Comparison of Serum Humoral Responses Induced by Oral Immunization with the Hepatitis B Virus Core Antigen and the Cholera Toxin B Subunit

Broos, Kathleen; Janssens, Michiel Etienne; Goeyse, Ine De; Vanlandschoot, Peter; Leroux‐Roels, Geert; Geysen, Dirk; Guisez, Yves

doi:10.1128/cvi.00382-07

Cited by 8 publications

(2 citation statements)

References 71 publications

(51 reference statements)

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“…Intraperitoneal immunization with the HBc VLP induced a strong, mixed Th1/Th2 response. In contrast, oral administration of the HBc VLP generated a high humoral response with mainly IgG2a antibodies, directing toward a Th1 response which is essential in the control of intracellular pathogens . In addition, the intranasal monovalent adjuvanted Norwalk VLP vaccine was well tolerated and highly immunogenic …”

Section: Immunostimulation By Vlpsmentioning

confidence: 99%

Different applications of virus‐like particles in biology and medicine: Vaccination and delivery systems

2015

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Virus-like particles (VLPs) mimic the whole construct of virus particles devoid of viral genome as used in subunit vaccine design. VLPs can elicit efficient protective immunity as direct immunogens compared to soluble antigens co-administered with adjuvants in several booster injections. Up to now, several prokaryotic and eukaryotic systems such as insect, yeast, plant, and E. coli were used to express recombinant proteins, especially for VLP production. Recent studies are also generating VLPs in plants using different transient expression vectors for edible vaccines. VLPs and viral particles have been applied for different functions such as gene therapy, vaccination, nanotechnology, and diagnostics. Herein, we describe VLP production in different systems as well as its applications in biology and medicine.

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Section: Immunostimulation By Vlpsmentioning

confidence: 99%

Different applications of virus‐like particles in biology and medicine: Vaccination and delivery systems

2015

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“…The low cost, high purity, content uniformity, greater stability and ease of storage have presented niosomes as better alternatives to liposomes. Cholera toxin (CT) and its non toxic components cholera toxin B (CTB) have been important in the development of the mucosal route as a useful and easily accessible non-invasive way to induce immunity [10][11][12][13]. Langerhans cells (LCs) are found in the epidermis, almost directly under the stratum corneum.…”

Section: Introductionmentioning

confidence: 99%

Non-ionic surfactant vesicles mediated transcutaneous immunization against hepatitis B

Maheshwari

Pandey

Chaurasiya

et al. 2011

International Immunopharmacology

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Immunogenic properties of a lettuce-derived C4(V3)6 multiepitopic HIV protein

Govea‐Alonso

Rubio-Infante

García‐Hernández

et al. 2013

Planta

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Elicitation of broad humoral immune responses is a critical factor in the development of effective HIV vaccines. In an effort to develop low-cost candidate vaccines based on multiepitopic recombinant proteins, this study has been undertaken to assess and characterize the immunogenic properties of a lettuce-derived C4(V3)6 multiepitopic protein. This protein consists of V3 loops corresponding to five different HIV isolates, including MN, IIIB, RF, CC, and RU. In this study, both Escherichia coli and lettuce-derived C4(V3)6 have elicited local and systemic immune responses when orally administered to BALB/c mice. More importantly, lettuce-derived C4(V3)6 has shown a higher immunogenic potential than that of E. coli-derived C4(V3)6. Moreover, when reactivity of sera from mice immunized with C4(V3)6 are compared with those elicited by a chimeric protein carrying a single V3 sequence, broader responses have been observed. The lettuce-derived C4(V3)6 has elicited antibodies with positive reactivity against V3 loops from isolates MN, RF, and CC. In addition, splenocyte proliferation assays indicate that significant T-helper responses are induced by the C4(V3)6 immunogen. Taken together, these findings account for the observed elicitation of broader humoral responses by the C4(V3)6 multiepitopic protein. Moreover, they provide further validation for the production of multiepitopic vaccines in plant cells as this serves not only as a low-cost expression system, but also as an effective delivery vehicle for orally administered immunogens.

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Comparison of Serum Humoral Responses Induced by Oral Immunization with the Hepatitis B Virus Core Antigen and the Cholera Toxin B Subunit

Cited by 8 publications

References 71 publications

Different applications of virus‐like particles in biology and medicine: Vaccination and delivery systems

Different applications of virus‐like particles in biology and medicine: Vaccination and delivery systems

Non-ionic surfactant vesicles mediated transcutaneous immunization against hepatitis B

Immunogenic properties of a lettuce-derived C4(V3)6 multiepitopic HIV protein

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