Comparison of scpB gene and cfb gene polymerase chain reaction assays with culture on Islam medium to detect Group B Streptococcus in pregnancy

Shabayek, Sarah; Abdalla, Salah; Abouzeid, Abouzeid M H

doi:10.4103/0255-0857.71821

Cited by 9 publications

(8 citation statements)

References 21 publications

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“…Previous studies have reported that PCR done on aliquots from LIM broth was more sensitive than culture [9,[24][25][26][27]. In our study, 62 (12.4%) samples were positive by PCR from LIM broth.…”

Section: Discussionsupporting

confidence: 48%

Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey

Alp

Fındık

Dagi

et al. 2016

J Infect Dev Ctries

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Introduction: The purpose of this study was to investigate group B streptococcus (GBS) colonization, to compare the methods, to determine the relationship between GBS carriage and risk factors, and to genotype the GBS isolates. Methodology: Recto-vaginal swab specimens were obtained from 500 women, and a questionnaire was administered to each to assess their risk factors for GBS carriage. A culture, GBS antigen test, and polymerase chain reaction (PCR) were performed on all samples. Antibiotic susceptibility testing was performed, and the clonal relationship was determined by pulsed-field gel electrophoresis (PFGE) on all viable isolates. Results: Of the 500 women, sixty-eight (13.6%) women were GBS carriers, of whom 9.8% were pregnant and 16.5% not. There was a significant difference between GBS carriage and history of premature rupture of membrane (PROM). GBS was isolated from 65 (13%) samples. GBS was positive in 70 (14%) samples by antigen test and in 62 (12.4%) by PCR. Sixty-eight of the 70 positive antigen tests were confirmed by PCR or culture. Fifty-five isolates were resistant to tetracycline, 16 to erythromycin and clindamycin, and 13 to levofloxacin. Thirteen different pulsotypes and 17 sporadic strains were determined by PFGE. Conclusions: GBS carriage rate in non-pregnant women was higher than in pregnant women. The GBS antigen test was more sensitive than culture and PCR. GBS isolates did not originate from a single clone and contained sporadic strains. There was a significant difference between GBS carriage and history of PROM. Epidemiologic data obtained in this study will help future studies.

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Section: Discussionsupporting

confidence: 48%

Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey

Alp

Fındık

Dagi

et al. 2016

J Infect Dev Ctries

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“…Increased GBS isolation rates by PCR diagnostic tests over standard culture methods have been reported in other studies [10,17]. Although available nucleic acid amplification tests have demonstrated high sensitivity when performed on enriched samples, enrichment is not feasible in the intrapartum setting when results are needed quickly.…”

Section: Discussionmentioning

confidence: 93%

“…from the incubated Lim broth using 500µl of the broth. DNA was prepared from lysed bacterial cells by phenol/chloroform extraction procedure as described by Shabayek et al, [10].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Culture, Antigen Detection and Polymerase Chain Reaction for Detection of Vaginal Colonization of Group B Streptococcus (GBS) in Pregnant Women

Konikkara

Baliga

Shenoy

et al. 2014

JCDR

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Background and Objective: Group B Streptococcal infection is an important cause of neonatal morbidity and mortality. Early detection of perinatal vagino-rectal (VR) carriage of Group B Streptococcus (GBS) is important in the management of newborn infections. The objective of the study was to evaluate Culture, antigen detection and Polymerase chain reaction for detection of GBS in Pregnant women. Settings and Design:Observational descriptive study was done in a tertiary care hospital in Southern India. Materials and Methods:VR swabs were collected from 50 women at 35 to 37 weeks of gestation. Culture in a selective Lim enrichment broth with subsequent culture on 5% sheep blood agar, Conventional PCR assay and antigen detection method were performed.The performance of antigen detection and PCR methods were compared with culture.Statistical analysis: Statistical analysis was performed by ChiSquare test.Results: GBS cultures were positive for 16% of the specimen (8 out of 50). Considering culture as a gold standard, Sensitivity, Specificity, Positive predictive value and Negative predictive value of antigen detection was 100%, 92.86%, 72.73%, 100% and similarly for that of PCR was 100%, 45.23%, 25.80%, 100%, respectively. Conclusion:Antigen detection method was the rapid, sensitive and specific test for the detection of GBS colonizers during pregnancy.

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“…In the last decade, molecular techniques and in particular, polymerase chain reaction (PCR) tests have been developed for the rapid detection of GBS even after admission in the delivery room. Characterized by higher sensitivity and specificity, the rapid PCR assays have gained increased attention over the timeconsuming culture-based methods [5][6][7][8].…”

Section: Introductionmentioning

confidence: 99%

Antenatal group B streptococcus detection in pregnant women: culture or PCR?

Gerolymatos

Karlovasiti

Sianou

et al. 2018

J Infect Dev Ctries

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Introduction: Group B streptococcus (GBS) is an important cause of neonatal infections. Maternal GBS colonization screening and intrapartum antimicrobial prophylaxis of colonized women can prevent neonatal diseases. The aim of this study was to assess the prevalence of GBS colonization in pregnant and non-pregnant women and to compare the performance of a polymerase chain reaction (PCR) assay with the established as gold standard technique, culture method, used for the detection of this microorganism. Methodology: Vaginal and rectal samples collected from 857 pregnant and 370 non-pregnant women were examined through cultures, while the samples collected from 452 pregnant women between 35 and 37 weeks of gestation were assayed by culture and PCR method targeting the cfb gene. Results: GBS colonization was present in both pregnant and non-pregnant women. The colonization rate was similar in non-pregnant and first trimester pregnant women and then increased from first to the third trimester of pregnancy. GBS cultures for vaginal and rectal samples were positive in 13.2% and 14.3% in non-pregnant women, while in pregnant women 13.2% and 13.7% in the first trimester, and 15.0% and 16.5% in the second trimester, respectively. In third trimester pregnant women, compared to culture method, PCR identified a significantly increased number of GBS positive vaginal (18.4% vs 22.6%, p = 0.0006) and rectal (18.1% vs 21.2%, p = 0.01) samples. Conclusions: GBS colonization rate was higher in the third trimester. PCR proved to be a rapid and useful GBS screening method allowing a shorter detection time, while identifying more colonized women than culture.

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Comparison of scpB gene and cfb gene polymerase chain reaction assays with culture on Islam medium to detect Group B Streptococcus in pregnancy

Cited by 9 publications

References 21 publications

Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey

Screening and genotyping of group B streptococcus in pregnant and non-pregnant women in Turkey

Evaluation of Culture, Antigen Detection and Polymerase Chain Reaction for Detection of Vaginal Colonization of Group B Streptococcus (GBS) in Pregnant Women

Antenatal group B streptococcus detection in pregnant women: culture or PCR?

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