Comparison of Seven Commercial Antigen and Antibody Enzyme-Linked Immunosorbent Assays for Detection of Acute Dengue Infection

Blacksell, Stuart D.; Jarman, Richard G.; Gibbons, Robert V.; Tanganuchitcharnchai, Ampai; Mammen, Mammen P.; Nisalak, Ananda; Kalayanarooj, Siripen; Bailey, M S; Premaratna, R.; Silva, H. Janaka de; Day, Nicholas P. J.; Lalloo, David G.

doi:10.1128/cvi.05717-11

Cited by 115 publications

(120 citation statements)

References 24 publications

(31 reference statements)

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“…However, an early and precise dengue diagnostic is important for patient management, to avoid dengue severity and mortality and, ultimately, to control epidemics. 8,21,26 Our results agree with others who reported that the combination of two or more diagnostic strategies is needed to improve the diagnostic accuracy of DENV infection. 8,[21][22][23]26 Combining direct DENV detection and antibody-based tests seems to be a particularly promising strategy.…”

Section: Discussionsupporting

confidence: 91%

“…8,21,26 Our results agree with others who reported that the combination of two or more diagnostic strategies is needed to improve the diagnostic accuracy of DENV infection. 8,[21][22][23]26 Combining direct DENV detection and antibody-based tests seems to be a particularly promising strategy. However, as seen in this study and evidenced by the low LR+ and LR− values, combinations of current assays do not guarantee the accuracy of the diagnosis when compared with standards such as PRNT or ELISPOT-MNT.…”

Section: Discussionsupporting

confidence: 91%

“…However, it has to be stressed that many reports that granted acceptable performances to commercially available dengue assays used ELISAs as comparators instead of gold standard methods such as PRNT or HI. 8,21,23,26 A recent study showed that ELISPOT-MNT had a sensitivity of 95.6% and specificity of 88.2%, with a good correlation (R 2 = 0.7) with PRNT. 16 Because of its very high sensitivity, the use of ELISPOT-MNT as the comparative standard method in our study leads inevitably to lower statistics performances of the assays that are compared with it, which can explain the relatively low performances of both diagnostic strategies.…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Commercially Available Assays for Diagnosis of Acute Dengue in Schoolchildren During an Epidemic Period in Medellin, Colombia

Piedrahita

Agudelo

Trujillo

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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Abstract. During an active surveillance study in school children in Medellin, we assessed the performance of two diagnostic strategies for dengue virus. A total of 41 patients with suspected dengue acute infection were evaluated. Diagnostic strategies consisted of one combining Panbio ® Dengue virus IgM and IgG Capture ELISAs (enzyme-linked immunosorbent assays) with reverse transcriptase polymerase chain reaction (RT-PCR) and another using a commercial rapid SD Bioline Dengue Duo (IgG/IgM + NS1 Ag) test. These two strategies were compared with the enzymelinked immunospot microneutralization test (ELISPOT-MNT). The sensitivity and specificity were 53.9% and 80.0% for the combination of Panbio ® ELISAs and RT-PCR tests, and 30.8% and 73.3% for the SD Bioline Duo test, respectively. ELISPOT-MNT detected 16.4% additional cases and revealed the presence of neutralizing antibodies in all the acute samples, evidencing that they were all secondary infections. In contrast, Panbio ® and SD Dengue Duo rapid tests only classified 23.0% and 26.9% of the cases as secondary dengue infections, respectively. Cohen's kappa coefficient and McNemar's association tests demonstrated a significant disagreement between the two diagnostic strategies and ELISPOT-MNT. Overall, these results evidence the relatively poor performances of commercial assays for the diagnosis of acute and secondary dengue infections, compared with ELISPOT-MNT, and raise concern about the accuracy of these assays for the diagnostic of dengue in endemic areas.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 99%

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Evaluation of Commercially Available Assays for Diagnosis of Acute Dengue in Schoolchildren During an Epidemic Period in Medellin, Colombia

Piedrahita

Agudelo

Trujillo

et al. 2016

The American Journal of Tropical Medicine and Hygiene

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“…A cut-off value was determined and results were interpreted as positive and negative according to manufacturer's instructions [13,14] .…”

Section: Sample Processingmentioning

confidence: 99%

Dengue Virus Infection among Voluntary Blood Donors in Osogbo, Southwestern Nigeria

Muhibi¹,

Adeleke²,

Shittu³

et al. 2017

Am. J. Biomed. Sci.

