Comparison of reactogenicity and antigenicity of M37 rotavirus vaccine and rhesus-rotavirus-based quadrivalent vaccine

Flores, Jorge; Kapikian, A Z; Pérez‐Schael, Irene; Blanco, Mireya González; White, Laura; Garcia, D; Vilar, Maria José Pereira; González, Rosabel; Urbina, Carlos Morado; Boher, J.; Méndez, Maritza Bericiartu; Cunto, Walter

doi:10.1016/0140-6736(90)91876-c

Cited by 60 publications

(18 citation statements)

References 29 publications

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“…We chose these two strains for several reasons. First, both strains have been used as heterologous backbone strains for human RV vaccines (10,17). Second, the RRV strain has a relatively unique phenotype vis-à-vis its ability to replicate efficiently in the murine biliary tree and cause a systemic liver and biliary tract disease in newborn or IFNR KO mice (8,21,23).…”

Section: Discussionmentioning

confidence: 99%

Roles of VP4 and NSP1 in Determining the Distinctive Replication Capacities of Simian Rotavirus RRV and Bovine Rotavirus UK in the Mouse Biliary Tract

Feng

Wolf

et al. 2011

J Virol

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Rotavirus replication and virulence are strongly influenced by virus strain and host species. The rotavirus proteins VP3, VP4, VP7, NSP1, and NSP4 have all been implicated in strain and species restriction of replication; however, the mechanisms have not been fully determined. Simian (RRV) and bovine (UK) rotaviruses have distinctive replication capacities in mouse extraintestinal organs such as the biliary tract. Using reassortants between UK and RRV, we previously demonstrated that the differential replication of these viruses in mouse embryonic fibroblasts is determined by the respective NSP1 proteins, which differ substantially in their abilities to degrade interferon (IFN) regulatory factor 3 (IRF3) and suppress the type I IFN response. In this study, we used an in vivo model of rotavirus infection of mouse gallbladder with UK ؋ RRV reassortants to study the genetic and mechanistic basis of systemic rotavirus replication. We found that the low-replication phenotype of UK in biliary tissues was conferred by UK VP4 and that the high-replication phenotype of RRV was conferred by RRV VP4 and NSP1. Viruses with RRV VP4 entered cultured mouse cholangiocytes more efficiently than did those with UK VP4. Reassortants with RRV VP4 and UK NSP1 genes induced high levels of expression of IRF3-dependent p54 in biliary tissues, and their replication was increased 3-fold in IFN-␣/␤ and -␥ receptor or STAT1 knockout (KO) mice compared to wild-type mice. Our data indicate that systemic rotavirus strain-specific replication in the murine biliary tract is determined by both viral entry mediated by VP4 and viral antagonism of the host innate immune response mediated by NSP1.Group A rotaviruses (RVs) are segmented double-stranded RNA viruses that replicate primarily in mature epithelial cells on the tips of small intestinal villi (7). Rotavirus is the most common cause of severe dehydrating diarrhea in infants and young children worldwide; these infections result in more than 600,000 deaths annually, mostly in developing countries, and over 2 million hospitalizations each year (20). Rotavirus infection is ubiquitous among mammals; however, virulent viral strains isolated from one animal species generally have a diminished replication capacity and diminished virulence in heterologous hosts. This phenomenon of rotavirus host range restriction was employed for the development of two rotavirus vaccines in which animal rotaviruses that are naturally attenuated in humans were used to produce the genetic backbone of human vaccines. The pentavalent rotavirus vaccine Rotateq (Merck), for example, is derived from a bovine rotavirus strain, WC, with incorporated human rotavirus genes that encode VP7 of serotypes 1, 2, 3, and 4 and VP4 of serotype P1A to induce neutralizing antibody (Ab) responses to the most common human RV serotypes.The viral factors underlying rotavirus host range restriction are not fully understood but are likely to be multigenic. Early studies using reassortants between simian and bovine strains demonstrated that the ...

