Comparison of serology, culture, and PCR for detection of brucellosis in slaughtered camels in Iran

Ghorbani, Azam; Khorasgani, Mohammad Rabbani; Zarkesh-Esfahani, Hamid; Sharifiyazdi, Hassan; Kashani, Ali Dehghan; Emami, Hamid

doi:10.1007/s00580-012-1499-1

Cited by 17 publications

(22 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The camel is a domestic mammal which due to its physiological attributes is suitable for use in climatic extremes. Diseases, poor nutrition, and traditional management systems have restricted their full utilization [1,2]. Even though several pastoral groups and communities all over the world depend on camels for their livelihood, the health status of camels has not yet received proper attention from researchers and scientists.…”

Section: Introductionmentioning

confidence: 99%

“…Camel brucellosis is caused by Gram-negative coccobacilli bacteria of the genus Brucella and is characterized by lesions of lymph nodes and joint capsules, orchitis and epididymitis, infl ammation of the uterus, abortion, and reduced fertility. Also, many infected camels are silent carriers of brucellosis [1][2][3]. Brucellosis remains to be the main zoonosis and is found globally.…”

Section: Introductionmentioning

confidence: 99%

“…Primarily, the camel may act as a reservoir for the dissemination of contaminated secretions to other domestic animals and humans. Secondly, in several nations, no formal surveillance and eradication programs for camel brucellosis have been proposed [2,3]. In some developed nations brucellosis is well controlled, however in Africa, Asia, South and Central America, and the Middle East, the clinical disease is still present among individual owners of camels.…”

Section: Introductionmentioning

confidence: 99%

“…The eradication of brucellosis is an essential step to control the disease in humans [2]. Cattle, goat, sheep, camels and other livestock may be infected and transmit the disease to human populations.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, pastoralists in endemic areas are at high risk of infection by Brucella species [5]. Brucellosis of camels in Iran has been studied mainly by serological methods, without isolation of the causative agent [2].…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Molecular Study of the Prevalence of Brucella Abortus and Brucella Melitensis in the Blood and Lymph Node Samples of Slaughtered Camels by Polymerase Chain Reaction (PCR) in Iran

et al. 2014

View full text Add to dashboard Cite

Brucellosis is a zoonotic disease which is characterized by reduced fertility and abortion in several species of animals, as well as humans. Camel brucellosis is caused by Brucella abortus and Brucella melitensis. To overcome the limitations posed by other techniques such as culture and serology, a sensitive technique (PCR) was employed for the detection of brucellosis in 123 camels. Findings from this PCR study indicated a total of 11.38% of blood samples as positive for Brucella spp. and 13.01% of the lymph node samples were positive for Brucella spp. In this study, 5 out of 123 (4.065%) and 3 out of 123 (2.439%) camel blood samples were positive for B. abortus and B. melitensis, respectively. Also, 4 out of 123 (3.252%) and 2 out of 123 (1.626%) camel lymph node samples were positive for B. abortus and B. melitensis, respectively. Young camels were the most commonly infected age group, while adult camels were the less often infected age group. Also, higher prevalence of brucellosis was observed in female camels. These results have indicated that PCR is a sensitive technique which could be used as a confi rmatory test for the detection of brucellosis in live camels, at the same time with the lowest risk of infection of laboratory personnel. The obtained results suggest that control and eradication programs for Brucella spp. infection seem to be necessary in camels. Our fi ndings support the power of PCR test for Brucella spp. detection in the blood and lymph node samples and it could be easily used for routine diagnosis.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Molecular Study of the Prevalence of Brucella Abortus and Brucella Melitensis in the Blood and Lymph Node Samples of Slaughtered Camels by Polymerase Chain Reaction (PCR) in Iran

et al. 2014

View full text Add to dashboard Cite

show abstract

Zoonotic implications of camel diseases in Iran

Mohammadpour

Champour

Tuteja

et al. 2020

Veterinary Medicine & Sci

View full text Add to dashboard Cite

Approximately 60% of all human pathogens and 75% of emerging infectious diseases are zoonotic (of animal origin). Camel zoonotic diseases can be encountered in all camel‐rearing countries. In this article, all studies carried out on camel zoonotic diseases in Iran are reviewed to show the importance of camels for public health in this country. More than 900 published documents were systematically searched to find relevant studies from 1,890 until late 2018. The collected articles were classified according to the aetiological agents. In this study, 19 important zoonotic diseases were reported among Iranian camels including listeriosis, leptospirosis, plague, Q fever, brucellosis, campylobacteriosis, tuberculosis, pasteurellosis, clostridiosis, salmonellosis, Escherichia coli infections, rabies, camelpox, Middle East respiratory syndrome coronavirus, Crimean‐Congo haemorrhagic fever, echinococcosis, cryptosporidiosis, toxoplasmosis and dermatophytosis, most of which belong to bacterial, viral, parasitic and fungal pathogens, respectively. Results show that camels are one of the most important sources of infections and diseases in human; therefore, continuous monitoring and inspection programs are necessary to prevent the outbreak of zoonotic diseases caused by this animal in humans.

show abstract

Pathological and diagnostic investigations of abortions and neonatal mortality associated with natural infection of Brucella abortus in dromedary camels

et al. 2016

View full text Add to dashboard Cite

Comparison of serology, culture, and PCR for detection of brucellosis in slaughtered camels in Iran

Cited by 17 publications

References 25 publications

Molecular Study of the Prevalence of Brucella Abortus and Brucella Melitensis in the Blood and Lymph Node Samples of Slaughtered Camels by Polymerase Chain Reaction (PCR) in Iran

Molecular Study of the Prevalence of Brucella Abortus and Brucella Melitensis in the Blood and Lymph Node Samples of Slaughtered Camels by Polymerase Chain Reaction (PCR) in Iran

Zoonotic implications of camel diseases in Iran

Pathological and diagnostic investigations of abortions and neonatal mortality associated with natural infection of Brucella abortus in dromedary camels

Contact Info

Product

Resources

About