Comparison of semiquantitative test strips, urine protein electrophoresis, and an immunoturbidimetric assay for measuring microalbuminuria in dogs

Murgier, Philippe; Jakins, Amanda; Bexfield, Nick; Archer, Joy

doi:10.1111/j.1939-165x.2009.00176.x

Cited by 14 publications

(21 citation statements)

References 18 publications

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“…A study comparing semiquantitative test strips and quantitative albumin assessment indicated that 29% of dogs with measurable MA of > 1.4 mg/L were negative using the semiquantitative test strips, whereas 71% were positive. Using 10 mg/L as a cut‐off for MA, the test strips had false‐negative and false‐positive rates of 9 and 8%, respectively …”

Section: Discussionmentioning

confidence: 99%

Arterial Blood Pressure, Proteinuria, and Renal Histopathology in Clinically Healthy Retired Racing Greyhounds

Surman

Couto

DiBartola

et al. 2012

Veterinary Internal Medicne

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Background: Physiologic peculiarities of Greyhounds as compared to other dogs make interpretation of laboratory results in this breed challenging for veterinarians. Hypertension in retired racing Greyhounds (RRG) can contribute to microalbuminuria (MA), overt proteinuria, and renal histologic lesions.Objectives: To evaluate clinicopathologic findings, hemodynamic status, and renal histology in a population of healthy RRG.Animals: RRG presented to Ohio State University College of Veterinary Medicine for inclusion in a spay and neuter program.Methods: Cross-sectional study. RRG were classified as normotensive (<160 mmHg) or hypertensive (>160 mmHg) based on blood pressure (BP) determinations using Doppler and oscillometric methods. Of the dogs evaluated, 62% (n = 29) were hypertensive and 38% (n = 18) were normotensive. Health status was evaluated using routine clinicopathologic tests (CBC, serum biochemistry, urinalysis) as well as evaluation of fractional excretion of electrolytes and MA determinations. Adequate renal biopsy specimens (n = 15) were evaluated using light, immunofluoresence, and electron microscopy.Results: All serum biochemistry results were normal in 45/49 dogs, but MA was more common in hypertensive (84% positive for MA) as compared with normotensive (18% positive for MA) RRG. Observed renal lesions were mild and renal biopsy scores were low in this sample of RRG.Conclusions: Hypertension is common in RRG and might be breed-related. It is associated with MA, but observed renal lesions are mild. Whether or not hypertension and MA in RRG leads to progressive renal damage requires longitudinal study.

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Section: Discussionmentioning

confidence: 99%

Arterial Blood Pressure, Proteinuria, and Renal Histopathology in Clinically Healthy Retired Racing Greyhounds

Surman

Couto

DiBartola

et al. 2012

Veterinary Internal Medicne

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“…In reports on uALB, uRBP, or uNAG in canine or feline urine, cystocentesis as well as catheterization and midstream voided urine have been used. 12,19,30 In contrast to voided samples, cystocentesis avoids contamination with lower urinary tract epithelial cells, proteases, and bacteria that may affect urinary markers. 22 When performing cystocentesis, care must be taken to avoid peripheral blood contamination leading to grossly discolored urine samples, since this may cause a falsely increased urinary albumin concentration.…”

Section: Discussionmentioning

confidence: 99%

Effect of Sampling Method and Storage Conditions on Albumin, Retinol-Binding Protein, And N-Acetyl-β-D-Glucosaminidase Concentrations in Canine Urine Samples

et al. 2010

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Abstract. Urinary markers for renal dysfunction are gaining interest, but effects of sampling method, storage conditions, and urinary tract inflammation or infection on these markers are unclear. Therefore, the objectives of the current study were to determine the difference in urinary albumin (uALB), urinary retinolbinding protein (uRBP), and urinary N-acetyl-b-D-glucosaminidase (uNAG) concentrations in cystocentesis and voided samples and to investigate concentration changes after storage at 220uC and at 280uC. Effects of a protease inhibitor were also assessed in samples stored at 280uC for 12 months. In a pilot experiment, influence of in vitro hematuria, pyuria, and bacteriuria on the urinary markers was evaluated. A mixed model was used to calculate mean differences and 95% confidence intervals. Urinary ALB, uNAG, and uRBP concentrations were similar in voided and cystocentesis samples. After storage for 4 months at 220uC, uALB concentration was not affected, and uRBP concentration showed a mild and clinically irrelevant decrease, whereas uNAG activity was significantly lower compared with fresh samples. After storage for 12 months at 280uC, uALB and uRBP concentrations did not differ from fresh samples, but uNAG activity was severely decreased. Protease inhibitor addition did not preserve uNAG activity. Experimental hematuria, pyuria, and bacteriuria did not seem to affect urinary markers, although further research is needed.

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“…A calibration of the human nephelometric assay (BNII system, Kit N AS ALB, Siemens Healthcare) was deemed necessary because the monoclonal antibody against human albumin used in the assay shared only a partial cross-reactivity with canine albumin (Gentilini et al, 2005;Murgier et al, 2009). A calibration curve was constructed by diluting a solution of purified canine albumin (Canine Albumin Protein ab119814, Abcam) to concentrations varying between 1.6 and 100 mg/dL using 0.9% saline.…”

Section: Analysesmentioning

confidence: 99%

Evaluation of the effect of oral omeprazole on canine cerebrospinal fluid production: A pilot study

Girod

Allerton

Gommeren

et al. 2016

The Veterinary Journal

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A B S T R A C TAdministration of omeprazole by ventriculo-cisternal perfusion or intravenously has been shown to decrease cerebrospinal fluid (CSF) production in dogs and rabbits. Oral omeprazole has consequently been recommended to reduce CSF production in dogs with conditions in which clinical signs may be attributable to an accumulation of CSF in the central nervous system (e.g. hydrocephalus, syringomyelia). The albumin quotient (QAlb), the ratio between CSF and serum albumin concentration, has been proposed as a reliable means to evaluate CSF production; decreasing CSF production should cause an increase in QAlb.The aims of this study were to assess the effect of oral administration of omeprazole on QAlb in dogs and to compare two methods to assess CSF albumin concentration. Fifteen healthy Beagle dogs received omeprazole (1.2 mg/kg/day) orally for 14 days; CSF and blood were obtained before and after treatment. CSF albumin concentrations were evaluated by nephelometry and high-resolution protein electrophoresis. Regardless of the method used for measuring albumin, QAlb did not change significantly following oral omeprazole administration, suggesting that CSF production in healthy dogs may not be affected by chronic oral therapy with omeprazole.

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Comparison of semiquantitative test strips, urine protein electrophoresis, and an immunoturbidimetric assay for measuring microalbuminuria in dogs

Abstract: UPE and semiquantitative test strips are less accurate than the automated immunoturbidimetric method for the measurement of albumin in canine urine.

Cited by 14 publications

References 18 publications

Arterial Blood Pressure, Proteinuria, and Renal Histopathology in Clinically Healthy Retired Racing Greyhounds

Arterial Blood Pressure, Proteinuria, and Renal Histopathology in Clinically Healthy Retired Racing Greyhounds

Effect of Sampling Method and Storage Conditions on Albumin, Retinol-Binding Protein, And N-Acetyl-β-D-Glucosaminidase Concentrations in Canine Urine Samples

Evaluation of the effect of oral omeprazole on canine cerebrospinal fluid production: A pilot study

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