Comparison of Self-Gated and Prospectively Triggered Fast Low Angle Shot (FLASH) Sequences for Contrast-Enhanced Magnetic Resonance Imaging of the Liver at 9.4 T in a Rat Model of Colorectal Cancer Metastases

Fries, Peter; Seidel, Roland; Müller, Andreas; Matthes, Kathrin; Denda, Gero; Maßmann, Alexander; Menger, Michael D.; Sperling, Jens; Morelli, John N.; Altmeyer, K.; Schneider, Günther; Buecker, Arno

doi:10.1097/rli.0b013e318294dd0e

Cited by 9 publications

(4 citation statements)

References 33 publications

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“…This technique obviates the need for respiration pads and ECG leads, thereby enhancing the throughput in animal studies. The self-gated MRI technique has been introduced for mouse cardiac and rat liver imaging [ 84 , 85 , 86 ], and for (mouse) lung MRI [ 5 ].…”

Section: The Multimodal Toolbox For Biomedical Lung Disease Researmentioning

confidence: 99%

From Mouse to Man and Back: Closing the Correlation Gap between Imaging and Histopathology for Lung Diseases

et al. 2020

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Lung diseases such as fibrosis, asthma, cystic fibrosis, infection and cancer are life-threatening conditions that slowly deteriorate quality of life and for which our diagnostic power is high, but our knowledge on etiology and/or effective treatment options still contains important gaps. In the context of day-to-day practice, clinical and preclinical studies, clinicians and basic researchers team up and continuously strive to increase insights into lung disease progression, diagnostic and treatment options. To unravel disease processes and to test novel therapeutic approaches, investigators typically rely on end-stage procedures such as serum analysis, cyto-/chemokine profiles and selective tissue histology from animal models. These techniques are useful but provide only a snapshot of disease processes that are essentially dynamic in time and space. Technology allowing evaluation of live animals repeatedly is indispensable to gain a better insight into the dynamics of lung disease progression and treatment effects. Computed tomography (CT) is a clinical diagnostic imaging technique that can have enormous benefits in a research context too. Yet, the implementation of imaging techniques in laboratories lags behind. In this review we want to showcase the integrated approaches and novel developments in imaging, lung functional testing and pathological techniques that are used to assess, diagnose, quantify and treat lung disease and that may be employed in research on patients and animals. Imaging approaches result in often novel anatomical and functional biomarkers, resulting in many advantages, such as better insight in disease progression and a reduction in the numbers of animals necessary. We here showcase integrated assessment of lung disease with imaging and histopathological technologies, applied to the example of lung fibrosis. Better integration of clinical and preclinical imaging technologies with pathology will ultimately result in improved clinical translation of (therapy) study results.

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Section: The Multimodal Toolbox For Biomedical Lung Disease Researmentioning

confidence: 99%

From Mouse to Man and Back: Closing the Correlation Gap between Imaging and Histopathology for Lung Diseases

et al. 2020

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“…An implemented navigator pulse scheme monitors the abdominal wall motion allowing for MRI under free respiration. 21 The unenhanced T1-weighted sequences were acquired twice, and mean as well as subtracted datasets were calculated for noise measurements. Thereafter, contrast agents were injected through a previously inserted tail vein catheter (27 gauge).…”

Section: In Vivo Magnetic Resonance Imagingmentioning

confidence: 99%

Evaluation of Gadopiclenol and P846, 2 High-Relaxivity Macrocyclic Magnetic Resonance Contrast Agents Without Protein Binding, in a Rodent Model of Hepatic Metastases

et al. 2019

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The aim of this study was to evaluate in vitro and in vivo the enhancement properties of experimental gadolinium (Gd)-based contrast agents (GBCAs) with different molecular weights and hydration numbers (P846 and gadopiclenol) compared with clinically approved low-molecular, extracellular agents (gadopentetate and gadoterate) at 9.4 T and to discuss influencing factors on r1 relaxivities. Methods and Materials: All experiments were performed with a 9.4 T animal scanner (Bruker, Germany). We performed relaxometry measurements for all contrast agents in human plasma at 37°C using an IR-RARE sequence. In addition, we compared P846 with gadopentetate and gadopiclenol with gadoterate intraindividually in rats with hepatic colorectal cancer metastases (n = 10 each) acquiring T1-weighted FLASH sequences before and at 10 consecutive time points during 20 minutes. After intravenous contrast agent application, signal-to-noise ratios (SNRs), contrast-to-noise ratios (CNRs), and lesion enhancement (LE) for liver parenchyma and tumors were calculated based on region of interest measurements. Results: Longitudinal relaxivities (r1) of the low-molecular agents were lower as compared with the experimental compounds. However, r1 of gadopentetate and gadoterate demonstrated only a moderate decrease of r1 at 9.4 T as compared with known data at lower field strengths (gadopentetate: r1 [at 9.4 T], 3.4 mM −1 s −1 /r1 [at 1.5 T], 4.1 mM −1 s −1 /gadoterate: r1 [at 9.4 T], 3.1 mM −1 s −1 /r1 [at 1.5 T], 3.6 mM −1 s −1 ). In contrast, r1 of P846 showed a marked reduction at 9.4 T compared with 1.5 T (P846: r1 [at 9.4 T], 6.4 mM −1 s −1 /r1 [at 1.5 T], 32 mM −1 s −1 ). Gadopiclenol provided the highest r1 in this study at 9.4 T and the drop of r1 as compared with lower field strength is less apparent (gadopiclenol: r1 [at 9.4 T], 8.7 mM −1 s −1 /r1 [at 1.5 T], 12.7 mM −1 s −1 ).In vivo, P846 and gadopiclenol showed significantly higher SNR, CNR, and LE as compared with the low-molecular control agents (mean ± SD; SNR liver [gadopentetate, 18.1 ± 1.

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“…Unfortunately, quantitative imaging in small animals can be challenging due to anesthetized respiration rates of 40 to 80 breaths per minute and heart rates of >200 beats per minute, causing blurring and ghosting artifacts that can result in quantification errors (4,5,(7)(8)(9)(10)(11). Unfortunately, prospective respiratory and/or cardiac gating to reduce the motion artifacts (9,(12)(13)(14)(15) may not be practical because it substantially can extend acquisition times (16). Intubation and significant motion restriction (11,17) also can help reduce motion artifacts but may cause increased mortality in animal models with advanced disease.…”

Section: Introductionmentioning

confidence: 99%

Regularly incremented phase encoding – MR fingerprinting (RIPE‐MRF) for enhanced motion artifact suppression in preclinical cartesian MR fingerprinting

Anderson

Wang

et al. 2017

Magnetic Resonance in Med

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Comparison of Self-Gated and Prospectively Triggered Fast Low Angle Shot (FLASH) Sequences for Contrast-Enhanced Magnetic Resonance Imaging of the Liver at 9.4 T in a Rat Model of Colorectal Cancer Metastases

Cited by 9 publications

References 33 publications

From Mouse to Man and Back: Closing the Correlation Gap between Imaging and Histopathology for Lung Diseases

From Mouse to Man and Back: Closing the Correlation Gap between Imaging and Histopathology for Lung Diseases

Evaluation of Gadopiclenol and P846, 2 High-Relaxivity Macrocyclic Magnetic Resonance Contrast Agents Without Protein Binding, in a Rodent Model of Hepatic Metastases

Regularly incremented phase encoding – MR fingerprinting (RIPE‐MRF) for enhanced motion artifact suppression in preclinical cartesian MR fingerprinting

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