Comparison of semiautomatic digitizer-tablet and simple point counting performance in morphometry

Jørgen, H; Gundersen, Gregory W.; Boysen, Morten; Reith, Albrecht

doi:10.1007/bf02892580

Cited by 133 publications

(58 citation statements)

References 4 publications

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“…Estimation of the section areas need not be a laborious task. Conventional planimetric methods can be replaced by the stereological method of point counting, which is unbiased and far more efficient than planimetry (Mathieu et al, 1981;Gundersen et al, 1981). Further, the statistical error of the Cavalieri estimator can be assessed, and its two components due to sectioning and point counting can be estimated.…”

Section: Introductionmentioning

confidence: 99%

Analysis of cardiac function by MRI and stereology

Roberts

Cruz‐Orive

Bourne

et al. 1997

Journal of Microscopy

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SummaryDesign-based stereology and phase contrast magnetic resonance imaging (MRI) were combined to monitor changes in the volume of the four chambers of the human heart during the cardiac cycle. The data set consisted of 18 adjacent slices (or 'scanning levels') of 0 . 5 cm thickness, perpendicular to the long axis of the body, and encompassing the whole heart of a healthy volunteer. At each scanning level, a cardiac gated MR image was obtained at each of 16 equally spaced time frames within the cardiac cycle. Given stationarity with respect to time, absence of image artefacts and appropriate definition of chamber boundaries, for each time frame unbiased estimates of total blood volume in the relevant heart chambers were efficiently obtained using the Cavalieri method and point counting. Combined with a proper MRI acquisition, modern stereological methods constitute an efficient and reliable tool to quantify cardiac function noninvasively.

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Section: Introductionmentioning

confidence: 99%

Analysis of cardiac function by MRI and stereology

Roberts

Cruz‐Orive

Bourne

et al. 1997

Journal of Microscopy

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show abstract

“…The method has been refined and modified in a series of applications involving invasive and noninvasive scanning (Gundersen and Jensen 1987;Mayhew and Olsen 1991;Pakkenberg et al 1989). According to Cavalieri principle, the volume of arbitrarily complex structures can be estimated from the sum of parallel areas separated by a known distance, provided that the set of sections is positioned at random with respect to the chosen axis (Gundersen and Jensen 1987). Profile areas are most efficiently estimated by point counting (Gundersen et al 1981;Mathieu et al 1981).…”

Section: Theoretical Background Of the Cavalieri Stereological Methodsmentioning

confidence: 99%

“…According to Cavalieri principle, the volume of arbitrarily complex structures can be estimated from the sum of parallel areas separated by a known distance, provided that the set of sections is positioned at random with respect to the chosen axis (Gundersen and Jensen 1987). Profile areas are most efficiently estimated by point counting (Gundersen et al 1981;Mathieu et al 1981). The Cavalieri method consists of the following steps:…”

Section: Theoretical Background Of the Cavalieri Stereological Methodsmentioning

confidence: 99%

“…Scan or physically cut the object in a series of parallel planes a distance T apart, the first plane starting randomly in the interval 0 to T. 2. The sectional area of the object cut can be efficiently estimated with the point-counting method (Gundersen et al 1981;Mathieu et al 1981). The point-counting method consists of randomly (conducted by computer) superimposing onto each section a test lattice overlay bearing a systematic arrangement of test points.…”

Section: Theoretical Background Of the Cavalieri Stereological Methodsmentioning

confidence: 99%

“…The usual formula for estimating CE (standard error of the mean divided by the mean) is valid for independently derived observations. The present design was based on systematic random sampling design that is an unbiased sampling design and is more efficient than independent random sampling design (Gundersen and Jensen 1987). Therefore, the CE for the present design cannot be calculated in the usual manner.…”

Section: Statisticsmentioning

confidence: 99%

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Unbiased Stereological Estimation of the Spiral Ligament and Stria Vascularis Volumes in Aging and Ménière’s Disease Using Archival Human Temporal Bones

Ishiyama

Tokita

López

et al. 2006

JARO

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The present study applies the unbiased stereological technique-Cavalieri principle to measure the volumes of the stria vascularis (SV) and the spiral ligament (SL) using postmortem archival human temporal bones from normal young and older subjects and subjects with Ménière_s disease. Normative data was obtained from subjects without ages ranging from 15 to 84 years old who had no history of audiovestibular disease (N = 25). For comparison purposes, the normative specimens were divided into three groups: group 1 (n = 8) had ages ranging from 15 to 38 years old, average age = 23.9; group 2 (n = 8) had ages ranging from 51 to 59 years old, average age = 55.1; group 3 (n = 9) had ages ranging from 64 to 84 years old, average age = 74.3. The average SV volume of group 3 (0.479 mm 3 ) was significantly lower than that of group 1 (0.705 mm 3 ) (p G 0.0005) and was significantly lower than that of group 2 (0.603 mm 3 ) (p = 0.01). The average SL volume of group 3 (8.42 mm 3 ) was significantly lower than that of group 1 (9.54 mm 3 ) (p G 0.05), but was not significantly lower than that of group 2 (8.58 mm 3 ). Five subjects with Ménière_s disease, confirmed by histopathological examination (ages ranging from 63 to 91 years old, average age = 73.4), were studied. The average SV volume in Ménière_s subjects (0.378 mm 3 ) was significantly lower than age-matched controls (p G 0.05). The average SL volume in Ménière_s subjects (7.01 mm 3 ) was also significantly lower than age-matched controls (p G 0.05). The SV and SL volumes were unaffected by gender. The present study demonstrates for the first time the use of the unbiased stereological technique-Cavalieri principle-as a reliable and efficient method to obtain volumetric estimates of the SV and the SL by using archival human temporal bone specimens.

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The Size of Lesions, or Point Counting as a Step Toward the Solution of the PASI Problem

Bahmer¹

1989

Arch Dermatol

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Comparison of semiautomatic digitizer-tablet and simple point counting performance in morphometry

Cited by 133 publications

References 4 publications

Analysis of cardiac function by MRI and stereology

Analysis of cardiac function by MRI and stereology

Unbiased Stereological Estimation of the Spiral Ligament and Stria Vascularis Volumes in Aging and Ménière’s Disease Using Archival Human Temporal Bones

The Size of Lesions, or Point Counting as a Step Toward the Solution of the PASI Problem

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