“…[32][33][34][35][36] However, PCR requires strict experimental conditions and was prone to cross contamination, resulting in false negative and false positive results. 37 LAMP, SDA, and RCA could be used for constant temperature amplification without the need for special heating equipment. However, these methods rely on the use of enzymes, and the enzyme is expensive, have a short storage time, and the enzyme activity is easily affected by complex environmental conditions, which limit its further application.…”