has further enhanced pathogen eradication efficiency. This review provides updated and comprehensive information on shoot tip cryotherapy and the combination of thermotherapy or chemotherapy with shoot tip cryotherapy for pathogen eradication. Prospects are proposed for future studies.

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Section: Shoot Tip Cryotherapy For Pathogen Eradicationmentioning

confidence: 99%

Shoot tip cryotherapy for plant pathogen eradication

Bettoni

Zhang

et al. 2022

Plant Pathology

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“…In fact, large tissues can also be cultured, if only to obtain in vitro plants, and it is much easier to obtain a big tip than a small one. Studies have found that smaller shoot tip culture can remove viruses from infected plants [ 15 , 16 , 17 ], but the effect of longer shoot tip culture on viruses is unclear. Grapevine is a fruit crop with major relevance worldwide and is also among the most well-studied deciduous woody perennials.…”

Section: Introductionmentioning

confidence: 99%

Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses

Dong

Zhang

et al. 2022

Plants

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Shoot tip culture is a very effective approach for studying plant viruses. In this study, we evaluated the numbers, diversity, and titer of grapevine viruses in in vitro grapevine plants after long shoot tip culture. Six virus-infected grapevine cultivars (Cabernet Franc, Cabernet Gernischt, Cabernet Sauvignon, Wink, Victoria, and Merlot) collected from six regions of China were used as the research materials. Approximately 1.5 cm long shoot tips were used for meristem culture. The average survival rate of the six grapevine cultivars was 45.7%. Merlot collected from Beijing showed the highest survival rate (80.0%). Regeneration was not achieved in Cabernet Gernischt collected from Liaoning province and Cabernet Sauvignon from Tianjin due to bacterial and fungal contamination. Virus detection conducted in the surviving regenerated plants showed that the virus infection status, including the viral numbers and the species present in plants grown in vitro, was the same as that in corresponding in vivo plants. Moreover, the analysis of sequence diversity and the mutation frequency in grapevine viruses in vitro indicated that the structure of grapevine viruses was stable in long shoot tip culture after four sub-culture passages. Further, the relative viral titer of in vitro grapevine plants was much higher than that of in vivo plants. These results aid in the investigation of viruses in woody plants.

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“…A diferença mais expressiva da classificação taxonômica atual em relação à anterior é referente às cinco variedades botânicas serem anteriormente consideradas espécies e a espécie A. macrodontes que pertencia ao gênero Pseudananas, sendo conhecida como P. sagenarius (Smith & Downs, 1979 ), desta forma ignorando atributos exigidos pela tual classificação, entre elas a ausência de barreiras de incompatibilidade de reprodução entre indivíduos de espécies diferentes (Leal, D'Eeckenbrugge, & Holst, 1998). A maior coleção de germoplasma de abacaxi encontra-se na Embrapa Mandioca e Fruticultura, uma unidade da Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), em Cruz das Almas, Bahia, constituído atualmente por aproximadamente 700 acessos (Guerra et al, 2020& Souza et al 2012. O Banco Ativo de Germoplasma de abacaxi (BAG-abacaxi) é preservado ex situ, em condições de campo, onde há uma duplicata de segurança que consiste em uma planta por vaso numa casa de vegetação, e ainda possui uma cópia de segurança in vitro desde 2003 (da Silva, 2016& Souza et al 2012.…”

Section: Introductionunclassified

Estudos metabolômicos de >i<Ananas comosus >/i<e >i<A. macrodontes >/i<(Bromeliaceae)

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Comparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties

Cited by 9 publications

References 27 publications

Shoot tip cryotherapy for plant pathogen eradication

Shoot tip cryotherapy for plant pathogen eradication

Effect of In Vitro Culture of Long Shoot Tip on Variant Structure and Titer of Grapevine Viruses

Estudos metabolômicos de >i<Ananas comosus >/i<e >i<A. macrodontes >/i<(Bromeliaceae)

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