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Dengue virus is one of the causative agents of viral haemorrhagic fever, which is transmitted primarily through Aedes mosquitoes. Transmission of the virus through non-vector sources had been proven, although, it is the most common arthropod-borne viral infection in human. This study was carried out to determine the prevalence of Dengue Virus amongst voluntary blood donors in Osogbo, southwest Nigeria. Ninety one voluntary non-remunerated donors that satisfied donor suitability criteria in Nigeria; having been considered fit based on physical assessment, responses provided to Blood Donors' questionnaire, and tested negative by both rapid diagnostic and ELISA techniques to HIV 1 & 2, HBV, HCV and syphilis were screened for antiDengue IgM antibody using Enzyme Linked Immuno-Sorbent Assay (ELISA). It was observed that most of the donors (56.04%) fall within age group 28-37 years, while none of the participant was older than 47. In terms of gender distribution, men (86.81%) were in the majority. Marital profile showed that most of the participants were unmarried (72.53%); while literates account for 58.24%. The seroprevalence of IgM antibodies showed that only two (2.2%) participants were positive for Dengue virus. Result showed that there is low prevalence of Dengue virus among voluntary blood donors in Osogbo. Monitoring the trends in prevalence of transmissible infectious agents in blood donors will provide a mechanism to evaluate the safety of the blood supply. It is hereby recommended that surveillance be sustained on all emerging and reemerging infectious diseases that have potentials of being transmitted through blood transfusion, dengue virus inclusive.

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“…The IgA has been commented with an analogous behaviour to IgG antibody. In primary infection must be detectable after the release of IgM, while in secondary infection IgA appear early in acute phase by the common epitopes recognition by memory B cell clones circulating on blood [40] [42]. The induction of high-rate IgA levels requires a group of factors which must act synergistically in the Ig production predominantly toward IgA isotype: engagement of CD40 (B cells) by CD40 ligand (CD40L, Ag-activated CD4+ T cells) which determines class switch gene recombination; TGF-ß secreted by CD40L-induced B cells is required for switching to IgA; the presence of IL-10 increasing both the number of IgA lymphocytes and the amount of secreted IgA.…”

Section: Discussionmentioning

confidence: 99%

Virological and Serological Markers in Dengue Patients from Venezuela and Nicaragua

Ruiz¹,

Vázquez²,

Villegas³

et al. 2017

OALib

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Latest estimates suggest 390 million infections of dengue occur each year, of which 100 million results in symptomatic disease. The new clinical classification proposed by WHO-TRD 2009 simplifies dengue fever (DF) into dengue (D) and severe dengue (SD) with an intermediary group of "dengue with warning signs" (DWS). Early, sensitive and specific diagnosis of dengue is needed for appropriate patient management as well as for early epidemic detection. Virological and serological markers in sera from dengue confirmed patients from Venezuela and Nicaragua were evaluated. A total of 232 sera collected in the acute (days 1 to 5 of fever onset) and convalescent (days 7 to 14) phases of illness were tested by Platelia NS1 antigen ELISA and IgM, IgA and IgE capture ELISA methods. NS1 was detected with highest OD values in the acute phase for primary and secondary cases. IgM was detected in primary and secondary groups but the highest OD figures were observed after defervescence day. IgA and IgE detection were almost absent for primary cases, contrary to secondary cases which showed higher values but after defervescence day. NS1 could be used as effective diagnostic marker in the acute phase of illness for primary and secondary dengue infections. IgM detection is still an invaluable tool for routine dengue diagnosis but delay. IgA detection could define secondary infection in early phase of illness. IgA/IgE could be evaluated as prognostic markers associated to SD cases.

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Comparison of Seven Commercial Antigen and Antibody Enzyme-Linked Immunosorbent Assays for Detection of Acute Dengue Infection

Cited by 115 publications

References 24 publications

Evaluation of Commercially Available Assays for Diagnosis of Acute Dengue in Schoolchildren During an Epidemic Period in Medellin, Colombia

Evaluation of Commercially Available Assays for Diagnosis of Acute Dengue in Schoolchildren During an Epidemic Period in Medellin, Colombia

Dengue Virus Infection among Voluntary Blood Donors in Osogbo, Southwestern Nigeria

Virological and Serological Markers in Dengue Patients from Venezuela and Nicaragua

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