Comparison of remote ischemic preconditioning and intermittent hypoxia training in fracture healing

Qiao, Junjie; Zhou, Meng; Zheng, Lirong; Ren, Jie; Gao, Guanghan; Cao, Guanglei; Shen, Hui-Liang; Lu, Shibao

doi:10.3892/mmr.2018.9788

Cited by 4 publications

(3 citation statements)

References 25 publications

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“…Hyperhomocysteinemia inhibits tibial fracture healing in rats through PI3K/AKT signaling pathway ( 44 ). In addition, Qiao et al ( 45 ) report that intermittent hypoxia training and remote ischemic preconditioning significantly enhance fracture healing; however, intermittent hypoxia training exhibited superior bone formation and healing effects compared with remote ischemic preconditioning, suggesting that the interaction of multiple signal pathways and various physiological conditions in the human body have an impact on the condition of the fracture.…”

Section: Discussionmentioning

confidence: 99%

Relationship between miRNA‑433 and SPP1 in the presence of fracture and traumatic brain injury

et al. 2021

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Limb fracture combined with traumatic brain injury (TBI) is one of the most common multiple injuries and patients often suffer from severe craniocerebral injury combined with long bone fracture of the limbs. The present study examined the expression of osteopontin (SPP1) in the tibial fracture callus and heterotopic ossification tissues in craniocerebral injury and investigated its relationship with miR-433. A total of 26 patients with tibial fracture combined with brain injury were included in the TBI group, and 26 patients with simple tibial fracture were included in the control group. The patients received immobilization treatment and callus was collected during the operation. At the time of steel plate removal tissue ossification samples from patients with heterotopic ossification were collected. Peripheral blood was collected from all patients on the morning of the operation day. Expression of miR-433 and SPP1 mRNA was determined by reverse transcription-quantitative PCR and SPP1 protein expression was measured by western blotting. Dual luciferase reporter assay was used to identify the direct interaction between miR-433 and SPP1 mRNA. The human osteoblast line hFOB1.19 was transfected with agomiR-433 to overexpress miR-433 and expression of SPP1 was also examined. TBI enhanced the incidence of callus formation and heterotopic ossification in patients with fracture but did not alter fracture healing time. SPP1 mRNA and protein expression was elevated in patients who had tibial fracture in combination with craniocerebral injury in comparison with controls By contrast, expression of miR-433 was decreased in patients who had tibial fracture in combination with craniocerebral injury in comparison with controls. miR-433 regulated the expression of SPP1 mRNA and protein by directly binding to the 3'-untranslated region of SPP1 mRNA. The present study suggests that SPP1 mRNA and protein levels are increased in the callus, heterotopic ossification tissues and plasma from patients with tibial fracture combined with brain injury in comparison with controls. This elevation may be due to the reduced expression of miR-433.

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Section: Discussionmentioning

confidence: 99%

Relationship between miRNA‑433 and SPP1 in the presence of fracture and traumatic brain injury

et al. 2021

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“… 52 Various literature has conducted studies on the age of mice and compared it with adult human ages. 70 The oldest age included in this review is the study by Qiao et al, 43 which used 72 to 80 weeks aged rats for human model of the elderly. In older rats, it was also reported that there was a decrease in their locomotor activity and limited tissue repair and regenerative capacity, which may affect the fracture healing process.…”

Section: Discussionmentioning

confidence: 99%

Temporal Pattern of micro-CT Angiography Vascular Parameters and VEGF mRNA Expression in Fracture Healing: a Radiograph and Molecular Comparison

et al. 2023

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Angiogenesis plays an important role in fracture healing with vascular endothelial growth factor (VEGF) as the main protein involved. Micro-computed tomography (CT) angiography may be used to analyze this revascularization with several parameters such as number of branches, total volume, and diameter. This systematic review is aimed to assess available studies on the temporal pattern of vascular imaging on micro-CT angiographs, especially in terms of the number of branches, total volume, and diameter as well as the temporal pattern of VEGF mRNA expression as the molecular comparison during bone fracture healing. This review was conducted according to the PRISMA guidelines. Electronic database searches were performed using PubMed, ProQuest, ScienceDirect, EBSCOhost, Taylor & Francis Online, and hand searching. The search strategy and keywords were adjusted to each database using the Boolean operators and other available limit functions to identify most relevant articles based on our inclusion and exclusion criteria. Screening and filtration were done in several stages by removing the duplicates and analyzing each title, abstract, and full-text in all included entries. Data extraction was done for syntheses to summarize the temporal pattern of each parameter. A total of 28 articles were eligible and met all criteria, 11 articles were synthesized in its angiograph's analysis, 16 articles were synthesized in its VEGF mRNA expression analysis, and 1 article had both parameters analyzed. The overall temporal pattern of both three micro-CT angiographic parameters and VEGF mRNA expression was in line qualitatively. The number of branches, total volume, and diameter of the blood vessels in micro-CT angiography showed an exponential rise at week 2 and decline at week 3 of fracture healing, with the VEGF mRNA expression concurrently showing a consistent pattern in the phase.

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“…Elevated levels of NO in response to VEGF binding to VEGF receptor type 2 (VEGFR2), can induce angiogenesis and regulate the endothelial function and migration ( 76 ). Some studies have identified that VEGF mRNA and protein expression level is up-regulated following RIC treatment ( 77 , 78 ). In a mouse model of spinal cord ischemia, ischemic preconditioning applied to the abdominal aorta (3 × 5 min of alternative clamping and reperfusion) resulted in high VEGF protein levels in plasma, with a resultant neuroprotective effect ( 78 ).…”

Section: Ric: Collateral Blood Flow Enhancementmentioning

confidence: 99%

Immune Modulation as a Key Mechanism for the Protective Effects of Remote Ischemic Conditioning After Stroke

2021

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Remote ischemic conditioning (RIC), which involves a series of short cycles of ischemia in an organ remote to the brain (typically the limbs), has been shown to protect the ischemic penumbra after stroke and reduce ischemia/reperfusion (IR) injury. Although the exact mechanism by which this protective signal is transferred from the remote site to the brain remains unclear, preclinical studies suggest that the mechanisms of RIC involve a combination of circulating humoral factors and neuronal signals. An improved understanding of these mechanisms will facilitate translation to more effective treatment strategies in clinical settings. In this review, we will discuss potential protective mechanisms in the brain and cerebral vasculature associated with RIC. We will discuss a putative role of the immune system and circulating mediators of inflammation in these protective processes, including the expression of pro-and anti-inflammatory genes in peripheral immune cells that may influence the outcome. We will also review the potential role of extracellular vesicles (EVs), biological vectors capable of delivering cell-specific cargo such as proteins and miRNAs to cells, in modulating the protective effects of RIC in the brain and vasculature.

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Comparison of remote ischemic preconditioning and intermittent hypoxia training in fracture healing

Cited by 4 publications

References 25 publications

Relationship between miRNA‑433 and SPP1 in the presence of fracture and traumatic brain injury

Relationship between miRNA‑433 and SPP1 in the presence of fracture and traumatic brain injury

Temporal Pattern of micro-CT Angiography Vascular Parameters and VEGF mRNA Expression in Fracture Healing: a Radiograph and Molecular Comparison

Immune Modulation as a Key Mechanism for the Protective Effects of Remote Ischemic Conditioning After Stroke

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