Comparison of serum NO, TNF-α, IL-1β, sIL-2R, IL-6 and IL-8 levels with grades of retinopathy in patients with diabetes mellitus

We measured interleukin-1 beta (IL-1b) and tumour necrosis factor-alpha (TNF-a) in the vitreous humour and serum of patients with proliferative diabetic retinopathy (PDR), in order to determine the role of these cytokines in the pathogenesis of the disease. Vitreous and serum samples were collected from 21 patients with PDR who were undergoing pars plana vitrectomy. Control vitreous samples were obtained from cadavers and control serum samples from healthy subjects. The cytokines were measured by enzymelinked immunosorbent assay. Vitreous IL-1b and TNF-a concentrations in patients with PDR exceeded those of controls (Po0.05), as did serum IL-1b and TNF-a. We suggest that increased vitreous IL-1b and TNF-a levels may play a significant role in the pathogenesis of PDR, which features abnormal cell proliferation and neovascularisation.

show abstract

“…[17][18][19][20] The biological effects of IL-1b depend on the target cell. Together with TNF-a, IL-1b is responsible for angiogenic activity.…”

Section: Tnf-α_vmentioning

confidence: 99%

Determination of vitreous interleukin-1 (IL-1) and tumour necrosis factor (TNF) levels in proliferative diabetic retinopathy

Demircan

Safran

Soylu

et al. 2005

Eye

337

221

View full text Add to dashboard Cite

show abstract

“…The concentration of NO 2 -in serum was measured according to the method of Griess, described by Doganay et al [38]. 100 μl of serum was added in duplicate to an Eppendorf The frequency of coughing was assessed using a 10 cm coughing visual analogue scale with the extremes marked 1 -'I never cough' and 10 -'I am coughing all the time'.…”

Section: Determination Of Hydrogen Peroxide (H 2 O 2 ) In Ebcmentioning

confidence: 99%

Apocynin decreases hydrogen peroxide and nirtate concentrations in exhaled breath in healthy subjects

Stefańska

Sokołowska

Sarniak

et al. 2010

Pulmonary Pharmacology & Therapeutics

View full text Add to dashboard Cite

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. M A N U S C R I P T A C C E P T E D ARTICLE IN PRESS AbstractThe imbalance between reactive oxygen species (ROS) synthesis and antioxidants might be involved in the pathogenesis of many inflammatory diseases. NADPH oxidase, an enzyme responsible for ROS production, may represent an attractive therapeutic target to inhibit for the treatment of these diseases.Apocynin is an inhibitor of activation of NADPH oxidase complex present in the inflammatory cells.In double blind, placebo controlled, cross-over study, we investigated the effect of nebulized apocynin on ROS synthesis in 10 nonsmoking healthy volunteers. Apocynin (6 ml of 0.5 mg/ml) was administered by nebulization and its effects on H 2 O 2 , NO 2 -and NO 3 -generation were assessed after 30, 60 and 120 minutes by collecting exhaled breath condensate (EBC) samples using an EcoScreen analyzer. Additionally, respiratory parameters have been evaluated, utilizing spirometry and DLCO. We also analyzed peripheral blood differential counts and NO 2 -serum level, cough scale control and blood pressure as safety parameters. Thus, as apocynin significantly influence ROS concentration, it might have also antiinflammatory properties. As it is safe, it may have a potential to become a drug in airway inflammatory diseases treatment.

show abstract

“…Recently, great progress has been achieved in identifying soluble factors in the circulation and vitreous fluid, such as TNF-a, VEGF, and sICAM-1, which are associated with the development and progression of DR. [2][3][4][5][6][7][8] A recent study demonstrated that the canonical Wnt signaling pathway is activated in the retina in humans with DR and in DR animal models, and it has a causative role in DR. 9 The Wnt/b-catenin signaling pathway is an important signaling pathway that regulates multiple biological and pathological processes including cell proliferation and differentiation, apoptosis, stem cell maintenance, angiogenesis, inflammation, fibrosis, and carcinogenesis. 10 This signaling pathway is regulated by a number of natural inhibitors including Dickkopf-1 (DKK-1), a well-established specific inhibitor of the Wnt signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

Plasma and vitreous fluid levels of Dickkopf-1 in patients with diabetic retinopathy

Qiu

Yan

et al. 2014

Eye

View full text Add to dashboard Cite

Purpose Dickkopf-1 (DKK-1) is a secreted inhibitor of the Wnt/b-catenin signaling pathway, which plays a pathogenic role in diabetic retinopathy (DR). We aimed to investigate whether DKK-1 levels in the plasma and the vitreous are associated with DR in type 2 diabetes mellitus (DM) patients. Methods Case-control study: plasma samples were collected from 125 type 2 DM including 81 DR (29 non-proliferative DR (NPDR) and 52 proliferative DR (PDR)), 44 non-DR patients (NDR), and 100 non-diabetic controls. Undiluted vitreous fluid samples were obtained from 30 PDR and 25 nondiabetic patients. DKK-1 concentrations in samples were determined using enzymelinked immunosorbent assay. Variables were compared with the Kruskal-Wallis H test, Mann-Whitney U-test, and w 2 -test, when appropriate. Results Plasma DKK-1 levels were significantly lower in DR patients (median: 465.77 pg/ml, range: 137.11-1190.31) than in non-diabetic controls (656.83 pg/ml, 171.63-1795.08; Po0.001) and NDR patients (693.04 pg/ml, 305.43-1218.35; Po0.001). Furthermore, DKK-1 levels were lower in PDR patients (425.21 pg/ml, 137.10-1077.32) compared with NPDR patients (594.86 pg/ml, 256.36-1393.27; P ¼ 0.003). Vitreous absolute DKK-1 levels in PDR patients (259.04 pg/ml, 104.44-596.96) were higher than in nondiabetic controls (138.26 pg/ml, 18.69-239.52; Po0.001). After normalizing by total vitreous protein concentrations, however, there was no significant difference between the groups. DKK-1 levels in vitreous were lower than those in plasma in both groups (Po0.001 for controls; P ¼ 0.002 for PDR patients).Conclusions Decreased plasma DKK-1 levels, which may contribute to the Wnt pathway activation, are associated with the presence and progression of DR, and have potential to become a biomarker for DR.

show abstract

Comparison of serum NO, TNF-α, IL-1β, sIL-2R, IL-6 and IL-8 levels with grades of retinopathy in patients with diabetes mellitus

Cited by 152 publications

References 28 publications

Determination of vitreous interleukin-1 (IL-1) and tumour necrosis factor (TNF) levels in proliferative diabetic retinopathy

Determination of vitreous interleukin-1 (IL-1) and tumour necrosis factor (TNF) levels in proliferative diabetic retinopathy

Apocynin decreases hydrogen peroxide and nirtate concentrations in exhaled breath in healthy subjects

Plasma and vitreous fluid levels of Dickkopf-1 in patients with diabetic retinopathy

Contact Info

Product

Resources

About