Comparison of red wine extract and polyphenol constituents on endothelin-1 synthesis by cultured endothelial cells

Khan, Noorafza Q.; Lees, Delphine M.; Douthwaite, Julie A.; Carrier, Martin; Corder, Roger

doi:10.1042/cs103s072s

Cited by 56 publications

(44 citation statements)

References 16 publications

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“…35,36 Resveratrol is a major component of the polyphenols from grapes and red wine. A number of studies have demonstrated the antioxidant effects of resveratrol.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Cyclic Strain-Induced Endothelin-1 Gene Expression by Resveratrol

et al. 2003

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Abstract-Resveratrol is a phytoestrogen naturally found in grapes and is among the major constituents of wine thought to have a cardioprotective effect. Endothelin-1 (ET-1) is a potent vasopressor synthesized by endothelial cells both in culture and in vivo. The aims of this study were to test the hypothesis that resveratrol may alter strain-induced ET-1 gene expression and to identify the putative underlying signaling pathways in endothelial cells. We show that resveratrol indeed potently inhibits strain-induced ET-1 secretion, ET-1 mRNA level, and ET-1 promoter activity. Resveratrol also inhibits strain-increased NADPH oxidase activity, reactive oxygen species formation, and extracellular signal-regulated kinases1/2 (ERK1/2) phosphorylation. Furthermore, pretreating cells with resveratrol or antioxidant N-acetyl-cysteine decreases strain-increased or hydrogen peroxide-increased ET-1 secretion, ET-1 promoter activity, and ET-1 mRNA and ERK1/2 phosphorylation. Using both the electrophoretic mobility shift assay and a reporter gene assay, resveratrol and N-acetyl-cysteine also attenuated the strain-stimulated activator protein-1 binding activity and activator protein-1 reporter activity. In summary, we demonstrate for the first time that resveratrol inhibits strain-induced ET-1 gene expression, partially by interfering with the ERK1/2 pathway through attenuation of reactive oxygen species formation. Thus, this study provides important new insights in the molecular pathways that may contribute to the proposed beneficial effects of resveratrol in the cardiovascular system. any epidemiological studies show a correlation between a low incidence of coronary heart disease and atherosclerosis and a moderate consumption of red wine. 1,2 The vasoprotective effect of red wine, also known as the "French paradox," is currently best exemplified by transresveratrol (trans-3,5,4Ј-hydroxystilbene). 3,4 Resveratrol has many biological activities, including protection from or reduction of the incidence of coronary heart disease. Resveratrol also has been found to protect the heart from ischemiareperfusion injury. 5 Antioxidant properties of resveratrol appear to be partly responsible for this activity. 5-9 Moreover, resveratrol was shown to relax aortic rings in rats through an endothelium-mediated enhancement of the nitric oxide (NO)-cGMP cascade. 10 Subsequent pharmacological studies indicate a direct relaxant effect of resveratrol on vascular smooth muscle that may exert beneficial effects in cardiovascular disease, though the mechanism of these effects is no known. 11 Recently, Orallo et al 12 demonstrated that the characteristic endothelium-dependent vasorelaxant effect of resveratrol in the rat aorta appeared to be caused by the inhibition of vascular NADH/NADPH oxidase and the subsequent decrease in the generation of basal cellular superoxide anions and, therefore, of NO biotransformation. However, little is known about the cellular/molecular mechanisms whereby resveratrol could protect against coronary heart disease. Indee...

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“…35,36 Resveratrol is a major component of the polyphenols from grapes and red wine. A number of studies have demonstrated the antioxidant effects of resveratrol.…”

Section: Discussionmentioning

confidence: 99%

Inhibition of Cyclic Strain-Induced Endothelin-1 Gene Expression by Resveratrol

et al. 2003

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“…This highly potent vasoconstrictor peptide, when elevated, is a strong predictor of bad outcome in chronic HF. 24,36 Red wine extracts have been shown to inhibit endothelin-1 synthesis by bovine aortic endothelial cells, 37 presumably by altering tyrosine-kinase signaling. 38 Polyphenols from red wine also blunt the expression of adhesion molecules and inflammatory cytokines related to endothelial dysfunction and atherosclerosis, contributing to a reduction in HF severity.…”

