Comparison of seed proteins of some representatives of the genusPisum from the point of view of their relationship comparison by disc electrophoresis

Hadačová, Věra; Turková, Věra; Hadač, Emil; Klozová, Eva

doi:10.1007/bf02878122

Cited by 13 publications

(4 citation statements)

References 11 publications

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“…Early hypothesis suggested that Ethiopian pea was simply a geographically isolated variety of common pea, but soon it became clear that it was a separate species domesticated in Ethiopia (Wetterstrom 2006). The results of diverse tests, including ecogeographical (Maxted & Ambrose 2001), genetic (Baranger et al 2004) and biochemical (Hadačová et al 1980) confirmed the view that Ethiopian pea deserved a status of a separate species within the genus Pisum L., together with red yellow (P. fulvum Sm.) and common peas.…”

Section: Ethiopian Pea (Pisum Abyssinicum a Braun)supporting

confidence: 55%

10.5937/ratpov51-5072 = Significance of genetic resources of cool season annual legumes: II: Neglected and underutilised crops

Mikić¹,

Mihailović²

2014

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Plant genetic resources are a live treasury of both one country and the whole mankind, although their ex situ preservation and in situ conservation are very demanding in numerous ways, especially if human resources and financial issues are considered. Legumes (Fabaceae Lindl.) are facing the bottlenecks caused by breeding emphasizing yield and quality, raising the questions how to solve the issue of this narrowing the genetic basis of cultivated legume species. The reintroduction of neglected and underutilised crops, such as red vetchling (Lathyrus cicera), Cyprus vetchling (Lathyrus ochrus), French serradella (Ornithopus sativus), Ethiopian pea (Pisum abyssinicum), field pea (Pisum sativum var. arvense), fenugreek (Trigonella phoenum-graecum), bitter vetch (Vicia ervilia), Narbonne vetch (Vicia narbonensis), Hungarian vetch (Vicia pannonica) and hairy vetch (Vicia villosa) may significantly assist many contemporary farming systems by diversifying their needs and improve the efficiency of environment resources.

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Section: Ethiopian Pea (Pisum Abyssinicum a Braun)supporting

confidence: 55%

10.5937/ratpov51-5072 = Significance of genetic resources of cool season annual legumes: II: Neglected and underutilised crops

Mikić¹,

Mihailović²

2014

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“…Quantitative interpretation was based on the intensity of the colouring of protein variants in the resulting electrophoretic spectrum. If heterogeneous spectra appeared, the identity index (ii) for the determination of related electrophoretic spectra was estimated (Hadačová et al 1980). These spectra were plotted using the Žížala programme in MS Excel by REM and the intensity of the colouring of protein variants.…”

Section: Methodsmentioning

confidence: 99%

Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker

Vyhnánek¹,

Bednář²

2003

Plant Soil Environ.

