Comparison of refractometry and biuret assay for measurement of total protein concentration in canine abdominal and pleural fluid specimens

Rosé, Alexandra; Funk, Deborah; Neiger, Reto

doi:10.2460/javma.248.7.789

Cited by 10 publications

(6 citation statements)

References 16 publications

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“…In the current study, it was elected to use refractometry to estimate TPP, specifically because it can be performed bedside and is widely available. However, refractometry has limitations, mainly if interfering substances are present (e.g., lipid, hyperglycemia, synthetic colloids), as well as user error and variation 23 . Other clinically applicable and associated parameters, including hemoglobin and hematocrit, were not available for all cases and therefore were not included in this research.…”

Section: Discussionmentioning

confidence: 99%

Retrospective evaluation of admission total plasma protein as a predictor of red blood cell transfusion requirement in dogs diagnosed with traumatic and nontraumatic hemoabdomen: 90 dogs (2009–2019)

Buseman,

Blong,

Yuan

et al. 2023

J Vet Emergen Crit Care

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ObjectiveTo evaluate the association of admission total plasma protein (TPP) and the administration of red blood cell transfusions in dogs with diagnosed hemoabdomen. To secondarily evaluate additional point‐of‐care parameters associated with red blood cell transfusion administration.DesignRetrospective study between 2009 and 2019.SettingUniversity veterinary teaching hospital.AnimalsNinety dogs admitted to a university veterinary teaching hospital after a diagnosis of traumatic or nontraumatic hemoabdomen (NTH).Measurements and Main ResultsMedical records were retrospectively reviewed; signalment, point‐of‐care diagnostics, and transfusion administration information was recorded. A total of 47 dogs (traumatic hemoabdomen 11/26; NTH 36/64) received packed red blood cell transfusions. For each 1 g/dL unit decrease in TPP, dogs had an increased odds ratio (OR) of 2.14 (95% confidence interval [CI]: 1.44–3.40, P < 0.001) of receiving a red blood cell transfusion. Dogs diagnosed with NTH were more likely to receive a red blood cell transfusion than dogs with a traumatic hemoabdomen (OR: 2.78, 95% CI: 1.11–7.141, P = 0.03). Lower PCV values (OR: 1.08, 95% CI: 1.04–1.12, P < 0.001), bicarbonate values (OR: 1.3, 95% CI: 1.09–1.56, P = 0.003), and base excess (OR: 1.27, 95% CI: 1.1–1.49, P = 0.003) were associated with a higher likelihood of red blood cell transfusion. Additionally, higher lactate (OR: 1.35, 95% CI: 1.16–1.63, P < 0.001) and Acute Patient Physiologic and Laboratory Evaluation (APPLE)fast scores (OR: 1.10, 95% CI: 1.04–1.17, P < 0.001) were associated with increased red blood cell transfusion administration.ConclusionsLow admission TPP, independent of low PCV, was associated with red blood cell transfusions regardless of underlying cause. For each 1 g/dL unit decrease in TPP on presentation, dogs were approximately 2 times more likely to receive a red blood cell transfusion during hospitalization. Other factors that were associated with increased transfusion administration included presenting PCV, PCV/TPP ratio, bicarbonate, base excess, lactate, and APPLEfast scores.

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Section: Discussionmentioning

confidence: 99%

Retrospective evaluation of admission total plasma protein as a predictor of red blood cell transfusion requirement in dogs diagnosed with traumatic and nontraumatic hemoabdomen: 90 dogs (2009–2019)

Buseman,

Blong,

Yuan

et al. 2023

J Vet Emergen Crit Care

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“…We used the biuret reaction method to determine the amount of protein in solution. This method was chosen because of its simplicity and relative accuracy [14,15].…”

Section: Evaluation Of Plasma Protein Adsorption By Biocompositesmentioning

confidence: 99%

Obtaining New Biocompatible Composite Materials with Antibacterial Properties Based on Diatomite and Biologically Active Compounds

Kabieva,

Zhumanazarova,

Zhaslan

et al. 2024

Molecules

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This study aimed to create new composite materials based on diatomite—a non-organic porous compound—through its surface modification with bioactive organic compounds, both synthetic and natural. Chloramphenicol, tetrahydroxymethylglycoluril and betulin were used as modifying substances. Composite materials were obtained by covering the diatomite surface with bioactive substance compounds as a solution and material dispersion in it. The materials were characterized by IR spectroscopy, SEM and X-ray photoelectron spectroscopy. For the biocomposites, the hemolytic effect, plasma proteins’ adsorption on the surface and the antibacterial activity of the obtained materials were studied. Results show that the obtained materials are promising for medicine and agriculture.

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“…for TP measurement in canine pleural and abdominal fluid samples, providing a quicker, convenient and relatively inexpensive method, compared to the biuret biochemical assay (Rose et al, 2016).…”

Section: B Tncc and Tp Measurementmentioning

confidence: 99%

The laboratory diagnostic approach to thoracic and abdominal effusions in the dog, cat, and horse

Athanasiou

Spyropoulou

Meichner

2019

J Hellenic Vet Med Soc

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Cases involving pleural and peritoneal effusions occur relatively frequently in clinical practice. Determining the underlying etiology in these cases relies mainly on fluid analysis. The technique used for obtaining the pleural or peritoneal fluid can impact greatly the results of the analysis. Most often used diagnostic tools include evaluation of gross appearance, Total Nucleated Cell Count / Total Protein (TNCC/TP) measurement, chemical/biochemical analysis (Lactate dehydrogenase and lactate, cholesterol, triglycerides, glucose, creatinine, pH, pO2, pCO2, and K measurements), cytology (identification of septic and non-septic inflammation and neoplasia), microbiology (Gram stain, culture, molecular techniques), and specific tests for certain clinical conditions and diseases. Classifying an effusion as transudate, modified transudate and exudate is traditionally based on the TNCC and TP values. New diagnostic methods encourage the clinician to approach the effusion etiologically instead of strictly following this traditional classification. Many of the diagnostic tests described in this review are simple and can be performed in-house, providing the clinician quickly with information about the cause of the effusion, essential for an effective treatment plan without wasting valuable time.

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Comparison of refractometry and biuret assay for measurement of total protein concentration in canine abdominal and pleural fluid specimens

Abstract: Refractometry yielded acceptable results for measurement of TP concentration in canine pleural and abdominal fluid specimens, providing a more rapid and convenient method than biuret assay.

Cited by 10 publications

References 16 publications

Retrospective evaluation of admission total plasma protein as a predictor of red blood cell transfusion requirement in dogs diagnosed with traumatic and nontraumatic hemoabdomen: 90 dogs (2009–2019)

Retrospective evaluation of admission total plasma protein as a predictor of red blood cell transfusion requirement in dogs diagnosed with traumatic and nontraumatic hemoabdomen: 90 dogs (2009–2019)

Obtaining New Biocompatible Composite Materials with Antibacterial Properties Based on Diatomite and Biologically Active Compounds

The laboratory diagnostic approach to thoracic and abdominal effusions in the dog, cat, and horse

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