Comparison of Schistosoma Mansoni Migration Patterns in Normal and Irradiated Cercaria-Immunized Mice by Means of Autoradiographic Analysis

Dean, David A.; Mangold, Beverly L.; Georgi, Jay R.; Jacobson, Richard H.

doi:10.4269/ajtmh.1984.33.89

Cited by 82 publications

(49 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These parasite stages have been implicated as targets for protective immunity in murine vaccine models (16,17). Supernatants from cultures containing paramyosin-sensitized cells and either schistosome stage were found to contain significant levels of IFN-y as measured by an enzyme-linked immunoassay (Table 3).…”

Section: Resultsmentioning

confidence: 99%

Induction of protective immunity against Schistosoma mansoni by vaccination with schistosome paramyosin (Sm97), a nonsurface parasite antigen.

Pearce

James²,

Hieny³

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

192

107

View full text Add to dashboard Cite

Paramyosin (Sm97), a 97-kDa myofibrillar protein identified by the unusually monospecific antibody response induced by intradermal vaccination of mice with a complex soluble worm antigen preparation (SWAP) of adult Schistosoma mansoni administered with bacillus CalmetteGuirin (BCG), was purified and tested for its capacity to protect mice against challenge infection. When administered intradermally with BCG at total doses of only 4-40 jug per mouse, both the native molecule and a recombinant expression product containing approximately 50% of the whole protein were found to confer significant resistance (26-33%) against challenge infection, while 2 mg of unfractionated SWAP was required to induce similar levels of protection. In addition, paramyosin was shown to stimulate T lymphocytes from vaccinated mice to produce lymphokines [e.g., y interferon (IFN-y)] that activate macrophages to kill schistosomula.

show abstract

Section: Resultsmentioning

confidence: 99%

Induction of protective immunity against Schistosoma mansoni by vaccination with schistosome paramyosin (Sm97), a nonsurface parasite antigen.

Pearce

James²,

Hieny³

et al. 1988

Proc. Natl. Acad. Sci. U.S.A.

192

107

View full text Add to dashboard Cite

show abstract

“…Evidence exists that the lung is an important site of schistosomule elimination in resistant mice (16)(17)(18). To sensitize the lungs of mice, crude adult worm homogenates of either adult male or female schistosomes were administered i.n.…”

Section: Lack Of Resistance To Infection After Intranasal Administratmentioning

confidence: 99%

Attempts to induce resistance in mice to Schistosoma japonicum and Schistosoma mansoni by exposure to crude schistosome antigens plus cloned glutathione‐S‐transferases

Mitchell¹,

Davern²,

Wood³

et al. 1990

Immunology & Cell Biology

View full text Add to dashboard Cite

Summary Several attempts have been made to induce resistance in mice to Schistosoma japonicum (Philippines) or Schistosoma mansoni by exposure to living male and/or female adult worms, their antigens or irradiated cercariae. No resistance was demonstrated in the following cases: re-exposure of mice to cercariae following praziquantel (PZQ) treatment of existing infection; re-exposure of mice following cyclosporin A (CsA) treatment at the time of first cerearial exposure; subcutaneous or intraperitoneal deposition of living male or female worms; repeated intranasal administration of crude worm homogenates plus Bordetella pertussis vaccine (BPV) as adjuvant. Homologous ^^Co-irradiated cercariae were very effective at inducing resistance to infection with 5. mansoni but not to infection with S. japonicum (Philippines) in a limited series of experiments. A regime of infection, immunization with homologous Escherichia co//-derived glutathione-S-transferases (GST), then PZQ treatment followed by homologous re-exposure did not result in significant resistance in either the 5. mansoni or the S. japonicum (Philippines) systems. Mice given irradiated cercariae plus GST were not more resistant to subsequent 5. mansoni infection than mice given irradiated cercariae alone. The results generally confirm and extend those reported by others with the conclusion that resistance to schistosomes in mice is difficult to achieve by exposure to adult worm antigens alone. Moreover, additional immunization with the GST available to date as cloned gene products, and injected in Freund's complete adjuvant, does not influence the outcome of exposure to crude worm antigens including any additive effects of protective irradiated cercariae.

