Comparison of semen collection techniques in goats

Memon, M.A.; Bretzlaff, K.N.; Ott, R.S.

doi:10.1016/0093-691x(86)90011-7

Cited by 32 publications

(16 citation statements)

References 10 publications

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“…Animals were likely stressed when being captured using anesthetic darts, maintained under general anesthesia and electroejaculated. This management has been previously reported to result in wide variability in sperm characteristics (Memon et al, 1986), and is highly stressful for the animals (Fumagalli et al, 2012). Considering that this isat least until now-the only available technique to obtain sperm from the pampas deer species, the low concentration, volume, and overall quality obtained would probably be a limitation for cryopreservation.…”

Section: Discussionmentioning

confidence: 98%

Sperm characterization and identification of sperm sub-populations in ejaculates from pampas deer (Ozotoceros bezoarticus)

Beracochea¹,

Gil²,

Sestelo³

et al. 2014

Animal Reproduction Science

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Section: Discussionmentioning

confidence: 98%

Sperm characterization and identification of sperm sub-populations in ejaculates from pampas deer (Ozotoceros bezoarticus)

Beracochea¹,

Gil²,

Sestelo³

et al. 2014

Animal Reproduction Science

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“…However, when the same parameters were compared in individuals, significant variations were found on most sperm parameters for the method of collection in the same male, but also significant variations were found between males collected by EE in parameters that were similar when semen was collected by AV. Earlier studies in ruminants have shown that sperm concentration is depressed by EE (Memon et al. 1986; Leon et al.…”

Section: Discussionmentioning

confidence: 99%

CASA Assessment of Kinematic Parameters of Ram Spermatozoa and their Relationship to Migration Efficiency in Ruminant Cervical Mucus

Robayo

Montenegro

Valdés

et al. 2008

Reprod Domestic Animals

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Sperm motility is an indicator of male fertility because of its importance for sperm migration through the female genital tract and for gamete interaction at fertilization. This study analyses the relationship between computer assisted semen analysis (CASA) motility patterns and sperm migration of rams in ruminant cervical mucus. In experiment 1, spermatozoa extended with sperm analysis medium (SAM) and seminal plasma were compared in terms of motility. In experiment 2, 56 semen samples were collected either with artificial vagina (AV) or electroejaculator to be compared in terms of motility performance. In experiment 3, 104 ejaculates collected by AV from 26 males were analysed via the CASA system to characterize their motility patterns. In experiment 4, ejaculates from pairs of rams (20 rams in total) were simultaneously assessed for mucus migration (ovine, caprine, bovine) and motility patterns to evaluate the correlations between both parameters. Semen collected by AV and extended in SAM allows the most reliable assessment for sperm motility. Ram spermatozoa move fast and follow a linear trajectory compared with other ruminants. Continuous line velocity (VCL) and average path velocity (VAP) are the only sperm kinematic parameters that presented significant positive correlations with the ability to migrate in sheep cervical mucus (p < 0.05). Continuous line velocity, VAP, straight line velocity and linearity are highly significantly related with migration efficiency in goat cervical mucus (p < 0.01) and only lateral head displacement is negatively related to sperm migration in bovine cervical mucus (p < 0.05). These results suggest that specific kinematic parameters confer the ability of spermatozoa to colonize and migrate through epithelial mucus with different rheological properties.

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“…Semen was collected from each animal twice a week, in different and non-consecutive days, using an artificial vagina (length = 20 cm and diameter = 4.5 cm) according to the method of Memon et al (1986). A total of six ejaculates were collected from each buck.…”

Section: Semen Collection Initial Evaluation and Poolingmentioning

confidence: 99%

Effect of egg yolk concentration and washing on sperm quality following cryopreservation in Barbari buck semen

Anand

Baghel

Yadav

2016

Journal of Applied Animal Research

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The present study was conducted to evaluate the effect of egg yolk concentration and washing on sperm quality in cryopreserved Barbari buck semen at higher dilution (100 × 10 6 cells/ml). Five healthy Barbari bucks of similar age and weight were selected as semen donor. Six ejaculates were collected from each buck during the experiment. Collected semen samples were pooled and diluted with Tris-based semen extender containing 6% glycerol and egg yolk according to the treatments -T-1, that is, 3% egg yolk, T-2, that is, 20% egg yolk and T-3, that is, 20% egg yolk after washing and cryopreserved using the slow freezing technique. Semen was evaluated at three different steps, namely, after dilution, after equilibration and after freeze-thawing. Significantly (p ≤ .01) higher values for motility and viability were observed in T-3 just after dilution while significantly (p ≤ .01) higher values for acrosomal integrity and pattern F (uncapacitated sperm) was observed in T-2. Significantly (p ≤ .01) higher values were observed in T-2 after equilibration and thawing. It may be concluded that incorporation of 20% egg yolk compared to 3% egg yolk or 20% egg yolk after washing in extender confer better cryoprotection to Barbari buck spermatozoa as reflected in semen quality parameters studied. ARTICLE HISTORY

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Comparison of semen collection techniques in goats

Cited by 32 publications

References 10 publications

Sperm characterization and identification of sperm sub-populations in ejaculates from pampas deer (Ozotoceros bezoarticus)

Sperm characterization and identification of sperm sub-populations in ejaculates from pampas deer (Ozotoceros bezoarticus)

CASA Assessment of Kinematic Parameters of Ram Spermatozoa and their Relationship to Migration Efficiency in Ruminant Cervical Mucus

Effect of egg yolk concentration and washing on sperm quality following cryopreservation in Barbari buck semen

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