Comparison of serological and DNA PCR-SSP typing results for HLA-A and HLA-B in 421 black individuals

Mytilineos, Joannis; Lempert, M.; Scherer, Sabine; Schwarz, V.; Opelz, Gerhard

doi:10.1016/s0198-8859(98)00048-2

Cited by 32 publications

(21 citation statements)

References 16 publications

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“…[24] Also Mytilineos et al [25] found 4.8% mismatch ratio for HLA-A and 13.8% for HLA-B. Another group compared the samples from cord blood and they expressed 13.8% error rate for serologic methods.…”

Section: Discussionmentioning

confidence: 99%

Association Between Human Leukocyte Antigen ( HLA ) With Chronic Renal Disease

Kodaz¹

2017

EJMI

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Section: Discussionmentioning

confidence: 99%

Association Between Human Leukocyte Antigen ( HLA ) With Chronic Renal Disease

Kodaz¹

2017

EJMI

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“…Overall, 36.3% of African Americans showed either an HLA-A or HLA-B discrepancy (1056). In the same study, only 8.5% of Caucasian kidney donors and recipients were mistyped.…”

Section: Histocompatibilitymentioning

confidence: 76%

“…Molecularly defined alleles or subtypes may be the best strategy to eliminate inconsistencies (1054). Comparisons of serological and molecularly defined HLA class typing have demonstrated that mistyping occurs (1055,1056). The more accurate molecular typing may be necessary to distinguish between a zero mismatch and true 6-antigen matched grafts.…”

Section: Histocompatibilitymentioning

confidence: 99%

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The Evaluation of the Renal Transplant Candidates: Clinical Practice Guidelines

2001

American Journal of Transplantation

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“…3,5,6 The limitations of HLA-AB serology have been first analyzed in the organ transplant field with 5-25% discrepancy rates in class I typings. [7][8][9][10] In particular, a 25% discrepancy rate for HLA-B serological homozygotes has been reported. 9 More recently, a combined serology/polymerase chain reaction-sequence specific primers (PCR-SSP) analysis of donors from the Tobias Registry (Sweden) showed an overall 3% discrepancy rate, but among homozygotes, 12% for HLA-A and 19% for HLA-B discrepancies, respectively, were observed.…”

mentioning

confidence: 99%

Quality control of a national bone marrow donor registry: results of a pilot study and proposal for a standardized approach

Stadelmann

Chapuis

et al. 2003

Bone Marrow Transplant

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Summary:Unrelated hematopoietic stem cell transplantation (HSCT) is a recognized therapy for hematological diseases and over 8 million HLA-typed donors are ready to donate. Increased international exchanges and rapid requests through the Bone Marrow Donor Worldwide (BMDW) ask for standardized quality assurance. Since no such standards have been established to date, we tested a pilot program in order to evaluate donor availability and quality of HLA typing of the Swiss Registry.The 18500 donors of the registry have been analyzed by serology for HLA-AB and by molecular typing for HLA-DR. Through three successive annual quality control (QC) exercises, a total of 114 donor requests were sent to 13 blood transfusion centers responsible for donor recruitment asking for a blood sample. Donors were randomly selected according to recruitment periods (1988-1993; 1994-1997; 1998-2000), and to homozygosity for HLA-A and/or -B antigens. An additional 80 frozen blood samples from the repository corresponding to the three periods (n ¼ 26) and to the 2001 period (n ¼ 54) were also included in the HLA study. HLA-AB typings were done by polymerase chain reaction-sequence specific primers (PCR-SSP) and all discrepancies were retyped. The results showed that 79 samples provided by 69.3% of the requested donors were received within 14 days, and 19 samples (16.7%) were received in 414 days. Altogether, an 86% rate of donor availability was observed, independent of the recruitment period. Among the requested donors, 16 (14%) were not available: for medical reasons (two), for personal reasons (eight), for loss (one), and for an unknown reason (five). The HLA-A/B DNA typing results of 166 homozygous and 12 heterozygous blood samples showed that 437/439 (99.5%) of the assigned A/B antigens were correct.However in 36/178 donors (20.2%) an HLA-A or -B antigen had been missed (34 donors) or misassigned (two donors) by serology, with a decreasing discrepancy rate of 30% (1988)(1989)(1990)(1991)(1992)(1993) to 18.5% in 2001. Assuming that HLA-A or -B homozygotes are found in 10-15% of the donors and that correct assignments have been observed in nearly 100% of the donors, an overall error rate of 4-5% would be expected for the national registry HLA-AB typing. These data show that standardized quality control for donor availability and HLA typing is feasible, and we propose that this model could be applied to the registries participating in bone marrow donor worldwide. Bone Marrow Transplantation (2003) 32, 623-627. doi:10.1038/sj.bmt.1704229 Keywords: bone marrow donor registry; quality control; donor availability; HLA serology; typing discrepancies; homozygotes Allogeneic hematopoietic stem cell transplantation (HSCT) is a recognized curative therapy for a number of hematological diseases and one-third of allogeneic transplants are now performed with HSC from an unrelated donor. 1 More than 8 million HLA-typed donors from 51 bone marrow and 29 cord blood donor registries are available through bone marrow donor worldwide (BMDW), allowing opt...

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Comparison of serological and DNA PCR-SSP typing results for HLA-A and HLA-B in 421 black individuals

Cited by 32 publications

References 16 publications

Association Between Human Leukocyte Antigen ( HLA ) With Chronic Renal Disease

Association Between Human Leukocyte Antigen ( HLA ) With Chronic Renal Disease

The Evaluation of the Renal Transplant Candidates: Clinical Practice Guidelines

Quality control of a national bone marrow donor registry: results of a pilot study and proposal for a standardized approach

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