show abstract

“…The migration from the skin to the portal system involves a complex series of events in which the parasites spend an average of 2 to 5 days penetrating the skin before entering the blood vessels, where they are transported through the lungs before arriving in the hepatoportal system. Approximately 50 to 70% of cercariae never reach the hepatoportal system and are cleared in the lungs (15,16,49). While in the lung, the parasite is of such a size that it may completely occlude the vessels and will have to squeeze its way through the capillaries, distending them, and in some cases causing endothelial damage (13), and it is likely that the elasticity of the capillaries may be of importance in permitting the migration of schistosomula through the lungs.…”

Section: Discussionmentioning

confidence: 99%

“…In order to investigate the influence of T. muris infection on the cytokine environment in the lungs during early S. mansoni larval migration, we analyzed cytokine mRNA expression in lungs on day 7 post-S. mansoni infection by real-time PCR. This time point was chosen since it represents the peak for larval passage through the lungs (16,50). The data in Fig.…”

mentioning

confidence: 99%

Chronic Intestinal Nematode Infection Exacerbates ExperimentalSchistosoma mansoniInfection

2008

View full text Add to dashboard Cite

Mixed-parasite infections are common in many parts of the world, but little is known of the effects of concomitant parasite infections on the immune response or on disease progression. We have investigated the in vivo effects of a chronic gastrointestinal nematode infection on the infectivity and development of the immune response against the common trematode helminth Schistosoma mansoni. The data show that mice carrying an established chronic Trichuris muris infection and coinfected with S. mansoni, had significantly higher S. mansoni worm burdens than mice without coinfection. The increase in S. mansoni worm burden was accompanied by a higher egg burden in the liver. Kinetic analysis of S. mansoni establishment indicate reduced trapping of S. mansoni larvae during skin-to-lung migration, with T. muris-induced alterations in lung cytokine expression and inflammatory foci surrounding lung-stage schistosomula, suggesting that the immunomodulatory effects of chronic T. muris infection elicited at the gut mucosal surface extend to other organs and perhaps specifically to other mucosal surfaces. The data show that a preexisting chronic gastrointestinal nematode infection facilitates the survival and migration of S. mansoni schistosomula to the portal system, and as a result, increases the egg burden and associated pathology of S. mansoni infection.Helminth infections are among the most common infections of humans (2,7,26). Helminth infections are characteristically chronic in nature, and in order to achieve such long-lasting infections, these parasites have developed sophisticated survival strategies such as secretion of immunomodulatory substances and/or the induction of regulatory immune responses (35). These immunomodulatory activities may alter immune responsiveness to third party antigens such as vaccines (9, 10, 18, 42) and concurrent infections (reviewed in references 4 and 27). Intriguingly, recent studies have also demonstrated that the immunomodulatory effects of helminth infections may have beneficial effects, such as controlling allergy and inflammatory diseases (19,53).Individuals living in areas where helminths are endemic often carry more than one species of worm infection; egg output for an individual helminth species is often higher in individuals carrying mixed infections than in individuals carrying singlespecies infections (3,5,20,38,45), which may reflect higher intensities of infection and so a higher risk of morbidity. Geographical and environmental factors are known to be of significance in facilitating this type of polyparasitism: for example, similarities in the transmission pathways for certain soil-transmitted nematodes are likely to account for some of the observed associations. However, significant associations have also been demonstrated between helminth infections that do not share obvious transmission pathways and some of these associations are not explained solely by household or environmental effects (20). Furthermore, the intensity of helminth infections alters the risk of multipl...

show abstract

Comparison of Schistosoma Mansoni Migration Patterns in Normal and Irradiated Cercaria-Immunized Mice by Means of Autoradiographic Analysis

Cited by 82 publications

References 0 publications

Induction of protective immunity against Schistosoma mansoni by vaccination with schistosome paramyosin (Sm97), a nonsurface parasite antigen.

Induction of protective immunity against Schistosoma mansoni by vaccination with schistosome paramyosin (Sm97), a nonsurface parasite antigen.

Attempts to induce resistance in mice to Schistosoma japonicum and Schistosoma mansoni by exposure to crude schistosome antigens plus cloned glutathione‐S‐transferases

Chronic Intestinal Nematode Infection Exacerbates ExperimentalSchistosoma mansoniInfection

Contact Info

Product

Resources

About