Comparison of Seven Cell Lines Derived From Human Gastric Carcinomas

Abstract

In an attempt to elucidate various histological features of gastric cancers, seven human gastric adenocarcinomas were studied in vitro and in nude mice. Growth pattern of each cultured cell line in vitro corresponded well to the histological type of parent tumor. The cell lines, MKN7, MKN74, and MKN28 derived from differentiated carcinomas showed morphological characteristics of intestinal differentiation in cell polarity and microvilli with core-filaments in vitro as well as in nude mice. However, they gradually diminished the characteristics in course of time. The cell lines, MKN 45 and OKAJIMA, derived from undifferentiated carcinomas, had natures of not only ordinary gastric mucosa but also intestinal metaplastic mucosa. They seem to have multipotentiality for differentiation, and preserved well the natures for long periods of culture. The KWS-I cell line composed of undifferentiated cells in vitro displayed the potential for differentiation in nude mice. However, the differentiation of KATO-I11 cells derived from a signet-ring cell carcinoma was suppressed in nude mice. The common abnormality of chromosome was not found, and the growth rate in vitro was not dependent on the histological type of parent tumor. ACTA PATHOL. JPN. 36: 65-83, 1986. I n t reductionBecause of the high incidence of gastric carcinoma in Japan and its various histological features, deep interest has been taken in its carcinogenesis, histogenesis, growth pattern, and cell differentiation. The cultivation of human cancer cells has been expected to be one of the methods for making these subjects clearer.Cell lines of human gastric carcinoma over fifteen at least have been established till now in Japan. However, little attention has been given to comparing these cell lines with each other and with each parent tumor.The present study was undertaken to compare seven cell lines derived from human gastric carcinomas with each other in respects of biology and morphology in vitro and in nude mice. long term culture were inspected. Furthermore, stable and unstable natures of parent tumor cells through Materials and MethodsCell lines ; Seven cultured cell lines derived from gastric adenocarcinomas including a signetring cell carcinoma were used in this study ( Table 1). The cell line MKN7 was derived from a well differentiated tubular adenocarcinoma. Two cell lines, MKN74 and MKN28, were derived from moderately differentiated tubular adenocarcinomas. The MKN45 cell line was derived from a poorly differentiated adenocarcinoma of medullary type. The KWS-I cell line, kindly donated by Dr. M. Sekiguchi ), was derived from a poorly differentiated adenocarcinoma with focal scirrhous pattern. The KATO-I11 cell line, kindly donated by Dr. M. Sekiguchi, was derived from a signetring cell carcinoma. The culture courses, drug-M phosphate buffer (pH 7.4) for 30 minutes. Tissue cubes were fixed in 2.5% glutaraldehyde 0.1 M phosphate buffer (pH 7.4) for 1 hour. Both of them were postfixed in 1% osmium tetraoxide for 1 hour, and embedded in Epon-812. Ultrathin s...

THBS4, a novel stromal molecule of diffuse-type gastric adenocarcinomas, identified by transcriptome-wide expression profiling

“…33 OCUM and fibroblast cell lines were routinely kept in high glucose DMEM (4.5 g/l) with 10% FBS, 100 U/ml penicillin, 100 mg/ml streptomycin, 0.5 mM sodium pyruvate and 2 mM L-glutamine at 37 1C in 5% CO 2 (everything PAA Laboratories, Pasching, Austria). MKN-45 cells 34 were cultured according to the protocol outlined by the provider (German Collection of Microorganisms and Cell Cultures).…”

“…31,32,34 In vitro, OCUM cells exclusively grow in suspension (single or in cell clusters), whereas MKN-45 cells predominantly grow adherent as mono or multilayers with only few cells being suspended. This behavior is not characteristic for diffuse-type gastric tumors or their cell lines.…”

Distinct methylation patterns of two APC gene promoters in normal and cancerous gastric epithelia

“…Ten gastric cancer cell lines with di ering histologies were cultured under appropriate conditions in our laboratory; MKN1, an adenosquamous cell carcinoma; MKN7, a well di erentiated adenocarcinoma; MKN28 and MKN74, moderately di erentiated adenocarcinomas, MKN45 and KWS-I; poorly di erentiated adenocarcinomas; KATO-III, a signet ring cell carcinoma; ECC10 and ECC12, endocrine cell carcinomas; and TSG11, a hepatoid carcinoma (Motoyama et al, 1986). Forty gastric cancers and their matching normal gastric mucosae were obtained surgically from 40 Japanese patients.…”

Production of endothelin-1 and thrombomodulin by human pancreatic cancer cells

“…MKN-28 was kindly provided by H. Watanabe (Niigata University School of Medicine, Niigata, Japan) (Motoyama et al, 1986). The other cell lines were purchased from the American Type Culture Collection (Rockville, MD, USA).…”