1976
DOI: 10.1042/bj1580243
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Comparison of renal and hepatic glutathione S-transferases in the rat

Abstract: Renal and hepatic GSH (reduced glutathione) S-transferase were compared with respect to substrate and inhibitory kinetics and hormonal influences in vivo. An example of each of five classes of substrates (aryl, aralkyl, epoxide, alkyl and alkene) was used. In the gel filtration of renal or hepatic cytosol, an identical elution volume was found for all the transferase activities. Close correspondence in Km values was found for aryl, epoxide- and alkyl-transferase activities, with only the aralkyl activity signi… Show more

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Cited by 37 publications
(13 citation statements)
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“…GSH S-transferase activity was determined with 1-chloro-2,4-dinitrobenzene as the substrate (Habig et al, 1974). The enzyme assays with other substrates such as 4-nitropyridine N-oxide, 1,2epoxy-3-(p-nitrophenoxy)propane and p-nitrobenzyl chloride were performed by the method described elsewhere (Fjellstedt et al, 1973;Habig et al, 1974), and bilirubin and probenecid binding to the enzyme protein was done by the method of Ketley et al (1975) and Kaplowitz et al (1976). The unit of enzyme activity was defined as lumol of substrate conjugated or cleaved/min at 250C for GSH S-transferase and at 370C for GSH peroxidase.…”
Section: Materials and Methods Materialsmentioning
confidence: 99%
“…GSH S-transferase activity was determined with 1-chloro-2,4-dinitrobenzene as the substrate (Habig et al, 1974). The enzyme assays with other substrates such as 4-nitropyridine N-oxide, 1,2epoxy-3-(p-nitrophenoxy)propane and p-nitrobenzyl chloride were performed by the method described elsewhere (Fjellstedt et al, 1973;Habig et al, 1974), and bilirubin and probenecid binding to the enzyme protein was done by the method of Ketley et al (1975) and Kaplowitz et al (1976). The unit of enzyme activity was defined as lumol of substrate conjugated or cleaved/min at 250C for GSH S-transferase and at 370C for GSH peroxidase.…”
Section: Materials and Methods Materialsmentioning
confidence: 99%
“…It should be mentioned that several other GST inhibitors have been discovered belonging to different structural classes, including diethyl maleate, p ‐aminohippurate (Figure ) and probenecid, clofibrate, misonidazole, piriprost (Figure ), GSH conjugates of tetrachloro‐1,4‐benzoquinone derivatives, gossypol, and sulfasalazine (Figure ) …”
Section: Intrinsic Substrates and Metabolism‐related Drugsmentioning
confidence: 99%
“…Enzyme DIII,, and DIV,, seem to be more sensitive and show linear competitive kinetics as previously observed for rat [50] and human [46] liver transferases.…”
mentioning
confidence: 53%