Comparison of sampling bags for the analysis of volatile organic compounds in breath

Ghimenti, Silvia; Lomonaco, T; Bellagambi, Francesca G.; Tabucchi, S.; Onor, Massimo; Trivella, Maria Giovanna; Ceccarini, Alessio; Fuoco, Roger; Francesco, Fabio Di

doi:10.1088/1752-7155/9/4/047110

Cited by 66 publications

(76 citation statements)

References 34 publications

(54 reference statements)

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“…Hence, to determine the background contamination level on the m / z signals of interest, clean and flushed bags were filled with nitrogen and measured in scan mode by PTR‐MS after 0 and 72 h. On the first day, a few chemical compounds were detected in the blank bags at low concentration levels (<10 nL L −1 ), whereas for Tedlar, elevated levels of N,N‐dimethylacetamide (DMAC, m / z 88) and phenol ( m / z 95) were found. This corresponds well with the findings of previous studies (Pet'ka et al, 2000; Steeghs et al, 2007; Beauchamp et al, 2008; Ghimenti et al, 2015; Mochalski et al, 2013). During the 72‐h period, some m / z signals were found to increase, possibly due to off‐gassing from the polymer material or equilibration with room air due to diffusion.…”

Section: Methodssupporting

confidence: 93%

Mechanisms of Loss of Agricultural Odorous Compounds in Sample Bags of Nalophan, Tedlar, and PTFE

Kasper¹,

Oxbøl

Hansen³

et al. 2018

J of Env Quality

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Alteration of the chemical composition of odor samples during storage in polymer sample bags can significantly impair the accuracy of subsequent odor evaluations. To overcome or minimize this effect, the mechanisms determining compound loss must be more thoroughly understood. The present study examines the storage stability of a selection of key odorants from livestock production in polymer sample bags of Nalophan, Tedlar, and polytetrafluoroethylene (PTFE). The compounds included are acetic acid, butanoic acid, propanoic acid, 3-methylbutanoic acid, hydrogen sulfide, methanethiol, dimethyl sulfide, trimethylamine, and 4-methylphenol. The fate of the unrecovered compound fractions is clarified by means of thermal desorption and concentric double bags, allowing estimation of the magnitude of losses due to adsorption and diffusion, respectively. The degree of recovery was found to be PTFE > Tedlar > Nalophan, and smaller ratios of bag surface area to sample volume improved the recovery significantly. Furthermore, PTFE bags were found far superior for maintaining the original sample humidity and for storing 4-methylphenol. Analysis of sample humidity, partitioning coefficients, and thermal desorption suggested that the loss in PTFE bags was mainly controlled by adsorption, whereas for Nalophan and Tedlar, compound loss is a combined effect of adsorption and diffusion. It is suggested to heat the bags when evacuating the sample for analysis, as this was found to improve the recovery significantly. For a 5-L PTFE bag, all odorants could be found at concentration levels between 71.6 and 98.8% even after 48 h of storage when heated to 57°C prior to analysis.

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Section: Methodssupporting

confidence: 93%

Mechanisms of Loss of Agricultural Odorous Compounds in Sample Bags of Nalophan, Tedlar, and PTFE

Kasper¹,

Oxbøl

Hansen³

et al. 2018

J of Env Quality

View full text Add to dashboard Cite

show abstract

“…Analysis was performed within 20 minutes to avoid diffusive losses through the bag material, although previous studies have shown that such losses are not significant for wide-ranging compound types over a storage period of 24 hours. (see [16]). The room air was routinely analysed prior to coupling the sample bag to the SIFT-MS instrument.…”

Section: Breath Analysismentioning

confidence: 96%

Breath concentration of acetic acid vapour is elevated in patients with cystic fibrosis

Smith¹,

Sovová²,

Dryahina³

et al. 2016

J. Breath Res.

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A study has been carried out of the volatile organic compounds present in the exhaled breath of 58 cystic fibrosis (CF) patients. An important observation is that the acetic acid vapour concentration measured by selected ion flow tube mass spectrometry, SIFT-MS, is significantly elevated in the exhaled breath of CF patients, independent of the Pseudomonas aeruginosa (PA) infection status (PA-infected median 170 ppbv; PA-negative median 182 ppbv), compared to that of healthy controls (median 48 ppbv). The cause for this may be decreased pH of the mucus lining the CF airways. Thus, we speculate that non-invasive measurement of breath acetic acid concentration could serve as an indicator of the acidity of the CF airways mucosa.2

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“…Exhaled breath and breath condensate contain different volatile and nonvolatile fractions that provide complementary but different chemical information, and therefore each phase should be analyzed with specifically suitable methodology. In comparison to exhaled breath gas [30, 31], EBC contains more stable metabolomic content and allows easier sample manipulation, transfer, and storage [28]. …”

Section: Introductionmentioning

confidence: 99%

Human breath metabolomics using an optimized non-invasive exhaled breath condensate sampler

et al. 2016

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Exhaled breath condensate (EBC) analysis is a developing field with tremendous promise to advance personalized, non-invasive health diagnostics as new analytical instrumentation platforms and detection methods are developed. Multiple commercially-available and researcher-built experimental samplers are reported in the literature. However, there is very limited information available to determine an effective breath sampling approach, especially regarding the dependence of breath sample metabolomic content on the collection device design and sampling methodology. This lack of an optimal standard procedure results in a range of reported results that are sometimes contradictory. Here, we present a design of a portable human EBC sampler optimized for collection and preservation of the rich metabolomic content of breath. The performance of the engineered device is compared to two commercially available breath collection devices: the RTube™ and TurboDECCS. A number of design and performance parameters are considered, including: condenser temperature stability during sampling, collection efficiency, condenser material choice, and saliva contamination in the collected breath samples. The significance of the biological content of breath samples, collected with each device, is evaluated with a set of mass spectrometry methods and was the primary factor for evaluating device performance. The design includes an adjustable mass-size threshold for aerodynamic filtering of saliva droplets from the breath flow. Engineering an inexpensive device that allows efficient collection of metalomic-rich breath samples is intended to aid further advancement in the field of breath analysis for non-invasive health diagnostic. EBC sampling from human volunteers was performed under UC Davis IRB protocol 63701-3 (09/30/2014-07/07/2017).

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Comparison of sampling bags for the analysis of volatile organic compounds in breath

Cited by 66 publications

References 34 publications

Mechanisms of Loss of Agricultural Odorous Compounds in Sample Bags of Nalophan, Tedlar, and PTFE

Mechanisms of Loss of Agricultural Odorous Compounds in Sample Bags of Nalophan, Tedlar, and PTFE

Breath concentration of acetic acid vapour is elevated in patients with cystic fibrosis

Human breath metabolomics using an optimized non-invasive exhaled breath condensate sampler

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