Comparison of Serological and Culture Plate Methods for Detecting Species of Phytophthora, Pythium, and Rhizoctonia in Ornamental Plants

MacDonald, J. D.

doi:10.1094/pd-74-0655

Cited by 53 publications

(31 citation statements)

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“…are important for disease management especially for quarantine purposes and during pre-planting restricting the spread of Phytophthora disease through plant materials (MacDonald et al 1990). Traditionally, production of oogonia, antheridia and oospores (sexual spores) and the morphology of asexual spores (zoosporangium and chlamydospores) produced by Phytophthora spp.…”

Section: Introductionmentioning

confidence: 99%

Identification of Phytophthora spp. from Perennial Crops in Malaysia, Its Pathogenicity and Cross-pathogenicity

Latifah¹,

Sijam²,

Abidin³

et al. 2018

JSM

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Section: Introductionmentioning

confidence: 99%

Identification of Phytophthora spp. from Perennial Crops in Malaysia, Its Pathogenicity and Cross-pathogenicity

Latifah¹,

Sijam²,

Abidin³

et al. 2018

JSM

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“…So far, detection methods have included visual examination based on the taxonomic key of Stamps et al (1990) and isolation in selective media, but these traditional methods are time-consuming, labour-intensive and very often they require extensive knowledge of fungal taxonomy. Serological techniques have also been developed in different Phytophthora species (Jones and Shew, 1988;McDonald et al, 1990;Grote and Gabler, 1999), but the lack of specificity of some antibodies and the necessity of obtaining monoclonal antibodies complicate the technique (Bonants et al, 1997). Molecular methods and in particular the polymerase chain reaction (PCR) have been successful in identifying and detecting different fungal plant pathogens Schena et al, 2002 a, b;Ippolito et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

Development of specific PCR primers for identification and detection of Phytophthora capsici Leon

Silvar

Duncan²,

Cooke³

et al. 2005

Eur J Plant Pathol

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A PCR-based method was developed for the identification and detection of Phytophthora capsici in pepper plants. Three PCR primers (CAPFW, CAPRV1 and CAPRV2) specific for P. capsici were designed based on the sequence of its internal transcribed spacer regions. CAPFW/CAPRV1 amplify a 452 bp product from P. capsici DNA whereas CAPFW/CAPRV2 a 595 bp fragment; neither set amplifies DNA from pepper or several fungi pathogenic to pepper. In conventional (single-round) PCR, the limit of detection was 5 pg DNA for both primer sets, whereas in nested PCR the detection limit for both was of 0.5 fg. However, when the dilution series of target DNA were spiked with plant DNA, amplification declined two-fold in both conventional and nested PCR. The CAPFW/CAPRV2 set in conventional PCR was used to detect P. capsici DNA in inoculated plants. Detection occurred as soon as 8 h post-inoculation in stem samples from infected but still symptomless plants. The method was also tested to detect fungal DNA in infected soils.

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“…In a number of host-pathogen interactions, resistance is also expressed in tissue culture under proper cultural conditions (Ingram 1969;Dezoetoan et al 1982;Mauch-Mane et al 1989). Many immunological techniques have also been described for specific identification, quantification and localization of plant pathogens (McDonald et al 1990;Harrison et al 1991). Enzyme-linked immunosorbent assay (ELISA) has been widely applied in plant pathology, including detection of fungal pathogens in host plant tissues.…”

Section: Discussionmentioning

confidence: 99%

Immunological characterization of Karnal bunt resistance by differential response of pathogen restriction in a dual culture system

Sethi

Garg

Singh

et al. 2005

Food and Agricultural Immunology

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2005)Immunological characterization of Karnal bunt resistance by differential response of pathogen restriction in a dual culture system, Food and Agricultural Immunology, 16:1, 59-71, AbstractFor monitoring disease progression and evaluating Karnal bunt resistance dual cultures were established on callus induced from mature embryos of eight host varieties and two non-host varieties inoculated with a sporidial suspension of a virulent strain of Tilletia indica pathogen. Histochemical studies of dual-cultured calluses showed fungal mycelial growth and formation of chlamydospore-like structures that were more profuse in susceptible lines than in resistant and non-host lines. The formation of a zone of hollowness, i.e. a gap between mycelia and host tissue, an indication of pathogen restriction, was seen in resistant hosts and non-hosts but not in susceptible hosts. Detection and quantification of mycelia and teliospores in dual-cultured calluses by immunoassays revealed remarkable difference in the deposition of mycelia and teliospores-like structures. Susceptible calluses supported more abundant mycelial growth than the resistant and non-host co-cultured calluses based on the binding reaction of polyclonal antibodies.

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Comparison of Serological and Culture Plate Methods for Detecting Species of Phytophthora, Pythium, and Rhizoctonia in Ornamental Plants

Cited by 53 publications

References 0 publications

Identification of Phytophthora spp. from Perennial Crops in Malaysia, Its Pathogenicity and Cross-pathogenicity

Identification of Phytophthora spp. from Perennial Crops in Malaysia, Its Pathogenicity and Cross-pathogenicity

Development of specific PCR primers for identification and detection of Phytophthora capsici Leon

Immunological characterization of Karnal bunt resistance by differential response of pathogen restriction in a dual culture system

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