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Section: Discussionmentioning

confidence: 99%

Roles of VP4 and NSP1 in Determining the Distinctive Replication Capacities of Simian Rotavirus RRV and Bovine Rotavirus UK in the Mouse Biliary Tract

Feng

Wolf

et al. 2011

J Virol

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“…However, both virulent Wa and live EDIM rotaviruses induced in the intestinal lamina propria IgA antibodies which were correlated with protection of mice and gnotobiotic pigs, respectively (15,28,71). The association between local (fecal) IgA antibody responses to HRV and protection against rotavirus disease has also been reported after natural rotavirus infection of children (20,42,43,55,68) and from some oral rotavirus vaccine trials in humans (4,30).…”

Section: Vol 74 2000mentioning

confidence: 98%

Intranasal Administration of 2/6-Rotavirus-Like Particles with MutantEscherichia coliHeat-Labile Toxin (LT-R192G) Induces Antibody-Secreting Cell Responses but Not Protective Immunity in Gnotobiotic Pigs

Yuan

Geyer

Hodgins

et al. 2000

J Virol

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We investigated the immunogenicity of recombinant double-layered rotavirus-like particle (2/6-VLPs) vaccines derived from simian SA11 or human (VP6) Wa and bovine RF (VP2) rotavirus strains. The 2/6-VLPs were administered to gnotobiotic pigs intranasally (i.n.) with a mutant Escherichia coli heat-labile toxin, LT-R192G (mLT) Rotaviruses are the leading cause of gastroenteritis in infants and young children worldwide (37). Rotavirus particles consist of triple-layered capsids containing a segmented double-stranded RNA genome. The rotavirus core is composed of VP2, which is the most abundant protein of the central core (15% of total virion mass) and a component of the RNA polymerase complex (26). The rotavirus major inner capsid protein is VP6, which is the most abundant structural protein of rotavirus (Ͼ50% of total virion mass) (26). VP6 is highly antigenic and contains antigenic determinants shared by all group A rotaviruses and antigenic determinants unique to the subgroup specificity. In general, most animal group A rotaviruses (including SA11) are subgroup I, whereas most human group A rotaviruses (including Wa) are subgroup II (34). The surface layer of the rotavirus capsid is composed of VP7 with VP4 spikes emanating from the outer surface (26). VP7 and VP4 independently induce virus-neutralizing (VN) antibodies (34). Diversity in VP4 and VP7 neutralizing antigenic determinants determines rotavirus P and G serotype specificity, respectively.,The viral proteins and determinants associated with protective immunity against rotavirus have not been fully delineated. Fecal or intestinal immunoglobulin A (IgA) antibodies or antibody-secreting cells (ASC) directed to rotavirus were suggested as correlates of protection in several studies of different species, including humans (15,20,28,42,64,71). Neutralizing antibodies to VP4 and VP7 were protective against rotavirus infection in mice, using monoclonal antibodies or recombinant

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“…This result indicated that the titer of vaccine viruses shed in stools declines after horizontal passage in infants [14,[29][30][31][32][33][34]. This may be of epidemiological importance because, in Caracas, Venezuela, unlike regions in temperate climate zone where rotavirus disappears in the warm/hot seasons, natural rotavirus transmission and disease occur year round [35].…”

Section: -10mentioning

confidence: 99%

Horizontal Transmission of Rhesus Monkey Rotavirus–Based Quadrivalent Vaccine during a Phase 3 Clinical Trial in Caracas, Venezuela

et al. 2003

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Comparison of reactogenicity and antigenicity of M37 rotavirus vaccine and rhesus-rotavirus-based quadrivalent vaccine

Cited by 60 publications

References 29 publications

Roles of VP4 and NSP1 in Determining the Distinctive Replication Capacities of Simian Rotavirus RRV and Bovine Rotavirus UK in the Mouse Biliary Tract

Roles of VP4 and NSP1 in Determining the Distinctive Replication Capacities of Simian Rotavirus RRV and Bovine Rotavirus UK in the Mouse Biliary Tract

Intranasal Administration of 2/6-Rotavirus-Like Particles with MutantEscherichia coliHeat-Labile Toxin (LT-R192G) Induces Antibody-Secreting Cell Responses but Not Protective Immunity in Gnotobiotic Pigs

Horizontal Transmission of Rhesus Monkey Rotavirus–Based Quadrivalent Vaccine during a Phase 3 Clinical Trial in Caracas, Venezuela

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