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In 1997 and 1998 we used samples of harvested grain to verify the possibility of distinguishing 14 winter wheat genotypes and six triticale genotypes and detecting the impurity on the basis of the detection of polymorphism of prolamin kernel proteins using the methods of the PAGE ISTA. On the basis of the identity index two sister prolamin lines with different percentage of participation, which was based on the weather conditions of the year of harvest, were discovered in seven wheat genotypes (Astella, Brea, Hana, Ilona, Siria, Sofia and Šárka) and two triticale genotypes (Tornádo and KM 779). A foreign genotype was detected in the Hana and Astella varieties. The identity index of the impurity to the Astella and Hana variety (i.e. ii = 0.28 and ii = 0.20, respectively) was considerably lower. In an unknown genotype (impurity) we detected the gliadin block Gld 1B3, which is the genetic marker of rye translocation T1BL.1RS, the Sr31 gene of resistance to black rust, higher cold resistance and the marker of poor baking quality (presence of secalin genes). The results proved the potential practical application of the method of electrophoretic detection of polymorphism of prolamin proteins as markers of impurities of foreign genotypes in a seed sample.Keywords: winter wheat; winter triticale; prolamin proteins; electrophoresis; admixture In breeding work it is important to make sure to use initial seed stock that has the required properties, but also to use material of corresponding varietal (genotypic) purity. This also applies to farmers who produce and sell seeds in the following year and, last but not least, it concerns the processing industry where admixtures of an undesirable genotype have a negative effect and result in poorer quality of the end product.Both wheat (Triticum aestivum L.) and triticale (Xtriticosecale Wittmack) are self-pollinating crops with a low share of cross-pollination (4-5%). This means that most of the varieties are of the line type or they are a mixture of isogenic lines (Chloupek 2000). A number of methods can be used to detect the impurities in a sample of harvested seeds. In our case we used the polymorphism of reserve kernel proteins, i.e. the alcohol-soluble fraction -prolamins (gliadins, secalins). High polymorphism was used by many authors (Šašek and Sýkorová 1989, Metakovsky 1991, Černý et al. 1992 and is characteristic of these proteins. Compared to other variability markers they offer a number of advantages.Compared to other biochemical markers, the isoenzymes, they are not so dependent on conditions of the external environment and they are independent on the ontogenetic stage of the plant. Other markers, which are undergoing considerable development at the present time, are monitoring on the level of DNA polymorphism. However, compared to the detection of polymorphism of prolamin proteins these methods are much more expensive due to the used chemicals and technology.In terms of the above aspects, polymorphism of reserve proteins is more suitable for the detection of ...

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“…Quantitative interpretation was based on the intensity of the colouring of protein bands in the resulting electrophoretic pattern (Černý and Šašek, 1996). If heterogeneous patterns appeared, the identity index (ii) for the determination of the relation of electrophoretic patterns was estimated (Hadačová et al, 1980). These patterns were plotted using MS Excel by REM and intensity of the colouring of protein bands.…”

Section: Varietymentioning

confidence: 99%

Polymorphism of prolamin proteins in selected varieties of winter wheat registered in the Czech Republic

Vyhnánek¹

2014

Acta Univ. Agric. Silvic. Mendelianae Brun.

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The need for vernalization of winter wheat varieties cultivated in the Czech Republic in 2000 was studied in comparison with the need for vernalization in the past decades since 1950. In 2000, many foreign varieties were cultivated in the Czech Republic, mostly West European. Varieties with a vernalization of 40-50 days and 50-60 days show the highest representation in the assortment (47.3% and 31.6%, resp.). The share of varieties with a long vernalization over 60 days is 15.8%. In around 1990, when varieties of domestic breeding were mostly grown, there were, next to the largest group with a vernalization of 40-50 days, 21.7% of varieties with a vernalization of 30-40 days and the same amount with a vernalization of 50-60 days. During the last ten years, the share of varieties with a longer vernalization has risen, not only due to foreign varieties, but also due to new domestic varieties. It is apparent from a 50-year overview that what has predominated are varieties with a vernalization of 40-50 or 40-60 days, which is a range usual for winter varieties of wheat in Middle and West Europe. After 1950, a departure from original domestic varieties appeared; those were represented by original alternative varieties (in Czech pøesívky, in German Wechselweizen, in Russian dvuruèki) and half-winter varieties with a shorter vernalization, strictly speaking with a vernalization fixed to a short day, and a strong photoperiodic reaction. Representation of varieties as related to their length of vernalization has changed in the course of the decades following utilization of foreign varieties; this was affected above all by varieties from Russia (the former USSR), Germany, but also Yugoslavia. Varieties from these countries were utilized also as parent components in domestic breeding.

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Comparison of seed proteins of some representatives of the genusPisum from the point of view of their relationship comparison by disc electrophoresis

Cited by 13 publications

References 11 publications

10.5937/ratpov51-5072 = Significance of genetic resources of cool season annual legumes: II: Neglected and underutilised crops

10.5937/ratpov51-5072 = Significance of genetic resources of cool season annual legumes: II: Neglected and underutilised crops

Detection of the varietal purity in sample of harvested wheat and triticale grains by prolamin marker

Polymorphism of prolamin proteins in selected varieties of winter wheat registered in the Czech Republic

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