COMPARISON OF SELECTIVE AND NONSELECTIVE ENRICHMENT MEDIA FOR THE ISOLATION OF <i>LISTERIA</i> SPECIES FROM RETAIL FOODS

Walsh, Des; Duffy, Geraldine; Sheridan, J.J.; Blair, I.S.; McDowell, D.A.

doi:10.1111/j.1745-4565.1998.tb00205.x

Cited by 22 publications

(25 citation statements)

References 26 publications

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“…Sub-lethally injured L. monocytogenes bacteria often have a slower growth rate, altered virulence characteristics and higher sensitivity to unfavourable conditions than their healthy counterparts (McCarthy 1991;Buncic and Avery 1996;Avery and Buncic 1997). One important consequence of this feature is that selective compounds such as antibiotics, often used in media for the specific isolation of this pathogen from foods, may kill the injured cells (Busch and Donnelly 1992;Sheridan et al 1994;Walsh et al 1998). Under suitable conditions, however, the same sub-lethally injured cells may repair the damage and regain normal growth and virulence potential (Busch and Donnelly 1992;Buncic and Avery 1996).…”

Section: Introductionmentioning

confidence: 99%

“…For example, freezing induces substantial cell wall and plasma membrane damage (El-Kest and Marth 1992), whereas heating and chlorine-based sanitizers do not affect these cell components but instead damage cellular proteins (Bunduki et al 1995). For effective resuscitation and detection of damaged cells, the specific types of injury caused by a given treatment must be understood and compensated for (Walsh et al 1998). Although an effective resuscitation medium (Busch and Donnelly 1992) has been developed that allows recovery of L. monocytogenes cells from heat-and freeze-stressed environments, it may not be equally effective for other types of sub-lethal injury.…”

Section: Introductionmentioning

confidence: 99%

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Physical and metabolic causes of sub-lethal damage in Listeria monocytogenes after long-term chilled storage at 4 oC

Dykes¹

1999

J Appl Microbiol

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S. 1999. Cells of Listeria monocytogenes display sub-lethal injury when subjected to long-term chill-storage in a nutrient-poor environment. The physical and metabolic causes of sub-lethal injury to two meat (L61 and L62) and two clinical (L98 and L99) L. monocytogenes strains chill-stored (4°C) for 4 weeks in phosphate-buffered saline at pH 7·0 and pH 5·5, and pH 5·5 in the presence of 0·3% potassium sorbate, were characterized. Causes of sub-lethal injury were determined by examining changes in the cell structure, leakage of nucleic acids and proteins from the cells, and cell recovery from injury in the presence of the metabolic inhibitors rifampicin, D-cycloserine, carbonyl cyanide m-chlorophenylhydrazone and chloramphenicol. Visible shrinkage of the cytoplasm and slight cell wall damage were apparent over the 4 week storage period by electron microscopy for all four strains and three storage conditions. By contrast, over the same storage period, only three of the strains (L62, L98 and L99) displayed slight leakage of cellular content in all three storage media, while one strain (L61) displayed greater leakage. The three strains also displayed similar storage mediadependent metabolic damage. For these strains, phosphate-buffered saline pH 5·5 caused the least damage and potassium sorbate, the most. Recovery experiments also indicated that at pH 5·5, the energy transduction system of these three strains remained undamaged, and that injury to the cell transcription machinery was greatest at pH 7·0. The fourth strain, L61, displayed less damage than the others but this was attributed to the death of the injured cell sub-population in this strain. In this study, damage to sub-lethally injured chill-stored L. monocytogenes was different from that caused by other agents, such as heat. Therefore, cells injured by chill-storage under starvation conditions may require novel protocols to assure their effective recovery.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Physical and metabolic causes of sub-lethal damage in Listeria monocytogenes after long-term chilled storage at 4 oC

Dykes¹

1999

J Appl Microbiol

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“…to interact with L. monocytogenes. Nonpathogenic species such as L. innocua and L. welshimeri have been found to be more common than L. monocytogenes in food processing environments and in foods and to grow faster than L. monocytogenes in foods and other media (2,8,19,30,36,47,49,54). These Listeria spp.…”

mentioning

confidence: 99%

Coselection of Cadmium and Benzalkonium Chloride Resistance in Conjugative Transfers from Nonpathogenic Listeria spp. to Other Listeriae

Katharios-Lanwermeyer

Rakić-Martinez

Elhanafi

et al. 2012

Appl Environ Microbiol

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Resistance to the quaternary ammonium disinfectant benzalkonium chloride (BC) may be an important contributor to the ability of Listeria spp. to persist in the processing plant environment. Although a plasmid-borne disinfectant resistance cassette (bcrABC) has been identified in Listeria monocytogenes, horizontal transfer of these genes has not been characterized. Nonpathogenic Listeria spp. such as L. innocua and L. welshimeri are more common than L. monocytogenes in food processing environments and may contribute to the dissemination of disinfectant resistance genes in listeriae, including L. monocytogenes. In this study, we investigated conjugative transfer of resistance to BC and to cadmium from nonpathogenic Listeria spp. to other nonpathogenic listeriae, as well as to L. monocytogenes. BC-resistant L. welshimeri and L. innocua harboring bcrABC, along with the cadmium resistance determinant cadA2, were able to transfer resistance to other nonpathogenic listeriae as well as to L. monocytogenes of diverse serotypes, including strains from the 2011 cantaloupe outbreak. Transfer among nonpathogenic Listeria spp. was noticeably higher at 25°C than at 37°C, whereas acquisition of resistance by L. monocytogenes was equally efficient at 25 and 37°C. When the nonpathogenic donors were resistant to both BC and cadmium, acquisition of cadmium resistance was an effective surrogate for transfer of resistance to BC, suggesting coselection between these resistance attributes. The results suggest that nonpathogenic Listeria spp. may behave as reservoirs for disinfectant and heavy metal resistance genes for other listeriae, including the pathogenic species L. monocytogenes.

