Comparison of recombinant timothy grass pollen allergens with natural extract for diagnosis of grass pollen allergy in different populations☆, ☆☆, ★

Laffer, Sylvia; Spitzauer, Susanne; Susani, Markus; PAIRLEITNERA, H; Schweiger, Christian; GRONLUNDD, H; Menz, Günter; Pauli, G.; Ishii, Tetsuya; Nolte, Hendrik

doi:10.1016/s0091-6749(96)70099-4

Cited by 87 publications

(78 citation statements)

References 23 publications

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“…In spite of the important function, the structural variability of Zea m 1 between timothy and maize is high [31,11], which is also the case among Zea m 1 isoforms. In grasses, the IgE prevalence of 95% as determined in the case of Phl p 1 indicates that this protein represents the single most important grass pollen allergen [32]. The group 13 allergens were identified to function as polygalacturonases [33,34].…”

Section: Discussionmentioning

confidence: 99%

Proteome analysis of maize pollen for allergy-relevant components

et al. 2006

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Over the last few decades, the cultivation of maize (Zea mays) has strongly increased in Central Europe. We therefore decided to study the allergen composition and the allergenic potency of its pollen in comparison with pollen from timothy grass (Phleum pratense), a typical representative of the native grasses. We found that 65% of the sera reactive to timothy pollen also bound to maize pollen proteins. By using 2-DE immunoblotting, followed by incubation with mAbs directed against known allergens or protein sequencing, those IgE-reactive components were further classified. Although novel, maize-specific pollen allergens could not be found, the presence of crossreacting allergens belonging to groups 1 and 13 (Zea m 1 and 13), both having high IgE prevalence, as well as the presence of the less important group 3 and 12 allergens was found. The structural variability of Zea m 1 and Zea m 13 was determined by sequencing clones isolated from a maize pollen cDNA library. This revealed sequence identities of 72 and 70%, respectively, to the corresponding Phl p 1 and Phl p 13 allergens of timothy grass pollen. IgE-crossreactivity was further studied using immunoblot inhibition tests. Here, timothy pollen extract completely blocked IgE binding to maize, whereas maize pollen extract blocked IgE reactivity to only some timothy pollen allergens.

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Section: Discussionmentioning

confidence: 99%

Proteome analysis of maize pollen for allergy-relevant components

et al. 2006

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“…Skin tests with recombinant allergens were available in the 1990s for pollens (1199), molds such as Aspergillus (1200), mites (1201,1202), venoms (1203,1204) or latex (1205). Skin tests with recombinant and natural allergens have a similar value (1206)(1207)(1208)(1209)(1210) if the recombinant allergens have been well selected and represent all or most epitopes of the natural allergen (1211)(1212)(1213)(1214). Panels of recombinant allergens are available for the component-resolved diagnosis of allergy (1215).…”

Section: Skin Testsmentioning

confidence: 99%

“…Recombinant allergens have been used for the in vitro diagnosis of grass (1206,1207,1213,1246,1247), birch and Fagaleae (1248)(1249)(1250)(1251)(1252)(1253), Oleaceae (1199,1254), pollens or mites (1255)(1256)(1257). A single recombinant allergen or a combination of a few major recombinant allergens can substitute the crude extract for in vitro diagnostic purposes (1258,1259).…”

Section: In Vitro Testsmentioning

confidence: 99%

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Bousquet¹,

Khaltaev²,

Cruz³

et al. 2008

Allergy

3,749

1,753

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“…The group 5 allergen in the Poaceae subfamily is a major allergen [20,24], and to date is the only allergen that has been quantified in immunotherapy extracts. However, no authors have described group 5 allergens in C dactylon to date.…”

Section: Discussionmentioning

confidence: 99%

Relevance of Allergenic Sensitization to Cynodon dactylon and Phragmites communis: Cross-reactivity With Pooideae Grasses

Ma¹,

Moya²,

Cardona³

et al. 2016

J Investig Allergol Clin Immunol

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Background and Objectives: The homologous group of sweet grasses belongs to the Pooideae subfamily, but grass pollen species from other subfamilies can also cause allergy, such as Cynodon dactylon (Chloridoideae) and Phragmites communis (Arundinoideae). C dactylon and P communis have not been included in the sweet grasses homologous group because of their low cross-reactivity with other grasses. The aims of this study were to investigate the profile of sensitization to C dactylon and P communis in patients sensitized to grasses and to analyze cross-reactivity between these 2 species and temperate grasses. Methods: Patients were skin prick tested with a grass mixture (GM). Specific IgE to GM, C dactylon, P communis, Cyn d 1, and Phl p 1 was measured by ImmunoCAP. A pool of sera was used for the immunoblot assays. Cross-reactivity was studied by ELISA and immunoblot inhibition. Results: Thirty patients had sIgE to GM. Twenty-four (80%) had positive results for C dactylon, 27 (90%) for P communis, 22 (73.3%) for nCyn d 1, and 92.9% for rPhl p 1. Bands were detected in the 3 extracts by immunoblot. Inhibition of GM was not observed with C dactylon or P communis by immunoblot or ELISA inhibition. When C dactylon or P communis were used in the solid phase, GM produced almost complete inhibition. Conclusions: Eighty percent of patients sensitized to grasses were also sensitized to C dactylon and 90% were sensitized to P communis. Sensitization to these species seems to be induced by allergens different to those in sweet grasses.

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Comparison of recombinant timothy grass pollen allergens with natural extract for diagnosis of grass pollen allergy in different populations☆, ☆☆, ★

Cited by 87 publications

References 23 publications

Proteome analysis of maize pollen for allergy-relevant components

Proteome analysis of maize pollen for allergy-relevant components

Allergic Rhinitis and its Impact on Asthma (ARIA) 2008*

Relevance of Allergenic Sensitization to Cynodon dactylon and Phragmites communis: Cross-reactivity With Pooideae Grasses

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