Comparison of Sebia Capillarys Capillary Electrophoresis With the Primus High-Pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies

Abstract

The Sebia Capillarys (capillary zone electrophoresis [CE]) and the Primus Resolution high-pressure liquid chromatography (HPLC) were used to prospectively evaluate 297 samples for hemoglobinopathies. Hemoglobin (Hb) A levels were similar on both techniques (mean, 96.2% and SD, 5.7% by CE; mean, 96.8% and SD, 5.5% by HPLC), but HbA2 levels were higher by CE (mean, 2.8%; SD, 0.8%) than by HPLC (mean, 2.3%; SD, 0.8%). HbS had higher values by CE (mean, 40.6%; SD, 18.9%) than by HPLC (mean, 38.4%; SD, 18.9%). In cases with Hg S, HbA2 levels were greater by HPLC (mean, 4.0%; SD, 1.0%) than by CE (mean, 3.1%; SD, 0.8%). HbA2 was occasionally not separated sufficiently from HbC for measurement by CE, but did separate from HbE by CE. Both methods identified HbS, HbC, HbE, HbS, and HbC together, HbA2&prime, HbD-Los Angeles, HbF variant, HbG-Philadelphia, HbS-G Philadelphia, and Hb Lepore.

Comparison of capillary electrophoresis and high performance liquid chromatography for detection and quantification of hemoglobin New York

“…In this case, the quantification is not accurate. However, using CE, many variants can be detected easily and quantified accurately [6,7], including Hb New York, even if combined with Hb E, that can be also separated from Hb A [12].…”

“…Some studies showed that CE was superior to HPLC for detection of thalassemia and Hb variants [6,7], however the performance of these methods for the detection of Hb New York was not explored. The aim of this study is to compare CE and HPLC for detection and quantification of Hb New York.…”

Potential of the Sebia Capillarys® neonat fast automated system for neonatal screening of sickle cell disease

“…Minor and major HbBart's were also detected. This technique is also able to identify HbLepore (10). False positives found classically by IEF (2) are not seen with CE.…”

Variantes de hemoglobina en una población con impresión diagnóstica positiva para hemoglobinopatías en Colombia

“…En cuanto a comparación técnica, cuando se empleó EC en el caso particular de la baja correlación de Hb A2 y aumento de los valores de la misma, se evidenció un resultado similar al obtenido por Keren y cols 21 , compararon EC y HPLC para el diagnóstico de hemoglobinopatías, obteniendo ligero aumento de Hb A2 por el primer método.…”