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“…O patôgeno também foi detectado em raspados de tonsilas, em estudo realizado por Kanuganti et al (2002). Yokoyama et al (2005) Outros autores (FERRONATTO et al, 2012;SILVA et al, 2012;BARROS et al, 2007;WALSH et al, 1998) …”

Section: Resultsunclassified

PESQUISA E QUANTIFICAÇÃO DE Listeria sp. EM CARCAÇAS SUÍNAS ANTES E APÓS O PROCESSO DE RESFRIAMENTO EM CÂMARA FRIA

Martins¹,

Degenhardt²,

Thaler³

et al. 2014

AVS

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RESUMO:A Listeria monocytogenes tem sido reconhecido como um importante patógeno transmitido por alimentos, em decorrência de vários surtos de listeriose. Esta bactéria tem ainda a capacidade de sobreviver em condições adversas de temperatura e pH e a detecção de células estressadas em alimentos é importante, pois injúrias sub-letais às células podem provocar aumento de sua patogenicidade. O objetivo deste trabalho é determinar e quantificar a presença de Listeria monocytogenes em carcaças suínas antes e após a câmara fria. As coletas de amostras foram realizadas em um frigorífico localizado no Estado de Santa Catarina. Foram coletadas 226 amostras de carcaças. Foram utilizadas esponjas para a coleta das amostras em quatro pontos de 100 cm 2 nas carcaças, totalizando uma área de 400 cm 2 . Os pontos de coleta foram no pernil, lombo, barriga e papada. No laboratório, as amostras foram acrescidas de 100 mL de caldo de pré-enriquecimento UVM e homogeneizadas no caldo. O caldo foi incubado a 30°C/24h e posteriormente repicado 0,1 mL da cultura para o caldo Fraser, e incubado a 36ºC/24h. As amostras presuntivamente positivas foram repicadas para Ágar ALOA para isolamento (36°C/24h). As colônias suspeitas foram identificadas por meio de provas bioquímicas e CAMP-teste. Para a quantificação foi utilizada a técnica de Número Mais Provável em série de 10 tubos. Das amostras analisadas, 0,44% (1) SEARCH AND QUANTIFICATION OF LISTERIA SP. IN PIG CARCASSES BEFORE AND AFTER THE COOLING PROCESS IN COOL CHAMBERABSTRACT: Listeria monocytogenes has been recognized as an important pathogen transmitted by food, due to several outbreaks of listeriosis. This bacterium also has the ability to survive under adverse conditions of temperature and pH and the detection of stressed cells in foods is important because sub-lethal cell injury may lead to increased pathogenicity. The objective of this study is to determine and quantify the presence of Listeria monocytogenes in pig carcasses before and after the cool chamber. Material sampling was carried out at one processing plant located in the State of Santa Catarina. 226 samples were collected from carcasses. Sampling was performed by means of sponge and the smear sampling area which covered a defined area of 100 cm 2 per sampling, totaling an area of 400 cm 2 . The points were in the ham, loin, belly and jowl. At the laboratory, samples were added to 100 ml of pre-enrichment broth UVM (University of Vermont) and homogenized in the broth. The broth was incubated at 30°C/24h and subsequently peaked 0.1 ml of culture to Fraser broth and incubated at 36° C/24h. The samples presumptively positive were peaked for ALOA Agar for isolation (incubation at 36°C/24h). The suspected colonies were identified by biochemical tests and test-CAMP. For quantification we used the technique of Most Probable Number. From the samples analyzed within the establishments A, 0.44% (1/226) samples were positive for L. monocytogene. An increase of positive results for Listeria sp. was found on the pig carcases after...

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COMPARISON OF SELECTIVE AND NONSELECTIVE ENRICHMENT MEDIA FOR THE ISOLATION OF LISTERIA SPECIES FROM RETAIL FOODS

Cited by 22 publications

References 26 publications

Physical and metabolic causes of sub-lethal damage in Listeria monocytogenes after long-term chilled storage at 4 oC

Physical and metabolic causes of sub-lethal damage in Listeria monocytogenes after long-term chilled storage at 4 oC

Coselection of Cadmium and Benzalkonium Chloride Resistance in Conjugative Transfers from Nonpathogenic Listeria spp. to Other Listeriae

PESQUISA E QUANTIFICAÇÃO DE Listeria sp. EM CARCAÇAS SUÍNAS ANTES E APÓS O PROCESSO DE RESFRIAMENTO EM CÂMARA FRIA

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