Comparison of Sensitivity of Sucrose Gradient, Wet Mount and Formalin - Ether in Detecting Protozoan Giardia lamblia in Stool Specimens of BALB/c Mice

Elmi, Taher; Gholami, Shirzad; Esboei, Bahman Rahimi; Garaili, Zarah; Najm, Mehdi; Tabatabaie, Fatemeh

doi:10.22207/jpam.11.1.14

Cited by 11 publications

(6 citation statements)

References 12 publications

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“… 34 However, molecular methods are preferred for conducting research activities because they have higher sensitivity and specificity and interpretation of the results is easier. 35 , 36 Accordingly, the pooled prevalence provided by molecular methods could be closer to the true prevalence. For a deeper understanding of this issue, it is necessary for researchers to use molecular methods in addition to microscopy methods.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of Giardia duodenalis among Asian children: a systematic review and meta-analysis

Kalavani,

Matin,

Rahmanian

et al. 2023

International Health

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Giardia duodenalis is one of the major causes of diarrhea among children. We performed a systematic review and meta-analysis to assess the prevalence of G. duodenalis and associated risk factors among Asian children. We searched online databases (PubMed, Scopus and Web of Science) and Google Scholar search engine for studies published from 1 January 2000 to 15 March 2022 that measured the prevalence of G. duodenalis among Asian children. Accordingly, the pooled prevalence and 95% CIs were estimated using a random-effects meta-analysis model for the included studies. A total of 182 articles from 22 Asian countries met the inclusion criteria. The pooled prevalence of G. duodenalis infection among Asian children was estimated as 15.1% (95% CI 14.1 to 16%). The highest and lowest pooled prevalence values of G. duodenalis infection were estimated for Tajikistan and China as 26.4% (95% CI 22.9 to 30%) and 0.6% (95% CI 0.001 to 1.02%), respectively. The infection had a higher prevalence in males than in females (OR=1.24; 95% CI 1.16 to 1.31; p<0.001), which was statistically significant. Giardiasis is common among Asian children, hence, a prevention and control scheme of this protozoan in children should be considered by health officials and health policymakers, especially in Asian countries where the prevalence is highest.

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Section: Discussionmentioning

confidence: 99%

Prevalence of Giardia duodenalis among Asian children: a systematic review and meta-analysis

Kalavani,

Matin,

Rahmanian

et al. 2023

International Health

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“…G iardia lamblia cysts were isolated from stool samples of patients who are suffering from giardiasis and all samples were processed ordinarily within 48 h after excretion. A highly purified cyst suspension was achieved by the sucrose 0.85 M flotation method with a simplified sucrose gradient method that is previously described (Elmi T et al ., 2017). Purified cysts were resuspended in normal saline and stored at 4 °C for a maximum of 3 days prior to use.…”

Section: Methodsmentioning

confidence: 99%

Effects of some natural products from fungal and herbal sources on Giardia lamblia in vivo

et al. 2019

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Giardia lamblia (G. lamblia) is the most widely known protozoan parasite that causes human gastrointestinal infection worldwide. Some natural compounds exhibited pivotal effects against different infectious diseases. In this research, the antigiardial activity and cytotoxicity of fungal chitosan, nano-chitosan, Rhamnus cathartica (R. cathartica) and emodin were evaluated in Balb/c mice. Genotyping of G. lamblia was assessed by PCR-RFLP technique. Different concentrations of mentioned compounds were used to check their antigiardial and cytotoxicity effects on human intestinal epithelial cells (HT-29) after 24, 48 and 72 h. The G. lamblia strain used in the current work was genotyped and revealed as an AII assemblage. All the concentration showed acceptable activity against G. lamblia cysts and trophozoites in comparison to the negative and positive controls (furazolidone and metronidazole) in vitro (P < 0.05). Giardia lamblia cysts were susceptible after treatment in all experiments in vivo in comparison to negative control (P < 0.05). Approximately, in most of the concentration, nano-chitosan and emodin were more effective than chitosan and R. cathartica, respectively (P < 0.05). The effects of exposure times in antigiardial and cytotoxicity effects were not statistically significant (P > 0.05). The maximum mortality rate (100%) was achieved at 100 and 50 µg kg−1 concentrations after 48 and 72 h of exposure time, respectively. Our results provide significant information about the new antigiardial agent and proposed the nano-chitosan and emodin for the development of new drugs against G. lamblia in the future.

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“…Sometimes, trophozoite stage can be detected from diarrheic samples but immediate microscopic observation is required to observe trophozoites due to their characteristic movement. Alternatively, microscopic observation of the cyst is preferred over trophozoite detection which is mostly present in faeces (Elmi et al 2017). Staining is usually done to observe the cyst under microscope.…”

Section: Microscopic Examinationmentioning

confidence: 99%

Giardiasis in Human and Animals

2021

Int J Vet Sci

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Comparison of Sensitivity of Sucrose Gradient, Wet Mount and Formalin - Ether in Detecting Protozoan Giardia lamblia in Stool Specimens of BALB/c Mice

Cited by 11 publications

References 12 publications

Prevalence of Giardia duodenalis among Asian children: a systematic review and meta-analysis

Prevalence of Giardia duodenalis among Asian children: a systematic review and meta-analysis

Effects of some natural products from fungal and herbal sources on Giardia lamblia in vivo

Giardiasis in Human and Animals

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