Section: Wine Consumption and Circulating Biomarkersmentioning

confidence: 99%

Regular Wine Consumption in Chronic Heart Failure

Cosmi

Giulio

Masson

et al. 2015

Circ: Heart Failure

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BACKGROUND:\ud Moderate, regular alcohol consumption is generally associated with a lower risk of cardiovascular events but data in patients with chronic heart failure are scarce. We evaluated the relations between wine consumption, health status, circulating biomarkers, and clinical outcomes in a large Italian population of patients with chronic heart failure enrolled in a multicenter clinical trial.\ud METHODS AND RESULTS:\ud A brief questionnaire on dietary habits was administered at baseline to 6973 patients enrolled in the Gruppo Italiano per lo Studio della Sopravvivenza nell'Insufficienza Cardiaca-Heart Failure (GISSI-HF) trial. The relations between wine consumption, fatal and nonfatal clinical end points, quality of life, symptoms of depression, and circulating biomarkers of cardiac function and inflammation (in subsets of patients) were evaluated with simple and multivariable-adjusted statistical models. Almost 56% of the patients reported drinking at least 1 glass of wine per day. After adjustment, clinical outcomes were not significantly different in the predefined 4 groups of wine consumption. However, patients with more frequent wine consumption had a significantly better perception of health status (Kansas City Cardiomyopathy Questionnaire score, adjusted P<0.0001), less frequent symptoms of depression (Geriatric Depression Scale, adjusted P=0.01), and lower plasma levels of biomarkers of vascular inflammation (osteoprotegerin and C-terminal proendothelin-1, adjusted P<0.0001, and pentraxin-3, P=0.01) after adjusting for possible confounders.\ud CONCLUSIONS:\ud We show for the first time in a large cohort of patients with chronic heart failure that moderate wine consumption is associated with a better perceived and objective health status, lower prevalence of depression, and less vascular inflammation, but does not translate into more favorable clinical 4-year outcomes

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“…Furthermore, improvements in inflammatory markers such as C-reactive protein and inhibition of the vasoactive peptide, endothelin À1 have been associated with improvements arterial stiffness (McEniery et al, 2003;Kampus et al, 2004;Pirro et al, 2004). Studies have shown that RW and RW polyphenols significantly reduce serum levels of C-reactive protein and strongly inhibits endothelin-1 synthesis, which may explain improvements in arterial stiffness (Corder et al, 2001;Khan et al, 2002;Estruch et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

The effect of chronic consumption of red wine polyphenols on vascular function in postmenopausal women

et al. 2006

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Objective: To elucidate whether the chronic consumption of dealcoholised red wine (DRW) (polyphenol-rich component) and/ or red wine (RW) improves vascular function in hypercholesterolaemic postmenopausal women. Design, subjects and intervention: A randomised parallel-arm study. Forty-five hypercholesterolaemic postmenopausal women were randomised into either water, DRW or RW group for 6 weeks following a 4 week washout. Fasting measures of central haemodynamic parameters, arterial wave reflection and endothelial nitric oxide were taken at 0 and 6 weeks. Setting: Clinic in the School of Public Health, Curtin University. Results: There were no significant between group differences in arterial stiffness as measured by augmentation index (AIx) and augmentation pressure (AP). However, a significant within group decrease in AIx (À9%, P ¼ 0.02) and AP (À12%, P ¼ 0.02) was observed following DRW consumption. No significant changes were observed in central haemodynamic parameters and endothelial nitric oxide levels following DRW and RW consumption, compared to water. Conclusions: Neither the chronic consumption of DRW nor RW improved markers of arterial stiffness, compared to control. However, the significant within group improvements in these indices following the consumption of DRW cannot be overlooked and warrant further investigation.

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Comparison of red wine extract and polyphenol constituents on endothelin-1 synthesis by cultured endothelial cells

Cited by 56 publications

References 16 publications

Inhibition of Cyclic Strain-Induced Endothelin-1 Gene Expression by Resveratrol

Inhibition of Cyclic Strain-Induced Endothelin-1 Gene Expression by Resveratrol

Regular Wine Consumption in Chronic Heart Failure

The effect of chronic consumption of red wine polyphenols on vascular function in postmenopausal women

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